LOCUS BBa_K118024 4354 bp DNA linear 22-Nov-2024 DEFINITION dxs+LIMS1+appY ACCESSION BBa_K118024 VERSION BBa_K118024.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI RBS 4..10 /label=rbs RBS 1..10 /label=BBa_J15001 CDS 17..1879 /label=dxs coding sequence CDS 17..1882 /label=BBa_K118000 misc_feature 1891..1893 /label=SacI RBS 1894..1900 /label=rbs RBS 1891..1900 /label=BBa_J15001 CDS 1907..3577 /label=(+) limonene synthase coding region misc_feature 1907..1909 /label=start misc_feature 3572..3577 /label=stop CDS 1907..3577 /label=BBa_I742110 misc_feature 3586..3588 /label=SacI RBS 3589..3595 /label=rbs RBS 3586..3595 /label=BBa_J15001 CDS 3602..4351 /label=appY coding sequence CDS 3602..4354 /label=BBa_K118001 ORIGIN 1 ctcaaggagg tactagatga gttttgatat tgccaaatac ccgaccctgg cactggtcga 61 ctccacccag gagttacgac tgttgccgaa agagagttta ccgaaactct gcgacgaact 121 gcgccgctat ttactcgaca gcgtgagccg ttccagcggg cacttcgcct ccgggctggg 181 cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac accccgtttg accaattgat 241 ttgggatgtg gggcatcagg cttatccgca taaaattttg accggacgcc gcgacaaaat 301 cggcaccatc cgtcagaaag gcggtctgca cccgttcccg tggcgcggcg aaagcgaata 361 tgacgtatta agcgtcgggc attcatcaac ctccatcagt gccggaattg gtattgcggt 421 tgctgccgaa aaagaaggca aaaatcgccg caccgtctgt gtcattggcg atggcgcgat 481 taccgcaggc atggcgtttg aagcgatgaa tcacgcgggc gatatccgtc ctgatatgct 541 ggtgattctc aacgacaatg aaatgtcgat ttccgaaaat gtcggcgcgc tcaacaacca 601 tctggcacag ctgctttccg gtaagcttta ctcttcactg cgcgaaggcg ggaaaaaagt 661 tttctctggc gtgccgccaa ttaaagagct gctcaaacgc accgaagaac atattaaagg 721 catggtagtg cctggcacgt tgtttgaaga gctgggcttt aactacatcg gcccggtgga 781 cggtcacgat gtgctggggc ttatcaccac gctaaagaac atgcgcgacc tgaaaggccc 841 gcagttcctg catatcatga ccaaaaaagg tcgtggttat gaaccggcag aaaaagaccc 901 gatcactttc cacgccgtgc ctaaatttga tccctccagc ggttgtttgc cgaaaagtag 961 cggcggtttg ccgagctatt caaaaatctt tggcgactgg ttgtgcgaaa cggcagcgaa 1021 agacaacaag ctgatggcga ttactccggc gatgcgtgaa ggttccggca tggtcgagtt 1081 ttcacgtaaa ttcccggatc gctacttcga cgtggcaatt gccgagcaac acgcggtgac 1141 ctttgctgcg ggtctggcga ttggtgggta caaacccatt gtcgcgattt actccacttt 1201 cctgcaacgc gcctatgatc aggtgctgca tgacgtggcg attcaaaagc ttccggtcct 1261 gttcgccatc gaccgcgcgg gcattgttgg tgctgacggt caaacccatc agggtgcttt 1321 tgatctctct tacctgcgct gcataccgga aatggtcatt atgaccccga gcgatgaaaa 1381 cgaatgtcgc cagatgctct ataccggcta tcactataac gatggcccgt cagcggtgcg 1441 ctacccgcgt ggcaacgcgg tcggcgtgga actgacgccg ctggaaaaac taccaattgg 1501 caaaggcatt gtgaagcgtc gtggcgagaa actggcgatc cttaactttg gtacgctgat 1561 gccagaagcg gcgaaagtcg ccgaatcgct gaacgccacg ctggtcgata tgcgttttgt 1621 gaaaccgctt gatgaagcgt taattctgga aatggccgcc agccatgaag cgctggtcac 1681 cgtagaagaa aacgccatta tgggcggcgc aggcagcggc gtgaacgaag tgctgatggc 1741 ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg gacttcttta ttccgcaagg 1801 aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc gctggtatgg aagccaaaat 1861 