##gff-version 3
##sequence-region BBa_K1194000 1 1061
BBa_K1194000	.	engineered_region	1007	1061	.	+	0	ID=annotation2359361;Name=BBa_R0062
BBa_K1194000	.	start_codon	1059	1059	.	+	0	ID=annotation2359365;Name=start
BBa_K1194000	.	engineered_region	81	836	.	+	0	ID=annotation2359349;Name=BBa_C0062
BBa_K1194000	.	promoter	1048	1053	.	+	0	ID=annotation2359364;Name=-10
BBa_K1194000	.	point_mutation	254	254	.	+	0	ID=annotation2359351;Name=T
BBa_K1194000	.	engineered_region	63	74	.	+	0	ID=annotation2359346;Name=BBa_B0034
BBa_K1194000	.	promoter	43	48	.	+	0	ID=annotation2359345;Name=-10
BBa_K1194000	.	stem_loop	881	924	.	+	0	ID=annotation2359356;Name=stem_loop
BBa_K1194000	.	engineered_region	958	998	.	+	0	ID=annotation2359357;Name=BBa_B0012
BBa_K1194000	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation2359342;Name=TetR 1
BBa_K1194000	.	point_mutation	572	572	.	+	0	ID=annotation2359352;Name=A
BBa_K1194000	.	engineered_tag	837	861	.	+	0	ID=annotation2359353;Name=Help:Barcodes
BBa_K1194000	.	promoter	20	25	.	+	0	ID=annotation2359343;Name=-35
BBa_K1194000	.	conserved_region	67	70	.	+	0	ID=annotation2359347;Name=conserved
BBa_K1194000	.	engineered_region	870	998	.	+	0	ID=annotation2359355;Name=BBa_B0015
BBa_K1194000	.	engineered_region	1	1061	.	+	0	ID=annotation2359366;Name=BBa_F2620
BBa_K1194000	.	engineered_region	1	54	.	+	0	ID=annotation2359341;Name=BBa_R0040
BBa_K1194000	.	CDS	81	830	.	+	0	ID=annotation2359350;Name=luxR
BBa_K1194000	.	promoter	1026	1031	.	+	0	ID=annotation2359363;Name=-35
BBa_K1194000	.	engineered_region	870	949	.	+	0	ID=annotation2359354;Name=BBa_B0010
BBa_K1194000	.	polyA_site	985	998	.	+	0	ID=annotation2359359;Name=polya
BBa_K1194000	.	stem_loop	965	984	.	+	0	ID=annotation2359358;Name=T7 TE
BBa_K1194000	.	stop_codon	991	991	.	+	0	ID=annotation2359360;Name=stop
BBa_K1194000	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2359362;Name=LuxR/HSL
BBa_K1194000	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation2359344;Name=TetR 2
BBa_K1194000	.	engineered_region	81	82	.	+	0	ID=annotation2359348;Name=prefix
>BBa_K1194000
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaaaaagaggagaaattcagtcatgatgaaacgtaatattctggcagt
tattgttccggcactgctggttgcaggtaccgcaaatgcatggcattggacctggctgagcgaatattaaccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctcacactg
gctcaccttcgggtgggccttctgcgtttata