BBa_K1194001 1 BBa_K1194001 pLuxR --> I3A gene 2013-09-25T11:00:00Z 2015-05-08T01:09:38Z The gene sequence for tryptophan transaminase is derived from Arabidopsis Thaliana. The gene sequence for indole pyruvate decarboxylase is taken from Azospirillum brasilense. The sequence for pLuxR is taken from Registry. The I3A gene is actually a polycistronic construct composed of two genes coding for two enzymes - tryptophan transaminase and indole pyruvate decarboxylase. The two DNA constructs are joined by a linker sequence. This entire assembly is placed downstream of an N-acyl homoserine lactone (AHL) inducible promoter pLuxR. The I3A genes function to convert tryptophan to indole-3-acetaldehyde (I3A). Tryptophan transaminase converts tryptophan to indole pyruvate while indole pyruvate decarboxylase converts indole pyruvate to I3A. I3A is a known biofilm inhibitor. In the future, other teams can use either of our two enzymes as there is a restriction enzyme site incorporated in the linker sequence. The part will be useful in any project involving biosynthesis of indole and its derivatives. false false _1508_ 0 17314 9 It's complicated true Lee JH, Kim YG, Kim CJ, Lee JC, Cho MH, Lee, "Indole-3-acetaldehyde from Rhodococcus sp. BFI 332 inhibits E.coli O157:H7 biofilm formation" Appl Microbiol Biotechnol 2012 Nov:96(4):1071-8 false Aman Kumar annotation2363905 1 BBa_R0062 range2363905 1 1007 1061 annotation2363902 1 T7 TE range2363902 1 965 984 annotation2363888 1 TetR 2 range2363888 1 26 44 annotation2363899 1 BBa_B0015 range2363899 1 870 998 annotation2363910 1 BBa_F2620 range2363910 1 1 1061 annotation2363892 1 prefix range2363892 1 81 82 annotation2363894 1 luxR range2363894 1 81 830 annotation2363890 1 BBa_B0034 range2363890 1 63 74 annotation2363901 1 BBa_B0012 range2363901 1 958 998 annotation2363886 1 TetR 1 range2363886 1 1 19 annotation2363909 1 start range2363909 1 1059 1059 annotation2363911 1 BBa_K1194001 range2363911 1 1070 4113 annotation2363903 1 polya range2363903 1 985 998 annotation2363908 1 -10 range2363908 1 1048 1053 annotation2363907 1 -35 range2363907 1 1026 1031 annotation2363887 1 -35 range2363887 1 20 25 annotation2363889 1 -10 range2363889 1 43 48 annotation2363896 1 A range2363896 1 572 572 annotation2363906 1 LuxR/HSL range2363906 1 1007 1026 annotation2363885 1 BBa_R0040 range2363885 1 1 54 annotation2363900 1 stem_loop range2363900 1 881 924 annotation2363897 1 Help:Barcodes range2363897 1 837 861 annotation2363891 1 conserved range2363891 1 67 70 annotation2363904 1 stop range2363904 1 991 991 annotation2363895 1 T range2363895 1 254 254 annotation2363898 1 BBa_B0010 range2363898 1 870 949 annotation2363893 1 BBa_C0062 range2363893 1 81 836 BBa_K1194001_sequence 1 