caaggcctgg ctggcataat aatactagag ctcaaggagg tactagatgc gtcgttcagc 1921 aaactaccaa ccttcaattt gggaccacga ttttttgcag tcattgaata gcaactatac 1981 ggatgaagca tacaaaagac gagcagaaga gctgagggga aaagtgaaga tagcgattaa 2041 ggatgtaatc gagcctctgg atcagttgga gctgattgat aacttgcaaa gacttggatt 2101 ggctcatcgt tttgagactg agattaggaa catattgaat aatatctaca acaataataa 2161 agattataat tggagaaaag aaaatctgta tgcaacctcc cttgagttta gactacttag 2221 acaacatggc tatcctgttt ctcaagaggt tttcaatggt tttaaagacg accagggagg 2281 cttcatttgt gatgatttca agggaatact gagcttgcat gaagcttcgt attacagctt 2341 agaaggagaa agcatcatgg aggaggcctg gcaatttacc agcaaacatc ttaaagaagt 2401 gatgatcagc aagaacatgg aagaggatgt atttgtagca gaacaagcga agcgtgcact 2461 ggaactgcct ctgcattgga aagtgccaat gttagaggca aggtggttca tacacattta 2521 tgagagaaga gaggacaaga accacctttt acttgaactg gctaagatgg agtttaacac 2581 tttgcaggca atttaccagg aagaactaaa agaaatttca gggtggtgga aggatacagg 2641 tcttggagag aaattgagct ttgcgaggaa caggttggta gcgtccttct tatggagcat 2701 ggggatcgcg tttgagcctc aattcgccta ttgtcggaga gtgctcacaa tctcgatagc 2761 cctaattaca gtgattgatg acatttatga tgtctatgga acattggatg aacttgagat 2821 attcactgat gctgttgaga ggtgggacat caattatgct ttgaagcacc ttccgggcta 2881 tatgaaaatg tgttttcttg cgctttacaa ctttgttaat gaatttgctt attacgttct 2941 caaacaacag gattttgatt tgcttctgag cataaaaaat gcatggcttg gcttaataca 3001 agcctacttg gtggaggcga aatggtacca tagcaagtac acaccgaaac tggaagaata 3061 cttggaaaat ggattggtat caataacggg ccctttaatt ataacgattt catatctttc 3121 tggtacaaat ccaatcatta agaaggaact ggaatttctg gaaagtaatc cagatatagt 3181 tcactggtca tccaagattt tccgtctgca agatgatttg ggaacttcat cggacgagat 3241 acagagaggg gatgttccga aatcaatcca gtgttacatg catgaaactg gtgcctcaga 3301 ggaagttgct cgtcaacaca tcaaggatat gatgagacag atgtggaaga aggtgaatgc 3361 atacacagcc gataaagact ctcccttgac tggaacaact actgagttcc tcttgaatct 3421 tgtgagaatg tcccatttta tgtatctaca tggagatggg catggtgttc aaaaccaaga 3481 gactatcgat gtcggtttta cattgctttt tcagcccatt cccttggagg acaaacacat 3541 ggctttcaca gcatctcctg gcaccaaagg ctaataatac tagagctcaa ggaggtacta 3601 gatggattat gtttgctccg tagttttcat ctgtcaatca tttgatttaa ttataaacag 3661 gagagttatc tcgttcaaaa aaaattcatt gtttattgta agcgacaaaa ttagaaggga 3721 gttaccagta tgcccctcta aactaagaat tgttgatata gataagaaaa catgtttatc 3781 cttttttatc gacgtgaata atgagctgcc tggcaaattt actcttgata agaatggcta 3841 tattgctgaa gaggaacctc cattatcgct tgttttttct ctgtttgaag ggattaaaat 3901 agcagactca cactcccttt ggttaaaaga aagactatgt atatccttac ttgccatgtt 3961 caaaaaacgc gaaagtgtaa attcatttat actaacaaat ataaatacat ttacctgtaa 4021 aattactgga ataatcagtt ttaatattga gcggcaatgg catttaaaag atattgcgga 4081 attgatttat acgagtgaaa gtttaataaa aaaaagatta agggatgaag gaacgtcatt 4141 tactgaaata ttgagagata ctaggatgag gtatgcaaaa aaactcataa cttcaaactc 4201 ttattctatc aatgtcgtag cccagaaatg tggctataac agtacttcat atttcatatg 4261 tgcatttaaa gattattatg gtgtcacgcc atctcattat tttgagaaaa taatcggcgt 4321 cacagatgga ataaacaaaa caattgacta ataa //