LOCUS       BBa_K1189007            1112 bp    DNA     linear       25-Nov-2024
DEFINITION  Beta-lactamase with His Tag under the control of the inducible 
            lacI promoter
ACCESSION   BBa_K1189007
VERSION     BBa_K1189007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    229..231
                     /label=Start
     misc_feature    229..1086
                     /label=BBa_K1189008
     misc_feature    1095..1112
                     /label=PolyHis
     misc_feature    1095..1112
                     /label=BBa_J18909
     misc_feature    940..945
                     /label=BsaI cut site (not removed)
     CDS             229..1112
                     /label=BBa_K1189009
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagag tattcaacat
      241 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      301 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttatatc
      361 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      421 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      481 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      541 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      601 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      661 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      721 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      781 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      841 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      901 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg tatcattgca
      961 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1021 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1081 tggtaatact agagcatcat catcatcatc at
//