LOCUS BBa_K1189021 3793 bp DNA linear 23-Dec-2024 DEFINITION TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker ACCESSION BBa_K1189021 VERSION BBa_K1189021.1 FEATURES Location/Qualifiers misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI misc_feature 209..220 /label=BBa_B0034 CDS 227..247 /label=hisTag CDS 227..2671 /label=TALE A CDS 2672..2716 /label=GS Linker CDS 2717..3265 /label=Heavy-Chain Ferritin Subunit CDS 3266..3793 /label=Light-Chain Ferritin Subunit ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc accaccacca 241 ccaccacgac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga 301 tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggcattcacc gcggggtacc 361 tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa tcaagcctaa 421 ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct tcactcatgc 481 gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg tcaaatacca 541 agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg tcggtaaaca 601 gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc ttagggggcc 661 tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg gagtaacagc 721 ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga acctgacccc 781 agaccaggta gtcgcaatcg cgtcaaacgg agggggaaag caagccctgg aaaccgtgca 841 aaggttgttg ccggtccttt gtcaagacca cggccttaca ccggagcaag tcgtggccat 901 tgcaagcaat gggggtggca aacaggctct tgagacggtt cagagacttc tcccagttct 961 ctgtcaagcc cacgggctga ctcccgatca agttgtagcg attgcgtcga acattggagg 1021 gaaacaagca ttggagactg tccaacggct ccttcccgtg ttgtgtcaag cccacggttt 1081 gacgcctgca caagtggtcg ccatcgccag ccatgatggc ggtaagcagg cgctggaaac 1141 agtacagcgc ctgctgcctg tactgtgcca ggatcatgga ctgaccccag accaggtagt 1201 cgcaatcgcg tcaaacggag ggggaaagca agccctggaa accgtgcaaa ggttgttgcc 1261 ggtcctttgt caagaccacg gccttacacc ggagcaagtc gtggccattg caaataataa 1321 cggtggcaaa caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca 1381 cgggctgact cccgatcaag ttgtagcgat tgcgtcgcat gacggaggga aacaagcatt 1441 ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca 1501 agtggtcgcc atcgcctcga atggcggcgg taagcaggcg ctggaaacag tacagcgcct 1561 gctgcctgta ctgtgccagg atcatggact gaccccagac caggtagtcg caatcgcgaa 1621 caataatggg ggaaagcaag ccctggaaac cgtgcaaagg ttgttgccgg tcctttgtca 1681 agaccacggc cttacaccgg agcaagtcgt ggccattgca tcccacgacg gtggcaaaca 1741 ggctcttgag acggttcaga gacttctccc agttctctgt caagcccacg ggctgactcc 1801 cgatcaagtt gtagcgattg cgtccaacgg tggagggaaa caagcattgg agactgtcca 1861 acggctcctt cccgtgttgt gtcaagccca cggtttgacg cctgcacaag tggtcgccat 1921 cgccagccat gatggcggta agcaggcgct ggaaacagta cagcgcctgc tgcctgtact 1981 gtgccaggat catggactga ccccagacca ggtagtcgca atcgcgtcac atgacggggg 2041 aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc ctttgtcaag accacggcct 2101 tacaccggag caagtcgtgg ccattgcatc ccacgacggt ggcaaacagg ctcttgagac 2161 ggttcagaga cttctcccag ttctctgtca agcccacggg ctgactcccg atcaagttgt 2221 agcgattgcg aataacaatg gagggaaaca agcattggag actgtccaac ggctccttcc 2281 cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg gtcgccatcg ccagccatga 2341 tggcggtaag caggcgctgg aaacagtaca gcgcctgctg cctgtactgt gccaggatca 2401 tggactgaca cccgaacagg tggtcgccat tgcttctaat gggggaggac ggccagcctt 2461 ggagtccatc gtagcccaat tgtccaggcc cgatcccgcg ttggctgcgt taacgaatga 2521 ccatctggtg gcgttggcat gtcttggtgg acgacccgcg ctcgatgcag tcaaaaaggg 2581 tctgcctcat gctcccgcat tgatcaaaag aaccaaccgg cggattcccg agagaacttc 2641 ccatcgagtc gcgggatccg atccaaaaaa gggtggaggt ggatcaggtg gaggtggatc 2701 aggaggtgga ggctcaatga ctaccgccag cactagccag gttcgccaga actaccatca 2761 ggactccgag gctgcaatca accgtcagat caacctggaa ctgtacgcgt cttatgtcta 2821 cctgtctatg agctattact tcgaccgtga tgacgttgca ctgaaaaact tcgcgaaata 2881 cttcctgcat cagtctcatg aagaacgcga gcacgctgaa aaactgatga aactgcaaaa 2941 ccagcgtggt ggccgtattt ttctgcaaga cattaaaaaa ccggattgcg acgattggga 3001 gtctggtctg aacgccatgg aatgcgcact gcacctggag aaaaacgtga atcaatccct 3061 gctggagctg cacaagctgg ccaccgacaa aaacgatccg cacctgtgtg atttcatcga 3121 aacccactac ctgaatgagc aggtgaaagc tatcaaagaa ctgggtgacc acgtaaccaa 3181 cctgcgtaag atgggtgcgc cggaatccgg cctggcggaa tatctgtttg acaaacacac 3241 gctgggcgat tccgataacg aatctatgag ctcccagatc cgtcagaact acagcaccga 3301 cgtagaagca gctgtgaact ccctggtcaa tctgtacctg caagccagct atacgtacct 3361 gtctctgggt ttctatttcg atcgtgacga tgtggcgctg gaaggcgttt ctcacttctt 3421 ccgcgaactg gctgaagaaa aacgtgaggg ttacgaacgt ctgctgaaaa tgcagaacca 3481 acgtggcggt cgtgccctgt tccaggacat taagaaaccg gcggaagatg agtggggtaa 3541 aactccggat gctatgaaag cagcgatggc actggagaag aaactgaacc aggcgctgct 3601 ggacctgcat gctctgggtt ctgctcgcac tgacccgcac ctgtgcgatt ttctggagac 3661 ccacttcctg gacgaagaag ttaaactgat caagaaaatg ggcgaccacc tgaccaacct 3721 gcaccgtctg ggcggtccag aagcgggcct gggtgaatac ctgtttgagc gcctgaccct 3781 gaaacatgat taa //