##gff-version 3
##sequence-region BBa_K1194001 1 4113
BBa_K1194001	.	engineered_region	870	949	.	+	0	ID=annotation2363898;Name=BBa_B0010
BBa_K1194001	.	stem_loop	965	984	.	+	0	ID=annotation2363902;Name=T7 TE
BBa_K1194001	.	engineered_region	1007	1061	.	+	0	ID=annotation2363905;Name=BBa_R0062
BBa_K1194001	.	engineered_region	63	74	.	+	0	ID=annotation2363890;Name=BBa_B0034
BBa_K1194001	.	engineered_region	1	1061	.	+	0	ID=annotation2363910;Name=BBa_F2620
BBa_K1194001	.	CDS	81	830	.	+	0	ID=annotation2363894;Name=luxR
BBa_K1194001	.	promoter	1048	1053	.	+	0	ID=annotation2363908;Name=-10
BBa_K1194001	.	stem_loop	881	924	.	+	0	ID=annotation2363900;Name=stem_loop
BBa_K1194001	.	promoter	1026	1031	.	+	0	ID=annotation2363907;Name=-35
BBa_K1194001	.	start_codon	1059	1059	.	+	0	ID=annotation2363909;Name=start
BBa_K1194001	.	engineered_region	958	998	.	+	0	ID=annotation2363901;Name=BBa_B0012
BBa_K1194001	.	point_mutation	572	572	.	+	0	ID=annotation2363896;Name=A
BBa_K1194001	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation2363886;Name=TetR 1
BBa_K1194001	.	engineered_region	1	54	.	+	0	ID=annotation2363885;Name=BBa_R0040
BBa_K1194001	.	promoter	43	48	.	+	0	ID=annotation2363889;Name=-10
BBa_K1194001	.	polyA_site	985	998	.	+	0	ID=annotation2363903;Name=polya
BBa_K1194001	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2363906;Name=LuxR/HSL
BBa_K1194001	.	engineered_region	1070	4113	.	+	0	ID=annotation2363911;Name=BBa_K1194001
BBa_K1194001	.	engineered_region	870	998	.	+	0	ID=annotation2363899;Name=BBa_B0015
BBa_K1194001	.	engineered_tag	837	861	.	+	0	ID=annotation2363897;Name=Help:Barcodes
BBa_K1194001	.	conserved_region	67	70	.	+	0	ID=annotation2363891;Name=conserved
BBa_K1194001	.	engineered_region	81	82	.	+	0	ID=annotation2363892;Name=prefix
BBa_K1194001	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation2363888;Name=TetR 2
BBa_K1194001	.	stop_codon	991	991	.	+	0	ID=annotation2363904;Name=stop
BBa_K1194001	.	promoter	20	25	.	+	0	ID=annotation2363887;Name=-35
BBa_K1194001	.	point_mutation	254	254	.	+	0	ID=annotation2363895;Name=T
BBa_K1194001	.	engineered_region	81	836	.	+	0	ID=annotation2363893;Name=BBa_C0062
>BBa_K1194001
aaagaggagaaattcagtcatggttaaactggaaaatagccgtaaaccggaaaaaattagcaataaaaatattccgatg
agcgattttgttgttaatctggatcatggtgatccgaccgcatatgaagaatattggcgtaaaatgggtgatcgttgta
ccgttaccattcgtggttgtgatctgatgagctattttagcgatatgaccaatctgtgttggtttctggaaccggaact
ggaagatgcaattaaagatctgcatggtgttgttggtaatgcagcaaccgaagatcgttatattgttgttggtaccggt
agcacccagctgtgtcaggcagcagttcatgcactgagcagcctggcacgtagccagccggttagcgttgttgcagcag
