##gff-version 3
##sequence-region BBa_K1196024 1 5047
BBa_K1196024	.	engineered_region	1	1361	.	+	0	ID=BBa_K1196018;Name=BBa_K1196018
BBa_K1196024	.	terminator	1233	1312	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1196018
BBa_K1196024	.	engineered_region	1233	1312	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1196024	.	stem_loop	1244	1287	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1196024	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1196018
BBa_K1196024	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1196024	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1196024	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1196024	.	terminator	1321	1361	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1196018
BBa_K1196024	.	stop_codon	1354	1354	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1196024	.	polyA_site	1348	1361	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1196024	.	stem_loop	1328	1347	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	1321	1361	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1196024	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1196018
BBa_K1196024	.	CDS	67	1224	.	+	0	ID=BBa_K1196009;Name=BBa_K1196009;Parent=BBa_K1196018
BBa_K1196024	.	engineered_region	1776	2425	.	+	0	ID=BBa_K1196020;Name=BBa_K1196020
BBa_K1196024	.	promoter	1776	1810	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1196020
BBa_K1196024	.	terminator	2297	2376	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1196020
BBa_K1196024	.	engineered_region	2297	2376	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1196024	.	stem_loop	2308	2351	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1196024	.	ribosome_entry_site	1819	1832	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K1196020
BBa_K1196024	.	conserved_region	1825	1828	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1196024	.	CDS	1839	2288	.	+	0	ID=BBa_K1196004;Name=BBa_K1196004;Parent=BBa_K1196020
BBa_K1196024	.	terminator	2385	2425	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1196020
BBa_K1196024	.	stop_codon	2418	2418	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1196024	.	polyA_site	2412	2425	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1196024	.	stem_loop	2392	2411	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	2385	2425	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	2434	2977	.	+	0	ID=BBa_K1196021;Name=BBa_K1196021
BBa_K1196024	.	ribosome_entry_site	2477	2489	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1196021
BBa_K1196024	.	ribosome_entry_site	2477	2489	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1196024	.	engineered_region	2477	2489	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1196024	.	ribosome_entry_site	2483	2486	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1196024	.	terminator	2937	2977	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1196021
BBa_K1196024	.	stop_codon	2970	2970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1196024	.	polyA_site	2964	2977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1196024	.	stem_loop	2944	2963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	2937	2977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1196024	.	promoter	2434	2468	.	+	0	ID=BBa_J23102;Name=BBa_J23102;Parent=BBa_K1196021
BBa_K1196024	.	terminator	2849	2928	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1196021
BBa_K1196024	.	engineered_region	2849	2928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1196024	.	stem_loop	2860	2903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1196024	.	CDS	2498	2840	.	+	0	ID=BBa_K1196014;Name=BBa_K1196014;Parent=BBa_K1196021
BBa_K1196024	.	engineered_region	4034	5047	.	+	0	ID=BBa_K1196023;Name=BBa_K1196023
BBa_K1196024	.	terminator	5007	5047	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1196023
