LOCUS       BBa_K1189031            3574 bp    DNA     linear       22-Nov-2024
DEFINITION  TALE-A linked to beta-lactamase with a his tag under a lacI 
            promoter
ACCESSION   BBa_K1189031
VERSION     BBa_K1189031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    89..126
                     /label=CAP
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     misc_feature    221..226
                     /label=RFC10_codingScar
     CDS             227..247
                     /label=hexaHisTag
     CDS             248..2662
                     /label=TALEA
     CDS             2669..2713
                     /label=GGGGS(*3)_linker
     CDS             2663..2668
                     /label=KasI_cutSite
     CDS             2714..3574
                     /label=beta_lactamase
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcaccacca
      241 ccatcatgat tataaagacc atgatggaga ctataaagat catgatatcg actacaaaga
      301 tgatgacgat aagatggcac cgaaaaaaaa acgcaaagtg ggtatccacc gcggtgtgcc
      361 tatggtggac ctgcggaccc ttggatattc gcaacaacag caagagaaaa ttaaaccgaa
      421 ggttcgctcg acggtggcgc aacatcatga ggccttagtg gggcatggct ttactcatgc
      481 ccatattgtg gctttgtccc agcatccggc ggccctcggt actgttgcag taaaatacca
      541 agatatgatt gcagcgctgc ctgaagcaac ccacgaagcg attgtggggg ttggaaagca
      601 gtggtctggt gctcgcgccc tggaggcatt gctgaccgtg gccggggaac tgcgcggacc
      661 accgcttcag ttggacaccg gtcagttgct gaaaattgca aaacggggcg gagtaacggc
      721 agtggaggcc gtgcatgcgt ggcgcaacgc cctgaccggc gcacctctga atctgacccc
      781 ggatcaggta gtggcaatcg cctctaatgg aggcggcaaa caggcgctgg aaaccgtgca
      841 gcgtctgtta cccgtgctgt gtcaggatca tggcctgacc ccagaacaag tggtcgcaat
      901 cgcgtcaaat ggaggcggaa aacaggccct cgaaaccgtg caacgcctct tgccggtact
      961 gtgccaggca cacggtctga cccccgatca ggtggttgcg atcgcaagca acattggtgg
     1021 caagcaggcg ctggaaaccg tgcaacgtct tttacccgtc ctgtgccagg cccatggatt
     1081 aacccctgca caggtggtgg cgattgcaag ccatgacggc ggcaaacagg cgctggaaac
     1141 cgtgcagcgc ctgctgcccg tattatgcca ggatcacggc ctgactcccg atcaagtggt
     1201 cgcaatcgca tccaacggcg ggggcaaaca ggcgctggaa acggtccaac gtctgttacc
     1261 ggtcctgtgt caggatcatg gtctgactcc ggagcaggtt gtggcaattg ctaataataa
     1321 tgggggcaaa caagcgctcg aaacagtgca gcggctgctg cccgtgctgt gccaggcgca
     1381 tggactgacc cctgatcagg ttgtggccat tgcgagccac gacggaggga aacaagcatt
     1441 agaaaccgtt caacgcttac tgccagtgct gtgtcaggct catggcctga cccccgcgca
     1501 ggttgttgcg attgcgtcca acggcggcgg caaacaagct ctggagacag ttcagcgtct
     1561 gcttcccgtt ctgtgccaag atcatggtct gacccccgac caagtcgttg ccatcgccaa
     1621 caataatggc ggcaagcagg cactggaaac agtacagcgt cttttgccgg tgctttgcca
     1681 ggaccacggg ttaaccccag agcaggtggt tgcgatcgcg tctcatgacg gcgggaaaca
     1741 agcactggaa actgtccagc gtttgctgcc ggttttgtgc caagcccatg ggctgacgcc
     1801 ggaccaagtc gtggcgatcg caagtaacgg cgggggaaaa caagctctgg aaacagtaca
     1861 gcgcttactt cccgtgttat gccaggcaca tgggctgact ccagcccaag tggtggcgat
     1921 cgctagccat gatgggggca aacaggcact ggagactgta cagcgtttac tcccggtttt
     1981 gtgccaggac catggcctga ctccggacca agtggtagcg atcgcgtccc acgacggcgg
     2041 taaacaggcg ctggagacgg tgcagcgttt acttccggtg ctctgtcaag atcatggact
     2101 gacacctgaa caagttgttg caattgcatc tcatgatgga ggtaagcagg cgttggaaac
     2161 ggtgcagcgc ctgctcccgg tgctgtgtca ggctcacggc ttgacaccag atcaggtcgt
     2221 cgcaattgcc aataataatg gaggcaagca ggcgttggaa actgtccagc gtctgttgcc
     2281 agtgctttgt caggcacacg gcttgactcc ggcgcaggtc gtcgctattg cgagccatga
     2341 cggtggtaag caggcattag agactgttca acgtctcctg ccggttcttt gtcaggatca
     2401 cggtctgact cctgaacagg ttgtcgcgat cgcatccaat ggtggtggcc gtcccgcgct
     2461 tgaaagtatt gttgctcagt tgagccgccc ggacccagca cttgccgcac ttacgaatga
     2521 ccaccttgta gcactggcgt gcttgggtgg acgcccggca ctggatgcgg tcaagaaagg
     2581 tttgccccac gctccggcac tcattaagcg cacgaatcgt cggattccgg aacggaccag
     2641 ccaccgggtg gccggctctg atggcgccgg aggtggtggc agtggaggtg gaggttctgg
     2701 aggtggtggc tctatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2761 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2821 tcagttgggt gcacgagtgg gttatatcga actggatctc aacagcggta agatccttga
     2881 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     2941 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3001 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3061 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3121 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3181 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3241 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3301 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3361 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3421 tgagcgtggg tcccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3481 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3541 tgagataggt gcctcactga ttaagcattg gtaa
//