BBa_K1196008
type: ComponentDefinition
persistent identity: BBa K1196008
display id: BBa_K1196008
version: 1
was generated by: iGEM to SBOL conversion
description: hydrazine reductase and luciferase
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:38Z
mutable provenance: MYSELF
owned by: james
owned by: myers
mutable description: This device can be used to synthesize hydrazine reductase and luciferase.
discontinued: false
dominant: false
group u list: _1510_
m user id: 0
owner id: 18361
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This device can be used to synthesize hydrazine reductase and luciferase.
star: false
creator: Sheng Ding
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1196007
component: BBa_K1196006
sequence annotation: BBa_K1196006
sequence annotation: BBa_K1196007
sequence: https://synbiohub.org/public/igem/BBa_K1196008_sequence/1

BBa_K1196007
type: Component
persistent identity: component2333616
display id: component2333616
version: 1
top level: BBa_K1196008
definition: BBa_K1196007
access: public

BBa_K1196006
type: Component
persistent identity: component2333599
display id: component2333599
version: 1
top level: BBa_K1196008
definition: BBa_K1196006
access: public

BBa_K1196006
type: SequenceAnnotation
persistent identity: annotation2333599
display id: annotation2333599
version: 1
top level: BBa_K1196008
location: https://synbiohub.org/public/igem/BBa_K1196008/annotation2333599/range2333599/1
component: BBa_K1196006

https://synbiohub.org/public/igem/BBa_K1196008/annotation2333599/range2333599/1
type: Range
persistent identity: range2333599
display id: range2333599
version: 1
top level: BBa_K1196008
direction: direction/reverse
start: 1
end: 764
orientation: inline

BBa_K1196007
type: SequenceAnnotation
persistent identity: annotation2333616
display id: annotation2333616
version: 1
top level: BBa_K1196008
location: https://synbiohub.org/public/igem/BBa_K1196008/annotation2333616/range2333616/1
component: BBa_K1196007

https://synbiohub.org/public/igem/BBa_K1196008/annotation2333616/range2333616/1
type: Range
persistent identity: range2333616
display id: range2333616
version: 1
top level: BBa_K1196008
direction: direction/reverse
start: 773
end: 1746
orientation: inline

BBa_K1196006
type: ComponentDefinition
persistent identity: BBa K1196006
display id: BBa_K1196006
version: 1
was generated by: iGEM to SBOL conversion
description: hydrazine reductase device
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:38Z
mutable provenance: myself
owned by: james
owned by: myers
mutable description: We hope it can be used in the synthesis of hydrazine reductase.
discontinued: false
dominant: false
experience: experience/None
group u list: _1510_
m user id: 0
owner id: 18361
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We hope it can be used in the synthesis of hydrazine reductase.
star: false
creator: Sheng Ding
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_R0063
component: BBa_B0010
component: BBa_K1196004
component: BBa_B0012
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_K1196004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1196006_sequence/1

BBa_B0034
type: Component
persistent identity: component2333560
display id: component2333560
version: 1
top level: BBa_K1196006
definition: BBa_B0034
access: public

BBa_R0063
type: Component
persistent identity: component2333554
display id: component2333554
version: 1
top level: BBa_K1196006
definition: lux pL
access: public

BBa_B0010
type: Component
persistent identity: component2333562
display id: component2333562
version: 1
top level: BBa_K1196006
definition: BBa_B0010
access: public

BBa_K1196004
type: Component
persistent identity: component2333561
display id: component2333561
version: 1
top level: BBa_K1196006
definition: BBa_K1196004
access: public

BBa_B0012
type: Component
persistent identity: component2333564
display id: component2333564
version: 1
top level: BBa_K1196006
definition: BBa_B0012
access: public

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2333554
display id: annotation2333554
version: 1
top level: BBa_K1196006
location: https://synbiohub.org/public/igem/BBa_K1196006/annotation2333554/range2333554/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K1196006/annotation2333554/range2333554/1
type: Range
persistent identity: range2333554
display id: range2333554
version: 1
top level: BBa_K1196006
direction: direction/forward
start: 1
end: 151
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2333560
display id: annotation2333560
version: 1
top level: BBa_K1196006
location: https://synbiohub.org/public/igem/BBa_K1196006/annotation2333560/range2333560/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1196006/annotation2333560/range2333560/1
type: Range
persistent identity: range2333560
display id: range2333560
version: 1
top level: BBa_K1196006
direction: direction/reverse
start: 160
end: 171
orientation: inline