BBa_K1196024	.	stop_codon	5040	5040	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1196024	.	polyA_site	5034	5047	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1196024	.	stem_loop	5014	5033	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	5007	5047	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1196024	.	CDS	4098	4910	.	+	0	ID=BBa_K1196015;Name=BBa_K1196015;Parent=BBa_K1196023
BBa_K1196024	.	ribosome_entry_site	4077	4091	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1196023
BBa_K1196024	.	ribosome_entry_site	4084	4088	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1196024	.	engineered_region	4077	4091	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1196024	.	ribosome_entry_site	4077	4091	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1196024	.	terminator	4919	4998	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1196023
BBa_K1196024	.	engineered_region	4919	4998	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1196024	.	stem_loop	4930	4973	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1196024	.	promoter	4034	4068	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1196023
BBa_K1196024	.	engineered_region	2986	4025	.	+	0	ID=BBa_K1196022;Name=BBa_K1196022
BBa_K1196024	.	CDS	3052	3888	.	+	0	ID=BBa_K1196013;Name=BBa_K1196013;Parent=BBa_K1196022
BBa_K1196024	.	promoter	2986	3020	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1196022
BBa_K1196024	.	terminator	3985	4025	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1196022
BBa_K1196024	.	stop_codon	4018	4018	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1196024	.	polyA_site	4012	4025	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1196024	.	stem_loop	3992	4011	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	3985	4025	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1196024	.	terminator	3897	3976	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1196022
BBa_K1196024	.	engineered_region	3897	3976	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1196024	.	stem_loop	3908	3951	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1196024	.	ribosome_entry_site	3029	3043	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1196022
BBa_K1196024	.	ribosome_entry_site	3036	3040	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1196024	.	engineered_region	3029	3043	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1196024	.	ribosome_entry_site	3029	3043	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1196024	.	engineered_region	1370	1767	.	+	0	ID=BBa_K1196019;Name=BBa_K1196019
BBa_K1196024	.	promoter	1370	1404	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K1196019
BBa_K1196024	.	ribosome_entry_site	1413	1427	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1196019
BBa_K1196024	.	ribosome_entry_site	1420	1424	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1196024	.	engineered_region	1413	1427	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1196024	.	ribosome_entry_site	1413	1427	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1196024	.	terminator	1639	1718	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1196019
BBa_K1196024	.	engineered_region	1639	1718	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1196024	.	stem_loop	1650	1693	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1196024	.	CDS	1436	1630	.	+	0	ID=BBa_K1196011;Name=BBa_K1196011;Parent=BBa_K1196019
BBa_K1196024	.	terminator	1727	1767	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1196019
BBa_K1196024	.	stop_codon	1760	1760	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1196024	.	polyA_site	1754	1767	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1196024	.	stem_loop	1734	1753	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1196024	.	engineered_region	1727	1767	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1196024
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagaggcaaggacctcta
ctatgatgacccgtcgagacactctgttgggcgccgccgcgaccgctctgctgctcagcgccgtgccgaacgtggcgtt
cgccgccgaggacgaaatctctaaactgccgcgccagaagattgagctggtggcgccgccgttcgtgcacgcgcatcag
