LOCUS       BBa_K1189000            2692 bp    DNA     linear       16-Feb-2025
DEFINITION  TALE-A with a his tag under a lacI promoter
ACCESSION   BBa_K1189000
VERSION     BBa_K1189000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    229..240
                     /label=BBa_K1189022
     CDS             227..2692
                     /label=TALE A
     misc_feature    230..247
                     /label=His-tag
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc accaccacca
      241 ccaccacgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
      301 tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggcattcacc gcggggtacc
      361 tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa tcaagcctaa
      421 ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct tcactcatgc
      481 gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg tcaaatacca
      541 agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg tcggtaaaca
      601 gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc ttagggggcc
      661 tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg gagtaacagc
      721 ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga acctgacccc
      781 agaccaggta gtcgcaatcg cgtcaaacgg agggggaaag caagccctgg aaaccgtgca
      841 aaggttgttg ccggtccttt gtcaagacca cggccttaca ccggagcaag tcgtggccat
      901 tgcaagcaat gggggtggca aacaggctct tgagacggtt cagagacttc tcccagttct
      961 ctgtcaagcc cacgggctga ctcccgatca agttgtagcg attgcgtcga acattggagg
     1021 gaaacaagca ttggagactg tccaacggct ccttcccgtg ttgtgtcaag cccacggttt
     1081 gacgcctgca caagtggtcg ccatcgccag ccatgatggc ggtaagcagg cgctggaaac
     1141 agtacagcgc ctgctgcctg tactgtgcca ggatcatgga ctgaccccag accaggtagt
     1201 cgcaatcgcg tcaaacggag ggggaaagca agccctggaa accgtgcaaa ggttgttgcc
     1261 ggtcctttgt caagaccacg gccttacacc ggagcaagtc gtggccattg caaataataa
     1321 cggtggcaaa caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca
     1381 cgggctgact cccgatcaag ttgtagcgat tgcgtcgcat gacggaggga aacaagcatt
     1441 ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca
     1501 agtggtcgcc atcgcctcga atggcggcgg taagcaggcg ctggaaacag tacagcgcct
     1561 gctgcctgta ctgtgccagg atcatggact gaccccagac caggtagtcg caatcgcgaa
     1621 caataatggg ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca
     1681 agaccacggc cttacaccgg agcaagtcgt ggccattgca tcccacgacg gtggcaaaca
     1741 ggctcttgag acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc
     1801 cgatcaagtt gtagcgattg cgtccaacgg tggagggaaa caagcattgg agactgtcca
     1861 acggctcctt cccgtgttgt gtcaagccca cggtttgacg cctgcacaag tggtcgccat
     1921 cgccagccat gatggcggta agcaggcgct ggaaacagta cagcgcctgc tgcctgtact
     1981 gtgccaggat catggactga ccccagacca ggtagtcgca atcgcgtcac atgacggggg
     2041 aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc ctttgtcaag accacggcct
     2101 tacaccggag caagtcgtgg ccattgcatc ccacgacggt ggcaaacagg ctcttgagac
     2161 ggttcagaga cttctcccag ttctctgtca agcccacggg ctgactcccg atcaagttgt
     2221 agcgattgcg aataacaatg gagggaaaca agcattggag actgtccaac ggctccttcc
     2281 cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg gtcgccatcg ccagccatga
     2341 tggcggtaag caggcgctgg aaacagtaca gcgcctgctg cctgtactgt gccaggatca
     2401 tggactgaca cccgaacagg tggtcgccat tgcttctaat gggggaggac ggccagcctt
     2461 ggagtccatc gtagcccaat tgtccaggcc cgatcccgcg ttggctgcgt taacgaatga
     2521 ccatctggtg gcgttggcat gtcttggtgg acgacccgcg ctcgatgcag tcaaaaaggg
     2581 tctgcctcat gctcccgcat tgatcaaaag aaccaaccgg cggattcccg agagaacttc
     2641 ccatcgagtc gcgggatccg atccaaaaaa gaagagaaag gtataatcta gg
//