##gff-version 3
##sequence-region BBa_K118020 1 1923
BBa_K118020	.	ribosome_entry_site	609	618	.	+	0	ID=BBa_J15001;Name=BBa_J15001
BBa_K118020	.	sequence_feature	609	611	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K118020	.	ribosome_entry_site	612	618	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K118020	.	engineered_region	1	600	.	+	0	ID=BBa_J33207;Name=BBa_J33207
BBa_K118020	.	operator	248	285	.	+	0	ID=annotation1907855;Name=CAP binding site;Parent=BBa_J33207
BBa_K118020	.	promoter	320	325	.	+	0	ID=annotation1907859;Name=-10;Parent=BBa_J33207
BBa_K118020	.	CDS	370	600	.	+	0	ID=annotation1907858;Name=lacZ';Parent=BBa_J33207
BBa_K118020	.	sequence_feature	1	3	.	+	0	ID=annotation1907854;Name=SacI;Parent=BBa_J33207
BBa_K118020	.	ribosome_entry_site	359	362	.	+	0	ID=annotation1907857;Name=rbs;Parent=BBa_J33207
BBa_K118020	.	promoter	297	302	.	+	0	ID=annotation1907860;Name=-35;Parent=BBa_J33207
BBa_K118020	.	operator	332	352	.	+	0	ID=annotation1907856;Name=LacI binding site;Parent=BBa_J33207
BBa_K118020	.	CDS	625	1923	.	+	0	ID=BBa_K118016;Name=BBa_K118016
BBa_K118020	.	point_mutation	1692	1697	.	+	0	ID=annotation1978559;Name=EcoRI site removed;Parent=BBa_K118016
BBa_K118020	.	point_mutation	1195	1200	.	+	0	ID=annotation1978558;Name=EcoRI site removed;Parent=BBa_K118016
BBa_K118020	.	sequence_alteration	1630	1632	.	+	0	ID=annotation1978557;Name=G336D;Parent=BBa_K118016
>BBa_K118020
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggc
cgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcc
agctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttg
cctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagctcaaggaggtactagatggttag
tttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgt
ggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgact
ttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgca
gcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatg
aaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaat
acgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgc
acgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgat
aaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgg
gtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactt
tggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatcc
gacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctg
tggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgt
gcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtg
gtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtat
gggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtga
aaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaag
ttagggcataaacaggagcgataataa