LOCUS       BBa_K1189015            1039 bp    DNA     linear       25-Nov-2024
DEFINITION  E coil with a GFP under a lacI promoters
ACCESSION   BBa_K1189015
VERSION     BBa_K1189015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     CDS             229..288
                     /label=E-Coil
     CDS             295..1014
                     /label=GFP protein
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ccggcgaaat
      241 cgcggccctg gagaaagaaa ttgctgcgct ggaaaaggaa atcgctgcac tggagaaaac
      301 cggccgtaag ggtgaagaac tgttcacggg tgttgtacca atcctggttg aactggatgg
      361 tgacgtaaac ggtcataagt tcagcgtgtc tggtgaaggt gaaggcgatg caacttacgg
      421 taaactgacg ctgaagttca tctgcactac cggcaaactg cctgtaccat ggccgactct
      481 ggttactact tttggttatg gtgttcagtg tttcgcacgt tacccggatc atatgaagca
      541 gcatgacttc ttcaagagcg ctatgccgga aggttatgtg caagagcgta ccatcttctt
      601 caaagacgac ggtaactaca aaacccgcgc tgaagtcaaa ttcgagggcg ataccctggt
      661 taaccgtatc gaactgaaag gcatcgactt taaagaggac ggcaacatcc tgggtcacaa
      721 actggagtac aactacaact cccacaacgt gtacatcatg gccgacaaac agaaaaacgg
      781 catcaaagtg aactttaaaa ttcgccacaa cattgaagac ggctccgtgc agctggcgga
      841 ccactaccag cagaacaccc cgattggcga tggcccggtt ctgctgccgg ataatcacta
      901 tctgtctacc caatctgctc tgtccaaaga tccgaatgaa aaacgtgatc acatggttct
      961 gctggaattt gtcaccgcgg cgggcattac ccacggcatg gatgaactgt ataaagatta
     1021 caaagatgac gacgacaaa
//