LOCUS BBa_K1179083 6483 bp DNA linear 14-Nov-2024 DEFINITION CAG_Cas9-VN155 ACCESSION BBa_K1179083 VERSION BBa_K1179083.1 FEATURES Location/Qualifiers promoter 1..1739 /label=BBa_K1179069 misc_feature 1749..1769 /label=attB1 misc_feature 1748..1769 /label=BBa_J85600 CDS 1778..6483 /label=BBa_K1179029 ORIGIN 1 gctccgaatt tctcgagtta attaatagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact 421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 481 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga 541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg 601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 661 gcgggcggga gtcgagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg 721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc 781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg 841 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg 901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc 961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg 1021 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg 1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc 1141 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg 1201 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg 1261 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag 1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga 1381 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc 1441 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct 1561 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg 1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca 1681 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcgacct 1741 actagagcca agtttgtaca aaaaagcagt actagaggga tcccggtacc ctcgacacct 1801 gctgacaggt cccttgccac catggacaag aagtactcca ttgggctcgc tatcggcaca 1861 aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa 1921 gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc 1981 gactccgggg agacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc 2041 cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg 2101 gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac 2161 gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca 2221 accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga cttgcggttg 2281 atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat cgagggggac 2341 ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat 2401 cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc aatcctgagc 2461 gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag 2521 aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc caactttaaa 2581 tctaacttcg acctggccga agatgccaag cttcaactga gcaaagacac ctacgatgat 2641 gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt tttggcggca 2701 aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac ggagatcacc 2761 aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca agacttgact 2821 ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat tttcttcgat 2881 cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga ggaattttac 2941 aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct ggtaaagctt 3001 aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat cccccaccag 3061 attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta cccctttttg 3121 aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta ctatgtaggc 3181 cccctcgccc ggggaaattc cagattcgcg tggatgactc gcaaatcaga agagacaatc 3241 actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc cttcatcgaa 3301 aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa acactctctg 3361 ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt cacagaaggg 3421 atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga cctcctcttc 3481 aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag actatttcaa aaagattgaa 3541 tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc cctgggaacg 3601 tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga ggagaacgag 3661 gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga gatgattgaa 3721 gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca gctcaagagg 3781 cgccgatata caggatgggg gcggctgtca agaaaactga tcaatgggat ccgagacaag 3841 cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa ccggaacttc 3901 atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa agcacaagtt 3961 tctggccagg gggacagtct tcacgagcac atcgctaatc ttgcaggtag cccagctatc 4021 aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg 4081 cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac ccagaaggga 4141 cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga actggggtcc 4201 caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa gctctacctg 4261 tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat caatcggctc 4321 tccgactacg acgtggatgc catcgtgccc cagtcttttc tcaaagatga ttctattgat 4381 aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt cccctcagaa 4441 gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa actgatcaca 4501 caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga gttggataaa 4561 gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca cgtggcccaa 4621 attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat tcgagaggtg 4681 aaagttatta ctctgaagtc taagctggtg tcagatttca gaaaggactt tcagttttat 4741 aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa tgcagtggta 4801 ggcactgcac ttatcaaaaa atatcccaag cttgaatctg aatttgttta cggagactat 4861 aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg caaggccacc 4921 gctaagtact tcttttacag caatattatg aattttttca agaccgagat tacactggcc 4981 aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg agaaatcgtg 5041 tgggacaagg gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc gcaggtgaac 5101 atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat cctcccgaaa 5161 aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa atacggcgga 5221 ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag 5281 tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga gcgatcaagc 5341 ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat ataaagaggt caaaaaagac 5401 ctcatcatta agcttcccaa gtactctctc tttgagcttg aaaacggccg gaaacgaatg 5461 ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc taaatacgtt 5521 aatttcttgt atctggccag ccactatgaa aagctcaaag ggtcccccga agataatgag 5581 cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat cgagcaaata 5641 agcgaattct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt gctttctgct 5701 tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat ccacttgttt 5761 actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac catagacaga 5821 aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca gtcaattacg 5881 gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag ggctgacccc 5941 aagaagaaga ggaaggtgtg tggtggttct ggtggtggtt ctggtatggt gagcaagggc 6001 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 6061 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 6121 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 6181 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc 6241 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 6301 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 6361 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 6421 tacaacagcc acaacgcgta tatcaccgcc tagtgagctt cagtgcaggt gaattctacc 6481 acc //