BBa_K118025
type: ComponentDefinition
persistent identity: BBa K118025
display id: BBa_K118025
version: 1
was generated by: iGEM to SBOL conversion
description: limonene generator with lac promoter
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
mutable provenance: The lac promoter, dxs and appY are from E. coli; LIMS1 is from lemon.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part consists of a lac promoter (with lacZ' tag for blue-white selection) coupled to the limonene generation system consisting of dxs (deoxyxylulose phosphate synthase, rate controlling step of terpenoid biosynthesis in bacteria) + LIMS1 (limonene synthase 1 from lemon) + appY (a transcription factor known to increase terpenoid biosynthesis).
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Chris French
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K118001
component: BBa_J15001
component: BBa_K118000
component: BBa_I742110
component: BBa_J33207
sequence annotation: BBa_J33207
sequence annotation: BBa_J15001
sequence annotation: BBa_K118000
sequence annotation: BBa_I742110
sequence annotation: BBa_K118001
sequence: https://synbiohub.org/public/igem/BBa_K118025_sequence/1

BBa_K118001
type: Component
persistent identity: component1985174
display id: component1985174
version: 1
top level: BBa_K118025
definition: appY
access: public

BBa_J15001
type: Component
persistent identity: component1985166
display id: component1985166
version: 1
top level: BBa_K118025
definition: BBa_J15001
access: public

BBa_K118000
type: Component
persistent identity: component1985163
display id: component1985163
version: 1
top level: BBa_K118025
definition: dxs
access: public

BBa_J15001
type: Component
persistent identity: component1985173
display id: component1985173
version: 1
top level: BBa_K118025
definition: BBa_J15001
access: public

BBa_I742110
type: Component
persistent identity: component1985170
display id: component1985170
version: 1
top level: BBa_K118025
definition: LIMS1
access: public

BBa_J15001
type: Component
persistent identity: component1985162
display id: component1985162
version: 1
top level: BBa_K118025
definition: BBa_J15001
access: public

BBa_J33207
type: Component
persistent identity: component1985159
display id: component1985159
version: 1
top level: BBa_K118025
definition: BBa_J33207
access: public

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1985159
display id: annotation1985159
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985159/range1985159/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K118025/annotation1985159/range1985159/1
type: Range
persistent identity: range1985159
display id: range1985159
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 1
end: 600
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985162
display id: annotation1985162
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985162/range1985162/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985162/range1985162/1
type: Range
persistent identity: range1985162
display id: range1985162
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 609
end: 618
orientation: inline

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation1985163
display id: annotation1985163
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985163/range1985163/1
component: BBa_K118000

https://synbiohub.org/public/igem/BBa_K118025/annotation1985163/range1985163/1
type: Range
persistent identity: range1985163
display id: range1985163
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 625
end: 2490
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985166
display id: annotation1985166
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985166/range1985166/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985166/range1985166/1
type: Range
persistent identity: range1985166
display id: range1985166
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 2499
end: 2508
orientation: inline

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1985170
display id: annotation1985170
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985170/range1985170/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_K118025/annotation1985170/range1985170/1
type: Range
persistent identity: range1985170
display id: range1985170
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 2515
end: 4185
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985173
display id: annotation1985173
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985173/range1985173/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985173/range1985173/1
type: Range
persistent identity: range1985173
display id: range1985173
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 4194
end: 4203
orientation: inline

BBa_K118001
type: SequenceAnnotation
persistent identity: annotation1985174
display id: annotation1985174
version: 1
top level: BBa_K118025
location: https://synbiohub.org/public/igem/BBa_K118025/annotation1985174/range1985174/1
component: BBa_K118001

