LOCUS BBa_K1185004 5210 bp DNA linear 25-Nov-2024 DEFINITION Integration vector for Bacillus subtilis derived from pSac-Cm ACCESSION BBa_K1185004 VERSION BBa_K1185004.1 FEATURES Location/Qualifiers misc_feature 23..151 /label=B0015 CDS 167..970 /label='sacA CDS 1306..2176 /label=bla CDS 3167..3922 /label='sacA CDS 4231..4894 /label=cat ORIGIN 1 gaattcgcgg ccgcttctag agccaggcat caaataaaac gaaaggctca gtcgaaagac 61 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 121 tcaccttcgg gtgggccttt ctgcgtttat attaagcttc taggatcctg agcgccggtc 181 gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc 241 ccgtatttcg cgtaaggaaa tccattatgt actatttcga tcagaccagt ttttaatttg 301 tgtgtttcca tgtgtccagt ttggcgcgcc ccatccgacc attgactgcc actggattca 361 ctgcatgacg ctgccgagac agctgacgct ttcaggacag aagctgattc agcagccgct 421 gcctgagcta aaagccatgc gcagaaatga gaaaaaaata cacatcaaca tgcatggatc 481 atctggtgcg cttccagtgg aaaaacctga aagaactgag attctactgg aagacattca 541 tacggagtct ggcttttcaa tcagtatccg cggaacggct acgttttcct tccataaaga 601 cgaggggatt gttacgctgg aacgaaagag ctttgacgga aaaagaacag aagcgagaca 661 ttgccgcatc aaggatttgc ataccgtaca catgtttctc gacgcgtcat ctgtggaaat 721 ctttatcaat aacggagaag aggtctttag tgcaagatat tttcctttcc cgggaaatca 781 tgaagtaaca gccagtgcga ccgggaaatc ctggcgtaat agcgaagagg cccgcaccga 841 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct 901 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 961 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 1021 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 1081 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 1141 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 1201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 1261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 1321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 1381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 1441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 1501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 1561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 1621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 1681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 2041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 2101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 2161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 2221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 2281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 2341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 2401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 2461 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 2521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 2641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 2701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 3001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 3061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 3121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggacagc 3181 acatgaccag gagcttcgtc gccgggctta tgaagaagtg gagaaaaaag agcccatcgc 3241 taacagcgat ccgcaccgcc agcattttca tatcatgccg ccggttgggc tgctgaatga 3301 cccgaatggc gtgatttatt ggaagggcag ctatcatgta ttctttcagt ggcagccgtt 3361 tcagacgggg cacggcgcaa aattttgggg gcattatacg acacaggatg ttgtgaattg 3421 gaagcgggaa gagattgcgc tggctccgag tgattggttt gataaaaacg gctgctactc 3481 gggcagcgct gtcacgaaag acgatcggct ctatcttttt tacacaggaa atgtcaggga 3541 tcaggatgga aatcgggaaa cgtatcaatg ccttgctgtt tctgacgacg ggctgtcctt 3601 tgagaaaaag ggtgtcgtcg cccgccttcc ggaagcgata ttaacggcgc acttttcgcg 3661 atccgaagta tgggagcatg aaggcacatg gtatatggtg attggtgcgc aaacagagaa 3721 tttgaaaggg caggctgtgt tgtttgcttc tgataacctg acagagtgga gatttcttgg 3781 cccgataacc ggcgcgggct tcaacgggct ggacgatttt ggcgcgccta aacggatatc 3841 atcatcgctc atccatgtcg acgctctccc ttatgcgact cctgcattag gaagcagccc 3901 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg 3961 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc 4021 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca 4081 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg 4141 agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt agaaaaccga 4201 ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta ggcctatctg 4261 acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa acagaatgat 4321 gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat tttcctgctg 4381 taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac ccagtaaatg 4441 aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt ttgggaaaca 4501 atttccccga accattatat ttctctacat cagaaaggta taaatcataa aactctttga 4561 agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca tcaaaaattg 4621 tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg taaccagttc 4681 taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca gggtaaaatt 4741 tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct gtggttatac 4801 taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc caattgtcta 4861 aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa agtgaattta 4921 ggaggcttac ttgtctgctt tcttcattag aatcaatcct tttttaaaag tcaatattac 4981 tgtaacataa atatatattt taaaaatatc ccactttatc caattttcgt ttgttgaact 5041 aatgggtgct ttagttgaag aataaagacc acattaaaaa atgtggtctt ttgtgttttt 5101 ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt atcttgataa taagggtaac 5161 tattgccgat gataagctgt caaacatgat actagtagcg gccgctgcag //