BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23102
1
BBa_J23102
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
isolated from library of promoters
Released HQ 2013
replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1196014
1
BBa_K1196014
formate dehydrogenase
2013-09-09T11:00:00Z
2015-05-08T01:09:38Z
Authors DAVISON DC.
Title Studies on plant formic dehydrogenase.
Journal Biochem. J. 49 (1951) 520-6.
Organism Phaseolus vulgaris
Oxidoreductases;Acting on the aldehyde or oxo group of donors;With NAD+ or NADP+ as acceptor.
The enzyme from most aerobic organisms is devoid of redox-active centres but that from the proteobacterium Methylosinus trichosporium contains iron-sulfur centres, flavin and a molybdenum centre [3]. Together with EC 1.12.1.2 hydrogen dehydrogenase, forms a system previously known as formate hydrogenlyase.
false
false
_1510_
0
18792
9
Not in stock
false
Oxidoreductases;Acting on the aldehyde or oxo group of donors;With NAD+ or NADP+ as acceptor.
The enzyme from most aerobic organisms is devoid of redox-active centres but that from the proteobacterium Methylosinus trichosporium contains iron-sulfur centres, flavin and a molybdenum centre [3]. Together with EC 1.12.1.2 hydrogen dehydrogenase, forms a system previously known as formate hydrogenlyase.
false
Tao Sun
BBa_K1196021
1
BBa_K1196021
Formate dehydrogenase device
2013-09-15T11:00:00Z
2015-05-08T01:09:39Z
anammox
This part synthesize formate dehydrogenase.
false
false
_1510_
0
18361
9
Not in stock
false
This part synthesize formate dehydrogenase.
false
Sheng Ding
component2346889
1
BBa_B0032
component2346894
1
BBa_B0012
component2346892
1
BBa_B0010
component2346887
1
BBa_J23102
component2346891
1
BBa_K1196014
annotation2346891
1
BBa_K1196014
range2346891
1
65
407
annotation2346887
1
BBa_J23102
range2346887
1
1
35
annotation2346892
1
BBa_B0010
range2346892
1
416
495
annotation2346889
1
BBa_B0032
range2346889
1
44
56
annotation2346894
1
BBa_B0012
range2346894
1
504
544
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0032_sequence
1
tcacacaggaaag
BBa_J23102_sequence
1
ttgacagctagctcagtcctaggtactgtgctagc
BBa_K1196021_sequence
1
ttgacagctagctcagtcctaggtactgtgctagctactagagtcacacaggaaagtactagagctcctatagggcgaattgggccctgcatgctcgagcggccgccagtgtgatggatataattcgcccttcattccaaaatgttgtttgacattgatacaggctagagaggaggcctaaatgtgagtaaagtttgtctttcaccatacatagttttcactgagtaaagcacgatggaacaacttgaatgtgtcttcattgaaactgctgctctgaggctacagaatgccagaacggtagaaaaaacgatttccggtttcaggggacgatatcttagcaaggccagtgtttgaagagggtgccaaaacagtgaaagttcatttaccgggtaaagacgagtattggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1196014_sequence
1
ctcctatagggcgaattgggccctgcatgctcgagcggccgccagtgtgatggatataattcgcccttcattccaaaatgttgtttgacattgatacaggctagagaggaggcctaaatgtgagtaaagtttgtctttcaccatacatagttttcactgagtaaagcacgatggaacaacttgaatgtgtcttcattgaaactgctgctctgaggctacagaatgccagaacggtagaaaaaacgatttccggtttcaggggacgatatcttagcaaggccagtgtttgaagagggtgccaaaacagtgaaagttcatttaccgggtaaagacgagtattggt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z