https://synbiohub.org/public/igem/BBa_K118025/annotation1985174/range1985174/1
type: Range
persistent identity: range1985174
display id: range1985174
version: 1
top level: BBa_K118025
direction: direction/reverse
start: 4210
end: 4962
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_J33207
type: ComponentDefinition
persistent identity: BBa J33207
display id: BBa_J33207
version: 1
was generated by: iGEM to SBOL conversion
description: lac promoter and lacZ
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct.
owned by: james
owned by: myers
mutable description: This part (submitted in pSB1A2) consists of the lac promoter and lacZ' gene, encoding the N-terminal 76 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation for blue-white selection on Xgal plates. A SacI site has been introduced at the 3' end, overlapping the XbaI site of the Biobrick prefix. This is designed to be used as a cloning vector for making new biobricks. PCR primers can be designed with a SacI site in one primer and an SpeI site in the other. This removes the necessity for an excessively long non-complementary tail on one primer bearing either the full biobrick prefix or suffix. The PCR product can then be digested with SacI and SpeI for insertion into this plasmid, replacing the Plac-lacZ' cassette. Recombinant plasmids will then be white on IPTG/Xgal plates, whereas any that still contain the original insert will be blue. We have used this strategy to prepare several biobricks, including BBa_J33204, which contains the xylE gene encoding catechol-2,3-dioxygenase. (For making biobricks that contain lacZ', BBa_J33204 can be used in the same way; in this case, clones with plasmids that still contain xylE will turn yellow on addition of a drop of 10 mM catechol.)
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: SacI
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: rbs
sequence annotation: lacZ'
sequence: https://synbiohub.org/public/igem/BBa_J33207_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1907854
display id: annotation1907854
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J33207/annotation1907854/range1907854/1
type: Range
persistent identity: range1907854
display id: range1907854
version: 1
top level: BBa_J33207
direction: direction/forward
start: 1
end: 3
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1907855
display id: annotation1907855
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J33207/annotation1907855/range1907855/1
type: Range
persistent identity: range1907855
display id: range1907855
version: 1
top level: BBa_J33207
direction: direction/forward
start: 248
end: 285
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1907860
display id: annotation1907860
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33207/annotation1907860/range1907860/1
type: Range
persistent identity: range1907860
display id: range1907860
version: 1
top level: BBa_J33207
direction: direction/forward
start: 297
end: 302
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1907859
display id: annotation1907859
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J33207/annotation1907859/range1907859/1
type: Range
persistent identity: range1907859
display id: range1907859
version: 1
top level: BBa_J33207
direction: direction/forward
start: 320
end: 325
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1907856
display id: annotation1907856
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J33207/annotation1907856/range1907856/1
type: Range
persistent identity: range1907856
display id: range1907856
version: 1
top level: BBa_J33207
direction: direction/forward
start: 332
end: 352
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1907857
display id: annotation1907857
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J33207/annotation1907857/range1907857/1
type: Range
persistent identity: range1907857
display id: range1907857
version: 1
top level: BBa_J33207
direction: direction/forward
start: 359
end: 362
orientation: inline

lacZ'
type: SequenceAnnotation
persistent identity: annotation1907858
display id: annotation1907858
version: 1
top level: BBa_J33207
location: https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J33207/annotation1907858/range1907858/1
type: Range
persistent identity: range1907858
display id: range1907858
version: 1
top level: BBa_J33207
direction: direction/forward
start: 370
end: 600
orientation: inline

dxs
type: ComponentDefinition
persistent identity: BBa K118000
display id: BBa_K118000
version: 1
was generated by: iGEM to SBOL conversion
description: dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of dxs from Escherichia coli JM109. It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses teh first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Chris French
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dxs coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118000_sequence/1

dxs coding sequence
type: SequenceAnnotation
persistent identity: annotation1995699
display id: annotation1995699
version: 1
top level: dxs
location: https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
type: Range
persistent identity: range1995699
display id: range1995699
version: 1
top level: dxs
direction: direction/forward
start: 1
end: 1863
orientation: inline

appY
type: ComponentDefinition
persistent identity: BBa K118001
display id: BBa_K118001
version: 1
was generated by: iGEM to SBOL conversion
description: appY coding sequence encoding a DNA-binding transcriptional activator
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of appY from Escherichia coli JM109. It encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005).
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: appY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118001_sequence/1

appY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995700
display id: annotation1995700
version: 1
top level: appY
location: https://synbiohub.org/public/igem/BBa_K118001/annotation1995700/range1995700/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118001/annotation1995700/range1995700/1
type: Range
persistent identity: range1995700
display id: range1995700
version: 1
top level: appY
direction: direction/forward
start: 1
end: 750
orientation: inline

LIMS1
type: ComponentDefinition
persistent identity: BBa I742110
display id: BBa_I742110
version: 1
was generated by: iGEM to SBOL conversion
description: LIMS1
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
mutable description: Limonene synthase 1 from Citrus limon (accession AF514287) Synthesised by Geneart. Limonene synthase converts farnesyl-diphosphate to + limonene, which is a component of lemon scent.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: (+) limonene synthase coding region
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I742110_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1949773
display id: annotation1949773
version: 1
top level: LIMS1
location: https://synbiohub.org/public/igem/BBa_I742110/annotation1949773/range1949773/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I742110/annotation1949773/range1949773/1
type: Range
persistent identity: range1949773
display id: range1949773
version: 1
top level: LIMS1
direction: direction/forward
start: 1
end: 3
orientation: inline

