LOCUS       BBa_K119011             2873 bp    DNA     linear       29-Nov-2024
DEFINITION  LuxR_cI_aiiA_Mutated lacZ promoter
ACCESSION   BBa_K119011
VERSION     BBa_K119011.1  
FEATURES             Location/Qualifiers
     misc_feature    3..22
                     /label=BBa_R0040
     misc_feature    3..22
                     /label=TetR 1
     promoter        21..27
                     /label=-35
     misc_feature    28..48
                     /label=TetR 2
     promoter        45..50
                     /label=-10
     misc_feature    65..76
                     /label=BBa_B0034
     misc_feature    67..70
                     /label=conserved
     CDS             83..833
                     /label=luxR
     misc_feature    83..84
                     /label=prefix
     misc_feature    83..863
                     /label=BBa_C0062
     misc_feature    872..951
                     /label=BBa_B0010
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    960..1000
                     /label=BBa_B0012
     stem_loop       967..986
                     /label=T7 TE
     polyA_site      987..1000
                     /label=polya
     misc_feature    993..995
                     /label=stop
     misc_feature    1009..1063
                     /label=BBa_R0062
     misc_feature    1009..1029
                     /label=LuxR/HSL
     promoter        1028..1033
                     /label=-35
     promoter        1050..1055
                     /label=-10
     RBS             1072..1084
                     /label=RBS-3\Weak
     misc_feature    1072..1084
                     /label=BBa_B0032
     RBS             1078..1081
                     /label=RBS
     misc_feature    1089..1838
                     /label=BBa_C0051
     CDS             1089..1799
                     /label=cI lambda
     misc_feature    1800..1838
                     /label=LVA
     promoter        1845..1882
                     /label=LacZ promoter
     misc_feature    1845..1882
                     /label=BBa_K119001
     misc_feature    1889..1900
                     /label=BBa_B0034
     misc_feature    1907..2720
                     /label=BBa_C0060
     misc_feature    1907..1909
                     /label=start
     CDS             1907..2656
                     /label=aiiA
     misc_feature    2686..2718
                     /label=LVA
     misc_feature    2690..2692
                     /label=2
     misc_feature    2729..2808
                     /label=BBa_B0010
     stem_loop       2740..2783
                     /label=stem_loop
     misc_feature    2823..2843
                     /label=BBa_B0012
     polyA_site      2844..2857
                     /label=polya
     misc_feature    2850..2852
                     /label=stop
     misc_feature    1873..1873
                     /label=mutation
ORIGIN
        1 ggtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact
       61 agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat acagaataat
      121 taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac
      181 taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa
      241 atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc
      301 taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa
      361 ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc
      421 gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt
      481 cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca
      541 tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa
      601 tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg
      661 ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac
      721 tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag
      781 tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca
      841 ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac ctgtaggatc
     1021 gtacaggttt acgcaagaaa atggtttgtt atagtcgaat aaatactaga gtcacacagg
     1081 aaagcgcgat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc
     1141 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg
     1201 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat
     1261 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta
     1321 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac
     1381 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg
     1441 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca
     1501 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca
     1561 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag
     1621 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg
     1681 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg
     1741 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg
     1801 ctgcaaacga cgaaaactac gctttagtag cttaataatg cgcatttaca ctttatgctt
     1861 ccggctcgta tggtgtgtgg acctagagaa agaggagaaa tactagatga cagtaaagaa
     1921 gctttatttc gtcccagcag gtcgttgtat gttggatcat tcgtctgtta atagtacatt
     1981 aacaccagga gaattattag acttaccggt ttggtgttat cttttggaga ctgaagaagg
     2041 acctatttta gtagatacag gtatgccaga aagtgcagtt aataatgaag gtctttttaa
     2101 cggtacattt gtcgaagggc aggttttacc gaaaatgact gaagaagata gaatcgtgaa
     2161 tattttaaaa cgggttggtt atgagccgga agaccttctt tatattatta gttctcactt
     2221 gcattttgat catgcaggag gaaatggcgc ttttataaat acaccaatca ttgtacagcg
     2281 tgctgaatat gaggcggcgc agcatagcga agaatatttg aaagaatgta tattgccgaa
     2341 tttaaactac aaaatcattg aaggtgatta tgaagtcgta ccaggagttc aattattgca
     2401 tacaccaggc catactccag ggcatcaatc gctattaatt gagacagaaa aatccggtcc
     2461 tgtattatta acgattgatg catcgtatac gaaagagaat tttgaaaatg aagtgccatt
     2521 tgcgggattt gattcagaat tagctttatc ttcaattaaa cgtttaaaag aagtggtgat
     2581 gaaagagaag ccgattgttt tctttggaca tgatatagag caggaaaggg gatgtaaagt
     2641 gttccctgaa tatatagctg caaacgacga aaactacgct ttagtagctt aataacgctg
     2701 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2761 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2821 actggctcac cttcgggtgg gcctttctgc gtttatacca atgcattggt ttt
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