LOCUS       BBa_K1189033            2662 bp    DNA     linear       25-Dec-2024
DEFINITION  EHEC TALE2 (binds to a 19bp region of stx2 gene)
ACCESSION   BBa_K1189033
VERSION     BBa_K1189033.1  
FEATURES             Location/Qualifiers
     misc_feature    1..220
                     /label=BBa_J04500
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..247
                     /label=hisTag
     CDS             248..2551
                     /label=EHEC TALE2
     CDS             2552..2596
                     /label=GS_linker
     CDS             2597..2659
                     /label=K_coil
     misc_feature    2546..2551
                     /label=KasI cut site
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcaccatca
      241 ccatcacatg caacaccacg aagccttagt tggtcacggc ttcactcatg ctcacattgt
      301 cgctctttct cagcacccag cagcacttgg cacggtggcg gtcacatatc agcatattat
      361 cacggcactg ccagaagcga cgcatgaaga tatcgtgggt gtcggtaaac aatggagcgg
      421 ggcacgcgcg ctggaagcat tgctgaccgt cgctggcgag ctccggggac caccactgca
      481 actggatacg ggtcagctgc tgaagatcgc taaacgcggc ggcgtgacgg ctgtggaagc
      541 agtgcatgct tggcgcaatg cgctgacagg cgctccactg aacctgacac cggaacaggt
      601 tgttgccatt gcgtcacatg atggaggtaa acaggcactg gaaactgtcc aacgtttact
      661 gcccgttctg tgtcaggcac atggtctgac gcctcagcaa gttgttgcaa ttgcgtccaa
      721 cggcgggggc aaacaggcat tagaaaccgt ccagcgcctg cttccggttc tttgccaagc
      781 tcatgggttg accccgcagc aggttgtagc tatcgcatcg catgatggtg gaaaacaggc
      841 actcgaaacc gtccaacggc tgcttccagt gctctgtcag gcacatggtt taactccgga
      901 gcaggttgtg gcgatcgcgt cgaacggcgg cggtaaacag gccttagaaa ccgttcaacg
      961 tctgctgccc gtactttgtc aggctcatgg tttgactccg gaacaggtag tcgccattgc
     1021 ctcaaacaac ggtggcaaac aggcactgga aacggttcaa gcgttgctcc ctgtcttatg
     1081 tcaggctcac ggtttaacac cggagcaggt tgtggccatc gcatcgaacg gcgggggaaa
     1141 acaggcactg gagacagtcc aggccctgct gcctgtcctg tgccaggcac atgggctgac
     1201 cccggaacaa gtcgtggcaa tcgcaagcca tgatggggga aaacaggcat tagaaaccgt
     1261 gcaggcgttg ctgcccgttc tgtgccaagc gcacggtctg actccagaac aggtggtggc
     1321 gattgcatcc catgatgggg gcaaacaagc gttggaaacc gtccaacgcc tgttgccggt
     1381 tctttgtcag gcccacggcc ttactcctga gcaggttgtg gcaattgcgt caaacaacgg
     1441 tgggaaacag gcgctggaaa cggtacaacg tctgctgcct gtactttgtc aagcccacgg
     1501 attgactccg caacaagttg ttgccatcgc ctctaacggc ggtggtaaac aggccctgga
     1561 aaccgtacag cgtctgttgc cggtgctgtg ccaagctcac ggtttaacac cagagcaggt
     1621 tgttgccatc gccagtaaca ttggtggaaa acaggccctg gaaacggttc aagcccttct
     1681 gcctgtgtta tgtcaggcgc atgggttaac tccggagcaa gttgttgcga ttgcctctaa
     1741 cggcgggggc aaacaggccc ttgaaaccgt gcagcgtctg ttgcctgtgt tatgtcaggc
     1801 acacggcctc acgcctgagc aggttgtggc tattgcgtcg aacattgggg gaaaacaagc
     1861 gttagaaacg gtgcagcgtc tgctgccggt gctttgccag gcacacggac ttacccccga
     1921 gcaggtagtg gccatcgcgt cccacgatgg cggcaaacag gctctggaaa cggttcagcg
     1981 tcttttacct gttctgtgtc aagcgcatgg cttgacaccg gagcaggtcg ttgccattgc
     2041 ctccaacggc ggtggcaaac aagcactgga aactgtccag cgtcttctgc ctgtgttgtg
     2101 tcaggctcat ggtctgacac cacagcaggt tgtagcaatc gcctctaaca ttgggggtcg
     2161 ccctgcactg gaaacagtgc agcgcttgct gcctgtactc tgccaggccc atgggttgac
     2221 gccagagcag gtggtagcta ttgcctcaaa cggcggcggg aaacaggcac tggaaacagt
     2281 gcaacggctc ctgcctgttt tgtgccaagc tcatggcctc accccacagc aagtggtggc
     2341 aattgcctca aacggcggcg gccgtccggc cttagaatcc attgttgcgc aactgagccg
     2401 cccggatccg gcgctcgccg ctttgaccaa tgaccatctg gttgcccttg cgtgcctggg
     2461 cggtcgcccg gcgatggatg ctgttaaaaa gggattaccg cacgcaccag aactgatccg
     2521 ccgtgtgaat cgtcgcccgg atcctggcgc cggtggagga ggttctggag gcggtggaag
     2581 tggtggcgga ggtagcaaaa tcgcggcact gaaagagaaa atcgcggcac tgaaagagaa
     2641 aatcgcggct ctgaaagaat aa
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