BBa_K1196004
type: SequenceAnnotation
persistent identity: annotation2333561
display id: annotation2333561
version: 1
top level: BBa_K1196006
location: https://synbiohub.org/public/igem/BBa_K1196006/annotation2333561/range2333561/1
component: BBa_K1196004

https://synbiohub.org/public/igem/BBa_K1196006/annotation2333561/range2333561/1
type: Range
persistent identity: range2333561
display id: range2333561
version: 1
top level: BBa_K1196006
direction: direction/reverse
start: 178
end: 627
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2333562
display id: annotation2333562
version: 1
top level: BBa_K1196006
location: https://synbiohub.org/public/igem/BBa_K1196006/annotation2333562/range2333562/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1196006/annotation2333562/range2333562/1
type: Range
persistent identity: range2333562
display id: range2333562
version: 1
top level: BBa_K1196006
direction: direction/forward
start: 636
end: 715
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2333564
display id: annotation2333564
version: 1
top level: BBa_K1196006
location: https://synbiohub.org/public/igem/BBa_K1196006/annotation2333564/range2333564/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1196006/annotation2333564/range2333564/1
type: Range
persistent identity: range2333564
display id: range2333564
version: 1
top level: BBa_K1196006
direction: direction/forward
start: 724
end: 764
orientation: inline

BBa_K1196007
type: ComponentDefinition
persistent identity: BBa K1196007
display id: BBa_K1196007
version: 1
was generated by: iGEM to SBOL conversion
description: luciferase
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:38Z
mutable provenance: Run-ze Zhang
owned by: james
owned by: myers
mutable description: This part can be used to synthetize a luciferase .
discontinued: false
dominant: false
group u list: _1510_
m user id: 0
owner id: 18362
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part can be used to synthetize a luciferase .
star: false
creator: Run-ze Zhang
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0051
component: BBa_E0034
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_E0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1196007_sequence/1

BBa_R0051
type: Component
persistent identity: component2333569
display id: component2333569
version: 1
top level: BBa_K1196007
definition: cI lam
access: public

BBa_E0034
type: Component
persistent identity: component2333577
display id: component2333577
version: 1
top level: BBa_K1196007
definition: EYFP
access: public

BBa_B0034
type: Component
persistent identity: component2333574
display id: component2333574
version: 1
top level: BBa_K1196007
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2333580
display id: component2333580
version: 1
top level: BBa_K1196007
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2333578
display id: component2333578
version: 1
top level: BBa_K1196007
definition: BBa_B0010
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2333569
display id: annotation2333569
version: 1
top level: BBa_K1196007
location: https://synbiohub.org/public/igem/BBa_K1196007/annotation2333569/range2333569/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1196007/annotation2333569/range2333569/1
type: Range
persistent identity: range2333569
display id: range2333569
version: 1
top level: BBa_K1196007
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2333574
display id: annotation2333574
version: 1
top level: BBa_K1196007
location: https://synbiohub.org/public/igem/BBa_K1196007/annotation2333574/range2333574/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1196007/annotation2333574/range2333574/1
type: Range
persistent identity: range2333574
display id: range2333574
version: 1
top level: BBa_K1196007
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation2333577
display id: annotation2333577
version: 1
top level: BBa_K1196007
location: https://synbiohub.org/public/igem/BBa_K1196007/annotation2333577/range2333577/1
component: BBa_E0034

https://synbiohub.org/public/igem/BBa_K1196007/annotation2333577/range2333577/1
type: Range
persistent identity: range2333577
display id: range2333577
version: 1
top level: BBa_K1196007
direction: direction/reverse
start: 76
end: 837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2333578
display id: annotation2333578
version: 1
top level: BBa_K1196007
location: https://synbiohub.org/public/igem/BBa_K1196007/annotation2333578/range2333578/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1196007/annotation2333578/range2333578/1
type: Range
persistent identity: range2333578
display id: range2333578
version: 1
top level: BBa_K1196007
direction: direction/forward
start: 846
end: 925
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2333580
display id: annotation2333580
version: 1
top level: BBa_K1196007
location: https://synbiohub.org/public/igem/BBa_K1196007/annotation2333580/range2333580/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1196007/annotation2333580/range2333580/1
type: Range
persistent identity: range2333580
display id: range2333580
version: 1
top level: BBa_K1196007
direction: direction/forward
start: 934
end: 974
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pL)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
top level: lux pL
direction: direction/forward
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
top level: lux pL
direction: direction/forward
start: 115
end: 122
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
top level: lux pL
direction: direction/forward
start: 128
end: 128
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
top level: lux pL
direction: direction/forward
start: 130
end: 149
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: AAV
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
top level: EYFP
direction: direction/forward
start: 718
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
top level: EYFP
direction: direction/forward
start: 757
end: 762
orientation: inline

