LOCUS       BBa_K1189032            2662 bp    DNA     linear       15-Jun-2024
DEFINITION  EHEC TALE1 (binds to 19bp of stx2 gene)
ACCESSION   BBa_K1189032
VERSION     BBa_K1189032.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=R0010
     misc_feature    221..226
                     /label=RFC10_codingScar
     CDS             227..248
                     /label=hisTag
     RBS             209..220
                     /label=B0034
     CDS             249..2662
                     /label=EHEC_TALE1
     misc_feature    2546..2551
                     /label=KasI
     misc_feature    2597..2659
                     /label=Kcoil
     misc_feature    2552..2596
                     /label=GS_linker
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcaccatca
      241 tcaccatatg caacaccacg aagccttagt tggtcacggc ttcactcatg ctcacattgt
      301 cgctctttct cagcacccag cagcacttgg cacggtggcg gtcacatatc agcatattat
      361 cacggcactg ccagaagcga cgcatgaaga tatcgtgggt gtcggtaaac aatggagcgg
      421 ggcacgcgcg ctggaagcat tgctgaccgt cgctggcgag ctccggggac caccactgca
      481 actggatacg ggtcagctgc tgaagatcgc taaacgcggc ggcgtgacgg ctgtggaagc
      541 agtgcatgct tggcgcaatg cgctgacagg cgctccactg aacctgacac cggaacaggt
      601 tgttgccatt gcgtcaaacg gcggaggtaa acaggcactg gaaactgtcc aacgtttact
      661 gcccgttctg tgtcaggcgc atggtctgac gcctcagcaa gtggtggcga tcgcgtccaa
      721 cggcgggggc aaacaggcgt tagaaaccgt ccagcgtctg cttccggttc tttgccaagc
      781 tcatgggttg accccgcagc aggttgtagc tatcgcatcg catgatggtg gaaaacaggc
      841 actcgaaacc gtccaacggc tgcttccagt gctctgtcag gcacatggtt taactccgga
      901 gcaggttgtg gcgatcgcgt cgaacggcgg cggtaaacag gccttagaaa ccgttcaacg
      961 tctgctgccc gtactttgtc aggctcatgg tttgactccg gaacaggtag tcgccattgc
     1021 ctcaaacatt ggtggcaaac aggcgctgga aacggttcaa gcgttgctcc ctgtcttatg
     1081 tcaggctcac ggtttaacac cggagcaggt tgtggccatc gcatcgcatg atgggggaaa
     1141 acaggcgctg gagacagtcc aggccctgct gcctgtcctg tgccaggcac atgggctgac
     1201 cccggaacaa gtcgtggcaa tcgcaagcca tgatggggga aaacaggcat tagaaaccgt
     1261 gcaggcgttg ctgcccgttc tgtgccaggc gcatggcctc acgccggaac aggtggtggc
     1321 gattgcatcc aacaacgggg gcaaacaagc gttggaaacc gtccaacgtc tgttgccggt
     1381 tctttgtcag gcccacggcc ttactcctga gcaggttgtg gcaattgcgt caaacggcgg
     1441 tgggaaacag gcactggaaa cggtacaacg tctgctgcct gtactttgtc aagcccacgg
     1501 attgactccg caacaagttg ttgccatcgc ctctaacggc ggtggtaaac aggccctgga
     1561 aaccgtacag cgtctgttgc cggtgctgtg ccaagctcac ggtttaactc cggaacaagt
     1621 tgttgccatc gccagtaacg gcggtggaaa acaggccctg gaaacggttc aagcccttct
     1681 gcctgtgtta tgtcaagcgc acggtttaac tccggagcaa gttgttgcga ttgcctctaa
     1741 cggcgggggc aaacaggccc ttgaaaccgt gcagcgcctg ttgcctgtgt tatgtcaggc
     1801 acacggcctc acgcctgagc aggttgtggc tattgcgtcg aacggcgggg gaaagcaggc
     1861 attagaaacg gtgcagcgtc tgctgccggt gctttgccag gcacacggac ttacccccga
     1921 gcaggtagtg gccatcgcgt cccatgatgg cggcaaacag gctctggaaa cggttcagcg
     1981 ccttttacct gtgctgtgcc aggcacatgg tttgacacca gagcaggtcg ttgccattgc
     2041 ctccaacatt ggtggcaaac aagcactgga aactgtccag cgtcttctgc ctgtgttgtg
     2101 tcaggctcat ggtctgacac cacagcaggt tgtagcaatc gcctctaaca acgggggtcg
     2161 ccctgcgctg gaaacagtgc agcgtttgct gcctgtactc tgccaggccc atgggttgac
     2221 gccagagcag gtggtagcta ttgcctcaaa cattggcggg aaacaggcac tggaaacagt
     2281 gcaacggctc ctgcctgttt tgtgccaagc tcatggcctc acgccacaac aagtggtggc
     2341 aattgcctca aacggcggcg gccgtccggc cttagaatcc attgttgcgc aactgagccg
     2401 cccggatccg gcgctcgccg ctttgaccaa tgaccatctg gttgcccttg cgtgcctggg
     2461 cggtcgcccg gcgatggatg ctgttaaaaa gggattaccg cacgcaccag aactgatccg
     2521 ccgtgtgaat cgtcgcccgg atcctggcgc cggtggagga ggttctggag gcggtggaag
     2581 tggtggcgga ggtagcaaaa tcgcggcgct gaaagagaaa atcgcggcgc tgaaagagaa
     2641 aatcgcggcg ctgaaagaat aa
//