aaagaggagaaattcagtcatggttaaactggaaaatagccgtaaaccggaaaaaattagcaataaaaatattccgatgagcgattttgttgttaatctggatcatggtgatccgaccgcatatgaagaatattggcgtaaaatgggtgatcgttgtaccgttaccattcgtggttgtgatctgatgagctattttagcgatatgaccaatctgtgttggtttctggaaccggaactggaagatgcaattaaagatctgcatggtgttgttggtaatgcagcaaccgaagatcgttatattgttgttggtaccggtagcacccagctgtgtcaggcagcagttcatgcactgagcagcctggcacgtagccagccggttagcgttgttgcagcagcaccgttttatagcacctatgttgaagaaaccacctatgttcgtagcggtatgtataaatgggaaggtgatgcatggggttttgataaaaaaggtccgtatattgaactggttaccagcccgaataatccggatggtaccattcgtgaaaccgttgttaatcgtccggatgatgatgaagcaaaagttattcatgattttgcatattattggccgcattataccccgattacccgtcgtcaggatcatgatattatgctgtttacctttagcaaaattaccggtcatgcaggtagccgtattggttgggcactggttaaagataaagaagttgcaaaaaaaatggttgaatatattattgttaatagcattggtgttagcaaagaaagccaggttcgtaccgcaaaaattctgaatgttctgaaagaaacctgtaaaagcgaaagcgaaagcgaaaatttttttaaatatggtcgtgaaatgatgaaaaatcgttgggaaaaactgcgtgaagttgttaaagaaagcgatgcatttaccctgccgaaatatccggaagcattttgtaattattttggtaaaagcctggaaagctatccggcatttgcatggctgggtaccaaagaagaaaccgatctggttagcgaactgcgtcgtcataaagttatgagccgtgcaggtgaacgttgtggtagcgataaaaaacatgttcgtgttagcatgctgagccgtgaagatgtttttaatgtttttctggaacgtctggcaaatatgaaactgattaaaagcattgatctgtaaactgaatcaaatttgccacatcaaaaagtctacaaaagaggagaaattcagtcatgaaactggcagaagcactgctgcgtgcactgaaagatcgtggtgcacaggcaatgtttggtattccgggtgattttgcactgccgttttttaaagttgcagaagaaacccagattctgccgctgcataccctgagccatgaaccggcagttggttttgcagcagatgcagcagcacgttatagcagcaccctgggtgttgcagcagttacctatggtgcaggtgcatttaatatggttaatgcagttgcaggtgcatatgcagaaaaaagcccggttgttgttattagcggtgcaccgggtaccaccgaaggtaatgcaggtctgctgctgcatcatcagggtcgtaccctggatacccagtttcaggtttttaaagaaattaccgttgcacaggcacgtctggatgatccggcaaaagcaccggcagaaattgcacgtgttctgggtgcagcacgtgcactgagccgtccggtttatctggaaattccgcgtaatatggttaatgcagaagttgaaccggttggtgatgatccggcatggccggttgatcgtgatgcactggcagcatgtgcagatgaagttctggcagcaatgcgtagcgcaaccagcccggttctgatggtttgtgttgaagttcgtcgttatggtctggaagcaaaagttgcagaactggcacagcgtctgggtgttccggttgttaccacctttatgggtcgtggtctgctggcagatgcaccgaccccgccgctgggtacctatattggtgttgcaggtgatgcagaaattacccgtctggttgaagaaagcgatggtctgtttctgctgggtgcaattctgagcgataccaattttgcagttagccagcgtaaaattgatctgcgtaaaaccattcatgcatttgatcgtgcagttaccctgggttatcatacctatgcagatattccgctggcaggtctggttgatgcactgctggaaggtctgccgccgagcgatcgtaccacccgtggtaaagaaccgcatgcatatccgaccggtctgcaagcagatggtgaaccgattgcaccgatggatattgcacgtgcagttaatgatcgtgttcgtgcaggtcaggaaccgctgctgattgcagcagatatgggtgattgtctgtttaccgcaatggatatgattgatgcaggtctgatggcaccgggttattatgcaggtatgggttttggtgttccggcaggtattggtgcacagtgtgttagcggtggtaaacgtattctgaccgttgttggtgatggtgcatttcagatgaccggttgggaactgggtaattgtcgtcgtctgggtattgatccgattgttattctgtttaataatgcaagctgggaaatgctgcgtacctttcagccggaaagcgcatttaatgatctggatgattggcgttttgcagatatggcagcaggtatgggtggtgatggtgttcgtgttcgtacccgtgcagaactgaaagcagcactggataaagcatttgcaacccgtggtcgttttcagctgattgaagcaatgattccgcgtggtgttctgagcgataccctggcacgttttgttcagggtcagaaacgtctgcatgcagcaccgcgtgaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctcacactggctcaccttcgggtgggccttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z