(+) limonene synthase coding region
type: SequenceAnnotation
persistent identity: annotation1949772
display id: annotation1949772
version: 1
top level: LIMS1
location: https://synbiohub.org/public/igem/BBa_I742110/annotation1949772/range1949772/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742110/annotation1949772/range1949772/1
type: Range
persistent identity: range1949772
display id: range1949772
version: 1
top level: LIMS1
direction: direction/forward
start: 1
end: 1671
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1949774
display id: annotation1949774
version: 1
top level: LIMS1
location: https://synbiohub.org/public/igem/BBa_I742110/annotation1949774/range1949774/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I742110/annotation1949774/range1949774/1
type: Range
persistent identity: range1949774
display id: range1949774
version: 1
top level: LIMS1
direction: direction/forward
start: 1666
end: 1671
orientation: inline

https://synbiohub.org/public/igem/BBa_K118025_sequence/1
type: Sequence
persistent identity: BBa K118025 sequence
display id: BBa_K118025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagctcaaggaggtactagatgagttttgatattgccaaatacccgaccctggcactggtcgactccacccaggagttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccgttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgtttgaccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcaccatccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatcaacctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcattggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtgattctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttccggtaagctttactcttcactgcgcgaaggcgggaaaaaagttttctctggcgtgccgccaattaaagagctgctcaaacgcaccgaagaacatattaaaggcatggtagtgcctggcacgttgtttgaagagctgggctttaactacatcggcccggtggacggtcacgatgtgctggggcttatcaccacgctaaagaacatgcgcgacctgaaaggcccgcagttcctgcatatcatgaccaaaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactttccacgccgtgcctaaatttgatccctccagcggttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaatctttggcgactggttgtgcgaaacggcagcgaaagacaacaagctgatggcgattactccggcgatgcgtgaaggttccggcatggtcgagttttcacgtaaattcccggatcgctacttcgacgtggcaattgccgagcaacacgcggtgacctttgctgcgggtctggcgattggtgggtacaaacccattgtcgcgatttactccactttcctgcaacgcgcctatgatcaggtgctgcatgacgtggcgattcaaaagcttccggtcctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaaacccatcagggtgcttttgatctctcttacctgcgctgcataccggaaatggtcattatgaccccgagcgatgaaaacgaatgtcgcca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33207_sequence/1
type: Sequence
persistent identity: BBa J33207 sequence
display id: BBa_J33207_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118000_sequence/1
type: Sequence
persistent identity: BBa K118000 sequence
display id: BBa_K118000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagttttgatattgccaaatacccgaccctggcactggtcgactccacccaggagttacgactgttgccgaaagagagtttaccgaaactctgcgacgaactgcgccgctatttactcgacagcgtgagccgttccagcgggcacttcgcctccgggctgggcacggtcgaactgaccgtggcgctgcactatgtctacaacaccccgtttgaccaattgatttgggatgtggggcatcaggcttatccgcataaaattttgaccggacgccgcgacaaaatcggcaccatccgtcagaaaggcggtctgcacccgttcccgtggcgcggcgaaagcgaatatgacgtattaagcgtcgggcattcatcaacctccatcagtgccggaattggtattgcggttgctgccgaaaaagaaggcaaaaatcgccgcaccgtctgtgtcattggcgatggcgcgattaccgcaggcatggcgtttgaagcgatgaatcacgcgggcgatatccgtcctgatatgctggtgattctcaacgacaatgaaatgtcgatttccgaaaatgtcggcgcgctcaacaaccatctggcacagctgctttccggtaagctttactcttcactgcgcgaaggcgggaaaaaagttttctctggcgtgccgccaattaaagagctgctcaaacgcaccgaagaacatattaaaggcatggtagtgcctggcacgttgtttgaagagctgggctttaactacatcggcccggtggacggtcacgatgtgctggggcttatcaccacgctaaagaacatgcgcgacctgaaaggcccgcagttcctgcatatcatgaccaaaaaaggtcgtggttatgaaccggcagaaaaagacccgatcactttccacgccgtgcctaaatttgatccctccagcggttgtttgccgaaaagtagcggcggtttgccgagctattcaaaaatctttggcgactggttgtgcgaaacggcagcgaaagacaacaagctgatggcgattactccggcgatgcgtgaaggttccggcatggtcgagttttcacgtaaattcccggatcgctacttcgacgtggcaattgccgagcaacacgcggtgacctttgctgcgggtctggcgattggtgggtacaaacccattgtcgcgatttactccactttcctgcaacgcgcctatgatcaggtgctgcatgacgtggcgattcaaaagcttccggtcctgttcgccatcgaccgcgcgggcattgttggtgctgacggtcaaacccatcagggtgcttttgatctctcttacctgcgctgcataccggaaatggtcattatgaccccgagcgatgaaaacgaatgtcgccagatgctctataccggctatcactataacgatggcccgtcagcggtgcgctacccgcgtggcaacgcggtcggcgtggaactgacgccgctggaaaaactaccaattggcaaaggcattgtgaagcgtcgtggcgagaaactggcgatccttaactttggtacgctgatgccagaagcggcgaaagtcgccgaatcgctgaacgccacgctggtcgatatgcgttttgtgaaaccgcttgatgaagcgttaattctggaaatggccgccagccatgaagcgctggtcaccgtagaagaaaacgccattatgggcggcgcaggcagcggcgtgaacgaagtgctgatggcccatcgtaaaccagtacccgtgctgaacattggcctgccggacttctttattccgcaaggaactcaggaagaaatgcgcgccgaactcggcctcgatgccgctggtatggaagccaaaatcaaggcctggctggcataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118001_sequence/1
type: Sequence
persistent identity: BBa K118001 sequence
display id: BBa_K118001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattatgtttgctccgtagttttcatctgtcaatcatttgatttaattataaacaggagagttatctcgttcaaaaaaaattcattgtttattgtaagcgacaaaattagaagggagttaccagtatgcccctctaaactaagaattgttgatatagataagaaaacatgtttatccttttttatcgacgtgaataatgagctgcctggcaaatttactcttgataagaatggctatattgctgaagaggaacctccattatcgcttgttttttctctgtttgaagggattaaaatagcagactcacactccctttggttaaaagaaagactatgtatatccttacttgccatgttcaaaaaacgcgaaagtgtaaattcatttatactaacaaatataaatacatttacctgtaaaattactggaataatcagttttaatattgagcggcaatggcatttaaaagatattgcggaattgatttatacgagtgaaagtttaataaaaaaaagattaagggatgaaggaacgtcatttactgaaatattgagagatactaggatgaggtatgcaaaaaaactcataacttcaaactcttattctatcaatgtcgtagcccagaaatgtggctataacagtacttcatatttcatatgtgcatttaaagattattatggtgtcacgccatctcattattttgagaaaataatcggcgtcacagatggaataaacaaaacaattgactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742110_sequence/1
type: Sequence
persistent identity: BBa I742110 sequence
display id: BBa_I742110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtcgttcagcaaactaccaaccttcaatttgggaccacgattttttgcagtcattgaatagcaactatacggatgaagcatacaaaagacgagcagaagagctgaggggaaaagtgaagatagcgattaaggatgtaatcgagcctctggatcagttggagctgattgataacttgcaaagacttggattggctcatcgttttgagactgagattaggaacatattgaataatatctacaacaataataaagattataattggagaaaagaaaatctgtatgcaacctcccttgagtttagactacttagacaacatggctatcctgtttctcaagaggttttcaatggttttaaagacgaccagggaggcttcatttgtgatgatttcaagggaatactgagcttgcatgaagcttcgtattacagcttagaaggagaaagcatcatggaggaggcctggcaatttaccagcaaacatcttaaagaagtgatgatcagcaagaacatggaagaggatgtatttgtagcagaacaagcgaagcgtgcactggaactgcctctgcattggaaagtgccaatgttagaggcaaggtggttcatacacatttatgagagaagagaggacaagaaccaccttttacttgaactggctaagatggagtttaacactttgcaggcaatttaccaggaagaactaaaagaaatttcagggtggtggaaggatacaggtcttggagagaaattgagctttgcgaggaacaggttggtagcgtccttcttatggagcatggggatcgcgtttgagcctcaattcgcctattgtcggagagtgctcacaatctcgatagccctaattacagtgattgatgacatttatgatgtctatggaacattggatgaacttgagatattcactgatgctgttgagaggtgggacatcaattatgctttgaagcaccttccgggctatatgaaaatgtgttttcttgcgctttacaactttgttaatgaatttgcttattacgttctcaaacaacaggattttgatttgcttctgagcataaaaaatgcatggcttggcttaatacaagcctacttggtggaggcgaaatggtaccatagcaagtacacaccgaaactggaagaatacttggaaaatggattggtatcaataacgggccctttaattataacgatttcatatctttctggtacaaatccaatcattaagaaggaactggaatttctggaaagtaatccagatatagttcactggtcatccaagattttccgtctgcaagatgatttgggaacttcatcggacgagatacagagaggggatgttccgaaatcaatccagtgttacatgcatgaaactggtgcctcagaggaagttgctcgtcaacacatcaaggatatgatgagacagatgtggaagaaggtgaatgcatacacagccgataaagactctcccttgactggaacaactactgagttcctcttgaatcttgtgagaatgtcccattttatgtatctacatggagatgggcatggtgttcaaaaccaagagactatcgatgtcggttttacattgctttttcagcccattcccttggaggacaaacacatggctttcacagcatctcctggcaccaaaggctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z