LOCUS BBa_K1196024 5047 bp DNA linear 14-Nov-2024 DEFINITION Our main part ACCESSION BBa_K1196024 VERSION BBa_K1196024.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 CDS 67..1224 /label=BBa_K1196009 stem_loop 1244..1287 /label=stem_loop misc_feature 1233..1312 /label=BBa_B0010 terminator 1233..1312 /label=BBa_B0010 stem_loop 1328..1347 /label=T7 TE misc_feature 1354..1354 /label=stop polyA_site 1348..1361 /label=polya misc_feature 1321..1361 /label=BBa_B0012 terminator 1321..1361 /label=BBa_B0012 misc_feature 1..1361 /label=BBa_K1196018 promoter 1370..1404 /label=BBa_J23100 RBS 1413..1427 /label=RBS-1\Strong RBS 1420..1424 /label=RBS misc_feature 1413..1427 /label=BBa_B0030 RBS 1413..1427 /label=BBa_B0030 CDS 1436..1630 /label=BBa_K1196011 stem_loop 1650..1693 /label=stem_loop misc_feature 1639..1718 /label=BBa_B0010 terminator 1639..1718 /label=BBa_B0010 stem_loop 1734..1753 /label=T7 TE misc_feature 1760..1760 /label=stop polyA_site 1754..1767 /label=polya misc_feature 1727..1767 /label=BBa_B0012 terminator 1727..1767 /label=BBa_B0012 misc_feature 1370..1767 /label=BBa_K1196019 promoter 1776..1810 /label=BBa_J23101 misc_feature 1825..1828 /label=conserved RBS 1819..1832 /label=BBa_B0031 CDS 1839..2288 /label=BBa_K1196004 stem_loop 2308..2351 /label=stem_loop misc_feature 2297..2376 /label=BBa_B0010 terminator 2297..2376 /label=BBa_B0010 stem_loop 2392..2411 /label=T7 TE misc_feature 2418..2418 /label=stop polyA_site 2412..2425 /label=polya misc_feature 2385..2425 /label=BBa_B0012 terminator 2385..2425 /label=BBa_B0012 misc_feature 1776..2425 /label=BBa_K1196020 promoter 2434..2468 /label=BBa_J23102 RBS 2477..2489 /label=RBS-3\Weak RBS 2483..2486 /label=RBS misc_feature 2477..2489 /label=BBa_B0032 RBS 2477..2489 /label=BBa_B0032 CDS 2498..2840 /label=BBa_K1196014 stem_loop 2860..2903 /label=stem_loop misc_feature 2849..2928 /label=BBa_B0010 terminator 2849..2928 /label=BBa_B0010 stem_loop 2944..2963 /label=T7 TE misc_feature 2970..2970 /label=stop polyA_site 2964..2977 /label=polya misc_feature 2937..2977 /label=BBa_B0012 terminator 2937..2977 /label=BBa_B0012 misc_feature 2434..2977 /label=BBa_K1196021 promoter 2986..3020 /label=BBa_J23100 RBS 3029..3043 /label=RBS-1\Strong RBS 3036..3040 /label=RBS misc_feature 3029..3043 /label=BBa_B0030 RBS 3029..3043 /label=BBa_B0030 CDS 3052..3888 /label=BBa_K1196013 stem_loop 3908..3951 /label=stem_loop misc_feature 3897..3976 /label=BBa_B0010 terminator 3897..3976 /label=BBa_B0010 stem_loop 3992..4011 /label=T7 TE misc_feature 4018..4018 /label=stop polyA_site 4012..4025 /label=polya misc_feature 3985..4025 /label=BBa_B0012 terminator 3985..4025 /label=BBa_B0012 misc_feature 2986..4025 /label=BBa_K1196022 promoter 4034..4068 /label=BBa_J23100 RBS 4077..4091 /label=RBS-1\Strong RBS 4084..4088 /label=RBS misc_feature 4077..4091 /label=BBa_B0030 RBS 4077..4091 /label=BBa_B0030 CDS 4098..4910 /label=BBa_K1196015 stem_loop 4930..4973 /label=stem_loop misc_feature 4919..4998 /label=BBa_B0010 terminator 4919..4998 /label=BBa_B0010 stem_loop 5014..5033 /label=T7 TE misc_feature 5040..5040 /label=stop polyA_site 5034..5047 /label=polya misc_feature 5007..5047 /label=BBa_B0012 terminator 5007..5047 /label=BBa_B0012 misc_feature 4034..5047 /label=BBa_K1196023 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata 61 ctagaggcaa ggacctctac tatgatgacc cgtcgagaca ctctgttggg cgccgccgcg 121 accgctctgc tgctcagcgc cgtgccgaac gtggcgttcg ccgccgagga cgaaatctct 181 aaactgccgc gccagaagat tgagctggtg gcgccgccgt tcgtgcacgc gcatcagcag 241 gccaccaagg acggcccgaa ggtcgtcgag ttcaagctgg ttgtccagga aaaggaagtg 301 gtgatcgacg aggacggcac caaattccag gcgatgacat tcaacggctc gatgcccgga 361 ccgttgatgg tggtgcacga gggcgactac gtcgagctga cgctggtcaa tcccgccacc 421 aacaccatgc cacacaatat cgacttccac gcctcgaccg gcgcgctcgg cggcggcgcg 481 ctgaccttga tcaatcccgg cgaacaggtg gtgctgcggt tcaaggccga ccgcgccggc 541 accttcgtgt atcactgcgc accgggcggc aacatgatcc cgtggcacgt cgtttccggc 601 atgaacggcg ccatgatggt gctgccgcgc gacggcctga aggatcggaa cggcaagccg 661 ctgcactacg accgcatcta ttacatcggc gagcaggaca tgtacgtgcc gaaggacgag 721 cacggcaaat acaaatcctt cgcctcaccg gccgagtcct atgtcgacac ggtcgaggtg 781 atgcgcaagc tgatcccgac ccacgtggta ttcaacggca aggtgggcgc gctgaccggc 841 aaggacgcga tgaccgccaa ggtcggcgag accgtgttga tcgtgcattc gcaggccaac 901 cgcgacaccc gcccgcacat catcggcggc cacggcgatt atgtctggga gaccggcaag 961 ttcgccaatc cgccggagac caatctggag acctggttca tccgcggcgg ctcggccgga 1021 gctgcgctgt acaccttcaa gcagccgggc gtgtacgcct acgtcaacca caacctgatc 1081 gaagcggtcg aactcggcgc caccgcgcac ttcaaggtcg acggcaagtg gaacgacgac 1141 ctgatgaagc aggtcagcgc ccccggcccg atcccgggcg gcccgaccaa ctaagactcc 1201 gcgcttccga ggctcacgtt tgtgtactag agccaggcat caaataaaac gaaaggctca 1261 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1321 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacggctag 1381 ctcagtccta ggtacagtgc tagctactag agattaaaga ggagaaatac tagaggggta 1441 tcagtatgta gaggatgatg gttcaaccgt tacctcacag ttggcagacg taccgtatta 1501 catccagatt ctggatgaca agggtatgtc cgtacagacg ggtttatcct gggcatactt 1561 aaggccgtat catggtagga ttggatgtca ctatggatca tatcgtggaa gggcatttaa 1621 gaatttgcat tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1681 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1741 cttcgggtgg gcctttctgc gtttatatac tagagtttac agctagctca gtcctaggta 1801 ttatgctagc tactagagtc acacaggaaa cctactagat gtcaaatgca gacagaggtg 1861 cgcctctctg gaaggaaaag agagacacct gggtatcagt atgtgacgat tgccattcac 1921 caaggtttgc aagagagaac ttgcaggcga tggacgaagc ttgtaaggat gcaggtctga 1981 agtatactga aacgtttaaa gtagcagaga atttgatgct tgatggtatg ggcgagccta 2041 tgcctaagga tcttcatcct gactggagtg gtcagcacat ctggagtttg aagattggtg 2101 cttatcatga tggaccgaag tatggtggta agaagggtga gtccggtgag ttcagaatgt 2161 ctaactgttc agacatagaa agagtatgtt ttgagagcgt tggatactgg atgacttaca 2221 tattcaaggg aatggcgcat ggttcatgga acgatgcgac atattgtgac gggtcgtttg 2281 gtatggacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2341 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2401 tcgggtgggc