LOCUS       BBa_K118020             1923 bp    DNA     linear       22-Dec-2024
DEFINITION  Plac+rbs+glgC16 (glgC with G336D substitution))
ACCESSION   BBa_K118020
VERSION     BBa_K118020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     misc_feature    1630..1632
                     /label=G336D
     misc_feature    1195..1200
                     /label=EcoRI site removed
     misc_feature    1692..1697
                     /label=EcoRI site removed
     CDS             625..1923
                     /label=BBa_K118016
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatggtt agtttagaga agaacgatca cttaatgttg
      661 gcgcgccagc tgccattgaa atctgttgcc ctgatactgg cgggaggacg tggtacccgc
      721 ctgaaggatt taaccaataa gcgagcaaaa ccggccgtac acttcggcgg taagttccgc
      781 attatcgact ttgcgctgtc taactgcatc aactccggga tccgtcgtat gggcgtgatc
      841 acccagtacc agtcccacac tctggtgcag cacattcagc gcggctggtc attcttcaat
      901 gaagaaatga acgagtttgt cgatctgctg ccagcacagc agagaatgaa aggggaaaac
      961 tggtatcgcg gcaccgcaga tgcggtcacc caaaacctcg acattatccg ccgttataaa
     1021 gcggaatacg tggtgatcct ggcgggcgac catatctaca agcaagacta ctcgcgtatg
     1081 cttatcgatc acgtcgaaaa aggcgcacgt tgcaccgttg cttgtatgcc agtaccgatt
     1141 gaagaagcct ccgcatttgg cgttatggcg gttgatgaga acgataaaat tatcgaattt
     1201 gttgaaaaac ctgctaaccc gccgtcaatg ccgaacgatc cgagcaaatc tctggcgagt
     1261 atgggtatct acgtctttga cgccgactat ctgtatgaac tgctggaaga agacgatcgc
     1321 gatgagaact ccagccacga ctttggcaaa gatttgattc ccaagatcac cgaagccggt
     1381 ctggcctatg cgcacccgtt cccgctctct tgcgtacaat ccgacccgga tgccgagccg
     1441 tactggcgcg atgtgggtac gctggaagct tactggaaag cgaacctcga tctggcctct
     1501 gtggtgccgg aactggatat gtacgatcgc aattggccaa ttcgcaccta caatgaatca
     1561 ttaccgccag cgaaattcgt gcaggatcgc tccggtagcc acgggatgac ccttaactca
     1621 ctggtttccg acggttgtgt gatctccggt tcggtggtgg tgcagtccgt tctgttctcg
     1681 cgcgttcgcg tgaactcatt ctgcaacatt gattccgccg tattgttacc ggaagtatgg
     1741 gtaggtcgct cgtgccgtct gcgccgctgc gtcatcgatc gtgcttgtgt tattccggaa
     1801 ggcatggtga ttggtgaaaa cgcagaggaa gatgcacgtc gtttctatcg ttcagaagaa
     1861 ggcatcgtgc tggtaacgcg cgaaatgcta cggaagttag ggcataaaca ggagcgataa
     1921 taa
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