BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K1196018
1
BBa_K1196018
nitrite reductase device
2013-09-15T11:00:00Z
2015-05-08T01:09:39Z
anammox
This part create nitrite reductase.
false
false
_1510_
0
18361
9
Not in stock
false
This part create nitrite reductase.
false
Sheng Ding
component2346829
1
BBa_B0030
component2346832
1
BBa_B0010
component2346827
1
BBa_J23100
component2346831
1
BBa_K1196009
component2346834
1
BBa_B0012
annotation2346832
1
BBa_B0010
range2346832
1
1233
1312
annotation2346829
1
BBa_B0030
range2346829
1
44
58
annotation2346827
1
BBa_J23100
range2346827
1
1
35
annotation2346834
1
BBa_B0012
range2346834
1
1321
1361
annotation2346831
1
BBa_K1196009
range2346831
1
67
1224
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K1196009
1
BBa_K1196009
nitrite reductase
2013-09-09T11:00:00Z
2015-05-08T01:09:38Z
AUTHORS Yu,G.H., Chen,Y.H., Li,Y.J., Yang,Z.H. and Chen,Y.F.
TITLE Cloning and sequence analysis of nitrite reductase from the
nitrite-degrading strain 2-8
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1158)
AUTHORS Chen,Y.H., Yu,G.H., Li,Y.J., Chen,Y.F. and Yang,Z.H.
TITLE Direct Submission
JOURNAL Submitted (17-DEC-2009) Microbiology, Zhuhai Agricultural Sciences
Research Center, Meixi Qianshan of Zhuhai City, Zhuhai, Guangdong
519070, China
nitrite reductase for Anammox
Nitric oxide + H2O + Ferricytochrome c <=> Nitrite + Ferrocytochrome c + H+
false
false
_1510_
0
18362
9
Not in stock
false
Nitric oxide + H2O + Ferricytochrome c <=> Nitrite + Ferrocytochrome c + H+
false
Run-ze Zhang
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1196018_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagaggcaaggacctctactatgatgacccgtcgagacactctgttgggcgccgccgcgaccgctctgctgctcagcgccgtgccgaacgtggcgttcgccgccgaggacgaaatctctaaactgccgcgccagaagattgagctggtggcgccgccgttcgtgcacgcgcatcagcaggccaccaaggacggcccgaaggtcgtcgagttcaagctggttgtccaggaaaaggaagtggtgatcgacgaggacggcaccaaattccaggcgatgacattcaacggctcgatgcccggaccgttgatggtggtgcacgagggcgactacgtcgagctgacgctggtcaatcccgccaccaacaccatgccacacaatatcgacttccacgcctcgaccggcgcgctcggcggcggcgcgctgaccttgatcaatcccggcgaacaggtggtgctgcggttcaaggccgaccgcgccggcaccttcgtgtatcactgcgcaccgggcggcaacatgatcccgtggcacgtcgtttccggcatgaacggcgccatgatggtgctgccgcgcgacggcctgaaggatcggaacggcaagccgctgcactacgaccgcatctattacatcggcgagcaggacatgtacgtgccgaaggacgagcacggcaaatacaaatccttcgcctcaccggccgagtcctatgtcgacacggtcgaggtgatgcgcaagctgatcccgacccacgtggtattcaacggcaaggtgggcgcgctgaccggcaaggacgcgatgaccgccaaggtcggcgagaccgtgttgatcgtgcattcgcaggccaaccgcgacacccgcccgcacatcatcggcggccacggcgattatgtctgggagaccggcaagttcgccaatccgccggagaccaatctggagacctggttcatccgcggcggctcggccggagctgcgctgtacaccttcaagcagccgggcgtgtacgcctacgtcaaccacaacctgatcgaagcggtcgaactcggcgccaccgcgcacttcaaggtcgacggcaagtggaacgacgacctgatgaagcaggtcagcgcccccggcccgatcccgggcggcccgaccaactaagactccgcgcttccgaggctcacgtttgtgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1196009_sequence
1
gcaaggacctctactatgatgacccgtcgagacactctgttgggcgccgccgcgaccgctctgctgctcagcgccgtgccgaacgtggcgttcgccgccgaggacgaaatctctaaactgccgcgccagaagattgagctggtggcgccgccgttcgtgcacgcgcatcagcaggccaccaaggacggcccgaaggtcgtcgagttcaagctggttgtccaggaaaaggaagtggtgatcgacgaggacggcaccaaattccaggcgatgacattcaacggctcgatgcccggaccgttgatggtggtgcacgagggcgactacgtcgagctgacgctggtcaatcccgccaccaacaccatgccacacaatatcgacttccacgcctcgaccggcgcgctcggcggcggcgcgctgaccttgatcaatcccggcgaacaggtggtgctgcggttcaaggccgaccgcgccggcaccttcgtgtatcactgcgcaccgggcggcaacatgatcccgtggcacgtcgtttccggcatgaacggcgccatgatggtgctgccgcgcgacggcctgaaggatcggaacggcaagccgctgcactacgaccgcatctattacatcggcgagcaggacatgtacgtgccgaaggacgagcacggcaaatacaaatccttcgcctcaccggccgagtcctatgtcgacacggtcgaggtgatgcgcaagctgatcccgacccacgtggtattcaacggcaaggtgggcgcgctgaccggcaaggacgcgatgaccgccaaggtcggcgagaccgtgttgatcgtgcattcgcaggccaaccgcgacacccgcccgcacatcatcggcggccacggcgattatgtctgggagaccggcaagttcgccaatccgccggagaccaatctggagacctggttcatccgcggcggctcggccggagctgcgctgtacaccttcaagcagccgggcgtgtacgcctacgtcaaccacaacctgatcgaagcggtcgaactcggcgccaccgcgcacttcaaggtcgacggcaagtggaacgacgacctgatgaagcaggtcagcgcccccggcccgatcccgggcggcccgaccaactaagactccgcgcttccgaggctcacgtttgtg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z