caccgttttatagcacctatgttgaagaaaccacctatgttcgtagcggtatgtataaatgggaaggtgatgcatgggg
ttttgataaaaaaggtccgtatattgaactggttaccagcccgaataatccggatggtaccattcgtgaaaccgttgtt
aatcgtccggatgatgatgaagcaaaagttattcatgattttgcatattattggccgcattataccccgattacccgtc
gtcaggatcatgatattatgctgtttacctttagcaaaattaccggtcatgcaggtagccgtattggttgggcactggt
taaagataaagaagttgcaaaaaaaatggttgaatatattattgttaatagcattggtgttagcaaagaaagccaggtt
cgtaccgcaaaaattctgaatgttctgaaagaaacctgtaaaagcgaaagcgaaagcgaaaatttttttaaatatggtc
gtgaaatgatgaaaaatcgttgggaaaaactgcgtgaagttgttaaagaaagcgatgcatttaccctgccgaaatatcc
ggaagcattttgtaattattttggtaaaagcctggaaagctatccggcatttgcatggctgggtaccaaagaagaaacc
gatctggttagcgaactgcgtcgtcataaagttatgagccgtgcaggtgaacgttgtggtagcgataaaaaacatgttc
gtgttagcatgctgagccgtgaagatgtttttaatgtttttctggaacgtctggcaaatatgaaactgattaaaagcat
tgatctgtaaactgaatcaaatttgccacatcaaaaagtctacaaaagaggagaaattcagtcatgaaactggcagaag
cactgctgcgtgcactgaaagatcgtggtgcacaggcaatgtttggtattccgggtgattttgcactgccgttttttaa
agttgcagaagaaacccagattctgccgctgcataccctgagccatgaaccggcagttggttttgcagcagatgcagca
gcacgttatagcagcaccctgggtgttgcagcagttacctatggtgcaggtgcatttaatatggttaatgcagttgcag
gtgcatatgcagaaaaaagcccggttgttgttattagcggtgcaccgggtaccaccgaaggtaatgcaggtctgctgct
gcatcatcagggtcgtaccctggatacccagtttcaggtttttaaagaaattaccgttgcacaggcacgtctggatgat
ccggcaaaagcaccggcagaaattgcacgtgttctgggtgcagcacgtgcactgagccgtccggtttatctggaaattc
cgcgtaatatggttaatgcagaagttgaaccggttggtgatgatccggcatggccggttgatcgtgatgcactggcagc
atgtgcagatgaagttctggcagcaatgcgtagcgcaaccagcccggttctgatggtttgtgttgaagttcgtcgttat
ggtctggaagcaaaagttgcagaactggcacagcgtctgggtgttccggttgttaccacctttatgggtcgtggtctgc
tggcagatgcaccgaccccgccgctgggtacctatattggtgttgcaggtgatgcagaaattacccgtctggttgaaga
aagcgatggtctgtttctgctgggtgcaattctgagcgataccaattttgcagttagccagcgtaaaattgatctgcgt
aaaaccattcatgcatttgatcgtgcagttaccctgggttatcatacctatgcagatattccgctggcaggtctggttg
atgcactgctggaaggtctgccgccgagcgatcgtaccacccgtggtaaagaaccgcatgcatatccgaccggtctgca
agcagatggtgaaccgattgcaccgatggatattgcacgtgcagttaatgatcgtgttcgtgcaggtcaggaaccgctg
ctgattgcagcagatatgggtgattgtctgtttaccgcaatggatatgattgatgcaggtctgatggcaccgggttatt
atgcaggtatgggttttggtgttccggcaggtattggtgcacagtgtgttagcggtggtaaacgtattctgaccgttgt
tggtgatggtgcatttcagatgaccggttgggaactgggtaattgtcgtcgtctgggtattgatccgattgttattctg
tttaataatgcaagctgggaaatgctgcgtacctttcagccggaaagcgcatttaatgatctggatgattggcgttttg
cagatatggcagcaggtatgggtggtgatggtgttcgtgttcgtacccgtgcagaactgaaagcagcactggataaagc
atttgcaacccgtggtcgttttcagctgattgaagcaatgattccgcgtggtgttctgagcgataccctggcacgtttt
gttcagggtcagaaacgtctgcatgcagcaccgcgtgaataaccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctcacactggctcaccttcgggtgggccttctgcgtt
tata