BBa_K119010 1 BBa_K119010 LuxR_cI_aiiA_Normal lacZ promoter 2008-09-30T11:00:00Z 2015-05-08T01:09:38Z We based this part in BBa_I729006 from Chiba team This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA false false _235_ 0 3054 9 Not in stock false We needed a repressor which could be induced by a "signal" in order to inhibit the RcnA(BBa_K119003, BBa_K119009) activity. We achieved this by taking advantage of the LuxR promoter present in the Chiba device BBa_I729006. So, we placed cI downstream of this promoter and changed the promoter which was controlling aiiA for the LacZ one. false Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja,Martin del castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, E annotation1977973 1 BBa_C0062 range1977973 1 83 863 annotation1977997 1 aiiA range1977997 1 1907 2656 annotation1977991 1 LVA range1977991 1 1800 1838 annotation1977968 1 -35 range1977968 1 21 27 annotation1977970 1 -10 range1977970 1 45 50 annotation1978003 1 polya range1978003 1 2844 2857 annotation1977989 1 BBa_C0051 range1977989 1 1089 1838 annotation1977986 1 RBS-3\Weak range1977986 1 1072 1084 annotation1977976 1 BBa_B0010 range1977976 1 872 951 annotation1978002 1 BBa_B0012 range1978002 1 2823 2843 annotation1977988 1 RBS range1977988 1 1078 1081 annotation1977995 1 BBa_C0060 range1977995 1 1907 2720 annotation1977977 1 stem_loop range1977977 1 883 926 annotation1977981 1 stop range1977981 1 993 995 annotation1977975 1 luxR range1977975 1 83 833 annotation1978000 1 BBa_B0010 range1978000 1 2729 2808 annotation1977980 1 polya range1977980 1 987 1000 annotation1977979 1 T7 TE range1977979 1 967 986 annotation1977972 1 conserved range1977972 1 67 70 annotation1977971 1 BBa_B0034 range1977971 1 65 76 annotation1977967 1 BBa_R0040 range1977967 1 3 22 annotation1978004 1 stop range1978004 1 2850 2852 annotation1977969 1 TetR 2 range1977969 1 28 56 annotation1977987 1 BBa_B0032 range1977987 1 1072 1084 annotation1977994 1 BBa_B0034 range1977994 1 1889 1900 annotation1977990 1 cI lambda range1977990 1 1089 1799 annotation1977998 1 LVA range1977998 1 2686 2718 annotation1977999 1 2 range1977999 1 2690 2692 annotation1977984 1 -35 range1977984 1 1028 1033 annotation1977974 1 prefix range1977974 1 83 84 annotation1977983 1 LuxR/HSL range1977983 1 1009 1029 annotation1978001 1 stem_loop range1978001 1 2740 2783 annotation1977982 1 BBa_R0062 range1977982 1 1009 1063 annotation1977985 1 -10 range1977985 1 1050 1055 annotation1977992 1 LacZ promoter range1977992 1 1845 1882 annotation1977978 1 BBa_B0012 range1977978 1 960 1000 annotation1977966 1 TetR 1 range1977966 1 1 19 annotation1977996 1 start range1977996 1 1907 1909 annotation1977993 1 BBa_K119000 range1977993 1 1845 1882 BBa_K119010_sequence 1 ggtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagcgcgatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataatgcgcatttacactttatgcttccggctcgtatgttgtgtggacctagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccaatgcattggtttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z