BBa_K1196004
type: ComponentDefinition
persistent identity: BBa K1196004
display id: BBa_K1196004
version: 1
was generated by: iGEM to SBOL conversion
description: Hydrazine oxidoreductase
created: 2013-08-25T11:00:00Z
modified: 2015-05-08T01:09:38Z
mutable provenance: "Community structures of anammox bacteria and their similar distribution with nirS-encoding nitrite-reducing bacteria in surface sediment of the South China Sea." Li M., Hong Y., Cao H., Gu J. Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
owned by: james
owned by: myers
mutable description: Reaction(IUBMB) hydrazine + acceptor = N2 + reduced acceptor The enzyme is involved in the pathway of anaerobic ammonium oxidation in anammox bacteria. The conversion of hydroxylamine by the enzyme from Brocadia anammoxidans results in the formation of NO and N2O [1]. Hydroxylamine is not a substrate for the enzyme from planctomycete KSU-1
discontinued: false
dominant: false
group u list: _1510_
m user id: 0
owner id: 18361
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Reaction(IUBMB) hydrazine + acceptor = N2 + reduced acceptor
star: false
creator: Sheng Ding
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1196004_sequence/1

https://synbiohub.org/public/igem/BBa_K1196008_sequence/1
type: Sequence
persistent identity: BBa K1196008 sequence
display id: BBa_K1196008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgtcaaatgcagacagaggtgcgcctctctggaaggaaaagagagacacctgggtatcagtatgtgacgattgccattcaccaaggtttgcaagagagaacttgcaggcgatggacgaagcttgtaaggatgcaggtctgaagtatactgaaacgtttaaagtagcagagaatttgatgcttgatggtatgggcgagcctatgcctaaggatcttcatcctgactggagtggtcagcacatctggagtttgaagattggtgcttatcatgatggaccgaagtatggtggtaagaagggtgagtccggtgagttcagaatgtctaactgttcagacatagaaagagtatgttttgagagcgttggatactggatgacttacatattcaagggaatggcgcatggttcatggaacgatgcgacatattgtgacgggtcgtttggtatggactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1196006_sequence/1
type: Sequence
persistent identity: BBa K1196006 sequence
display id: BBa_K1196006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgtcaaatgcagacagaggtgcgcctctctggaaggaaaagagagacacctgggtatcagtatgtgacgattgccattcaccaaggtttgcaagagagaacttgcaggcgatggacgaagcttgtaaggatgcaggtctgaagtatactgaaacgtttaaagtagcagagaatttgatgcttgatggtatgggcgagcctatgcctaaggatcttcatcctgactggagtggtcagcacatctggagtttgaagattggtgcttatcatgatggaccgaagtatggtggtaagaagggtgagtccggtgagttcagaatgtctaactgttcagacatagaaagagtatgttttgagagcgttggatactggatgacttacatattcaagggaatggcgcatggttcatggaacgatgcgacatattgtgacgggtcgtttggtatggactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1196007_sequence/1
type: Sequence
persistent identity: BBa K1196007 sequence
display id: BBa_K1196007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0063_sequence/1
type: Sequence
persistent identity: BBa R0063 sequence
display id: BBa_R0063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0034_sequence/1
type: Sequence
persistent identity: BBa E0034 sequence
display id: BBa_E0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1196004_sequence/1
type: Sequence
persistent identity: BBa K1196004 sequence
display id: BBa_K1196004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcaaatgcagacagaggtgcgcctctctggaaggaaaagagagacacctgggtatcagtatgtgacgattgccattcaccaaggtttgcaagagagaacttgcaggcgatggacgaagcttgtaaggatgcaggtctgaagtatactgaaacgtttaaagtagcagagaatttgatgcttgatggtatgggcgagcctatgcctaaggatcttcatcctgactggagtggtcagcacatctggagtttgaagattggtgcttatcatgatggaccgaagtatggtggtaagaagggtgagtccggtgagttcagaatgtctaactgttcagacatagaaagagtatgttttgagagcgttggatactggatgacttacatattcaagggaatggcgcatggttcatggaacgatgcgacatattgtgacgggtcgtttggtatggac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z