BBa_K1180002 1 BBa_K1180002 fusion protein of lamp 2b and RVG for brain targeting of the exosome 2013-09-23T11:00:00Z 2015-05-08T01:09:36Z The sequence is from the protein lamp 2b and RVG peptide. This part is designed to direct exosome to the brain. Lamp 2b (lysosomal associated membrane protein 2b) is a protein ubiquitously expressed on the surface of the exosomes. By genetically engineer a target protein to the outmembrane part of the lamp 2b, we can use the lamp 2b to bring our target protein to the surface of the exosome. Thus we can endow the exosome with site-specificity by the addition of the fusion protein.For brain targeting, we choose to use RVG, which is a short peptide from Rabies Virus, as our target protein. RVG can specifically recognize acetylcholine receptor in the central nervous system, thus we engineer the RVG peptide into the lamp 2b and we use pcDNA 3.1(+) as our vector. false false _1493_ 0 16730 9 Not in stock true We need to make the RVG peptide as short as possible to avoid the immune response which might be induced by the fusion protein. false Gong Fei ，Wang Wei， Wei Xiuqing BBa_K1180002_sequence 1 atgtgcctctctccggttaaaggcgcaaagctcatcctgatctttctgttcctaggagccgttcagtccaatgcattgatagttaatttgacagattcaaagggtacttgcctttatgctcgatacaccatttggatgcccgagaatccgagaccagggacaccttgtgacatttttaccaatagcagagggaagagagcatccaacgggtccggaggtgcagaatgggagatgaatttcacaataacatatgaaactacaaaccaaaccaataaaactataaccattgcagtacctgacaaggcgacacacgatggaagcagttgtggggatgaccggaatagtgccaaaataatgatacaatttggattcgctgtctcttgggctgtgaattttaccaaggaagcatctcattattcaattcatgacatcgtgctttcctacaacactagtgatagcacagtatttcctggtgctgtagctaaaggagttcatactgttaaaaatcctgagaatttcaaagttccattggatgtcatctttaagtgcaatagtgttttaacttacaacctgactcctgtcgttcagaaatattggggtattcacctgcaagcttttgtccaaaatggtacagtgagtaaaaatgaacaagtgtgtgaagaagaccaaactcccaccactgtggcacccatcattcacaccactgccccgtcgactacaactacactcactccaacttcaacacccactccaactccaactccaactccaaccgttggaaactacagcattagaaatggcaatactacctgtctgctggctaccatggggctgcagctgaacatcactgaggagaaggtgcctttcatttttaacatcaaccctgccacaaccaacttcaccggcagctgtcaacctcaaagtgctcaacttaggctgaacaacagccaaattaagtatcttgaccttatctttgctgtgaaaaatgaaaaacggttctatctgaaggaagtgaatgtctacatgtatttggctaatggctcagctttcaacatttccaacaagaaccttagcttctgggatgcccctctgggaagttcttatatgtgcaacaaagagcaggtgctttctgtgtctagagcgtttcagatcaacacctttaacctaaaggtgcaaccttttaatgtgacaaaaggacagtattctacagctcaagactgcagtgcagatgaagacaacttccttgtgcccatagcggtgggagcagctctgggaggagtacttattctagtgttgctggcttattttattggtctcaagcgccatcatactggatatgagcaattttagtaactgcaatctgattgatcatattacagaatacatacaaaaaaacaaagttgtcttacctgtcagtgtatgaagcactttgctccttaagacagacaccttggaactttcattcggaaattagtccccgaagtttgagatgagtctttttttgaatttttttttctctgatctgcttaaaatgcagattggctttaaaaagaaaagaaaacaaacacacatatgcaatgttttaaggtctgtcttaagaagctttggccaaattgggatcctaacctaagatgccttaaacttattaacatgaccattataagaaaagatatatggagttgtatcttactggaattaataaacactgcttgaccacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z