ctttctgcgt ttatatacta gagttgacag ctagctcagt cctaggtact 2461 gtgctagcta ctagagtcac acaggaaagt actagagctc ctatagggcg aattgggccc 2521 tgcatgctcg agcggccgcc agtgtgatgg atataattcg cccttcattc caaaatgttg 2581 tttgacattg atacaggcta gagaggaggc ctaaatgtga gtaaagtttg tctttcacca 2641 tacatagttt tcactgagta aagcacgatg gaacaacttg aatgtgtctt cattgaaact 2701 gctgctctga ggctacagaa tgccagaacg gtagaaaaaa cgatttccgg tttcagggga 2761 cgatatctta gcaaggccag tgtttgaaga gggtgccaaa acagtgaaag ttcatttacc 2821 gggtaaagac gagtattggt tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2881 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2941 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttgac ggctagctca 3001 gtcctaggta cagtgctagc tactagagat taaagaggag aaatactaga gctttcatcc 3061 tttttccatt acttacctca atacatcatg tcaaatatac atatatatat actcatatgt 3121 aattcaaatt caaacaatca ataaccgaaa taaacctaat acgaacttac ttaaacaaaa 3181 cagcaacagg cactacactg aggactactc gacttttttt tttcgcggtt attgaccggt 3241 tgatccattt tttcatctat gtaatagttt aactcaatta accaattcaa ataccttaaa 3301 atatctaaat tcatgtattt gaccttttaa caacatttta cattattacc ctaaactttt 3361 aacttttatt caatttaatc tctaaatcta aaacttacac atttattaca atcgagccca 3421 atatatgcta gccaaattct ccttaacact cctatagctc tcaattaatg atattttcac 3481 aagaatttca ccttaatttt tttattttat cattttagtc cctaaacttt aaactaacta 3541 aatttacttt acaaaataat cctatttaac aacaaactta taaacctatc atttaacttc 3601 aaaaatatca aaaatcatca atggcaaatt tcaaaacctt tgacaatttt acaaaatagt 3661 ccctgagtta gctagattaa gctgcaacaa tttaaaaaac gggctttaga ttcactcaca 3721 tgcatagatc gattgtttgg agaagtttga agctttccta atcccctcct atggctttcg 3781 agcagtggaa agatgaagat ggcaaccatt tctctttctt tccaccttaa ttcattttta 3841 attactatta ttttcattat ttatttattt atcattttca acaaaattta ctagagccag 3901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4021 ttatatacta gagttgacgg ctagctcagt cctaggtaca gtgctagcta ctagagatta 4081 aagaggagaa atactagatg ttgtcggtag catcccgctc aggcccgttc gcgcccgtcc 4141 tgtcggccac gtcccgcggg gtggcgggcg cgctgcggcc cttggtgcag gccacggtgc 4201 ccgccacccc ggagcagcct gtgttggacc tgaagcggcc cttcctcagc cgggagtcgc 4261 tgagcggcca ggccgtgcgc cggcctttgg tcgcctccgt gggcctcaat gtccctgctt 4321 ctgtttgtta ttcccacaca gacatcaagg tgcctgactt ctctgaatac cgccgccttg 4381 aagttttaga tagtacgaag tcttcaagag aaagcagcga ggctaggaaa ggtttctcct 4441 atttggtaac tggagtaact actgtgggtg tcgcatatgc tgccaagaat gccgtcaccc 4501 agttcgtttc cagcatgagt gcttctgctg atgtgttggc cctggcgaaa atcgaaatca 4561 agttatccga tattccagaa ggcaagaaca tggctttcaa atggagaggc aaacccctgt 4621 ttgtgcgtca tagaacccag aaggaaattg agcaggaagt tgaattatca cagttgaggg 4681 acccacagca tgatcgagta aagaaacctg aatgggttat cctgataggt gtttgcactc 4741 atcttggctg tgtacccatt gcaaatgcag gagattttgg tggttattac tgcccttgcc 4801 atgggtcaca ctatgatgca tctggcagga tcagattggg tcctgctcct ctcaaccttg 4861 aagtccccac gtatgagttc accagtgacg atatggtgat tgttggttaa tactagagcc 4921 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4981 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 5041 gtttata //