caggccaccaaggacggcccgaaggtcgtcgagttcaagctggttgtccaggaaaaggaagtggtgatcgacgaggacg
gcaccaaattccaggcgatgacattcaacggctcgatgcccggaccgttgatggtggtgcacgagggcgactacgtcga
gctgacgctggtcaatcccgccaccaacaccatgccacacaatatcgacttccacgcctcgaccggcgcgctcggcggc
ggcgcgctgaccttgatcaatcccggcgaacaggtggtgctgcggttcaaggccgaccgcgccggcaccttcgtgtatc
actgcgcaccgggcggcaacatgatcccgtggcacgtcgtttccggcatgaacggcgccatgatggtgctgccgcgcga
cggcctgaaggatcggaacggcaagccgctgcactacgaccgcatctattacatcggcgagcaggacatgtacgtgccg
aaggacgagcacggcaaatacaaatccttcgcctcaccggccgagtcctatgtcgacacggtcgaggtgatgcgcaagc
tgatcccgacccacgtggtattcaacggcaaggtgggcgcgctgaccggcaaggacgcgatgaccgccaaggtcggcga
gaccgtgttgatcgtgcattcgcaggccaaccgcgacacccgcccgcacatcatcggcggccacggcgattatgtctgg
gagaccggcaagttcgccaatccgccggagaccaatctggagacctggttcatccgcggcggctcggccggagctgcgc
tgtacaccttcaagcagccgggcgtgtacgcctacgtcaaccacaacctgatcgaagcggtcgaactcggcgccaccgc
gcacttcaaggtcgacggcaagtggaacgacgacctgatgaagcaggtcagcgcccccggcccgatcccgggcggcccg
accaactaagactccgcgcttccgaggctcacgtttgtgtactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagagg
agaaatactagaggggtatcagtatgtagaggatgatggttcaaccgttacctcacagttggcagacgtaccgtattac
atccagattctggatgacaagggtatgtccgtacagacgggtttatcctgggcatacttaaggccgtatcatggtagga
ttggatgtcactatggatcatatcgtggaagggcatttaagaatttgcattactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactaga
gtcacacaggaaacctactagatgtcaaatgcagacagaggtgcgcctctctggaaggaaaagagagacacctgggtat
cagtatgtgacgattgccattcaccaaggtttgcaagagagaacttgcaggcgatggacgaagcttgtaaggatgcagg
tctgaagtatactgaaacgtttaaagtagcagagaatttgatgcttgatggtatgggcgagcctatgcctaaggatctt
catcctgactggagtggtcagcacatctggagtttgaagattggtgcttatcatgatggaccgaagtatggtggtaaga
agggtgagtccggtgagttcagaatgtctaactgttcagacatagaaagagtatgttttgagagcgttggatactggat
gacttacatattcaagggaatggcgcatggttcatggaacgatgcgacatattgtgacgggtcgtttggtatggactac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcag
tcctaggtactgtgctagctactagagtcacacaggaaagtactagagctcctatagggcgaattgggccctgcatgct
cgagcggccgccagtgtgatggatataattcgcccttcattccaaaatgttgtttgacattgatacaggctagagagga
ggcctaaatgtgagtaaagtttgtctttcaccatacatagttttcactgagtaaagcacgatggaacaacttgaatgtg
tcttcattgaaactgctgctctgaggctacagaatgccagaacggtagaaaaaacgatttccggtttcaggggacgata
tcttagcaaggccagtgtttgaagagggtgccaaaacagtgaaagttcatttaccgggtaaagacgagtattggttact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagt
cctaggtacagtgctagctactagagattaaagaggagaaatactagagctttcatcctttttccattacttacctcaa
tacatcatgtcaaatatacatatatatatactcatatgtaattcaaattcaaacaatcaataaccgaaataaacctaat
acgaacttacttaaacaaaacagcaacaggcactacactgaggactactcgacttttttttttcgcggttattgaccgg
ttgatccattttttcatctatgtaatagtttaactcaattaaccaattcaaataccttaaaatatctaaattcatgtat
ttgaccttttaacaacattttacattattaccctaaacttttaacttttattcaatttaatctctaaatctaaaactta
cacatttattacaatcgagcccaatatatgctagccaaattctccttaacactcctatagctctcaattaatgatattt
tcacaagaatttcaccttaatttttttattttatcattttagtccctaaactttaaactaactaaatttactttacaaa
ataatcctatttaacaacaaacttataaacctatcatttaacttcaaaaatatcaaaaatcatcaatggcaaatttcaa
aacctttgacaattttacaaaatagtccctgagttagctagattaagctgcaacaatttaaaaaacgggctttagattc
actcacatgcatagatcgattgtttggagaagtttgaagctttcctaatcccctcctatggctttcgagcagtggaaag
atgaagatggcaaccatttctctttctttccaccttaattcatttttaattactattattttcattatttatttattta
tcattttcaacaaaatttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgttgtcggt
agcatcccgctcaggcccgttcgcgcccgtcctgtcggccacgtcccgcggggtggcgggcgcgctgcggcccttggtg
caggccacggtgcccgccaccccggagcagcctgtgttggacctgaagcggcccttcctcagccgggagtcgctgagcg
gccaggccgtgcgccggcctttggtcgcctccgtgggcctcaatgtccctgcttctgtttgttattcccacacagacat
caaggtgcctgacttctctgaataccgccgccttgaagttttagatagtacgaagtcttcaagagaaagcagcgaggct
aggaaaggtttctcctatttggtaactggagtaactactgtgggtgtcgcatatgctgccaagaatgccgtcacccagt
tcgtttccagcatgagtgcttctgctgatgtgttggccctggcgaaaatcgaaatcaagttatccgatattccagaagg
caagaacatggctttcaaatggagaggcaaacccctgtttgtgcgtcatagaacccagaaggaaattgagcaggaagtt
gaattatcacagttgagggacccacagcatgatcgagtaaagaaacctgaatgggttatcctgataggtgtttgcactc
atcttggctgtgtacccattgcaaatgcaggagattttggtggttattactgcccttgccatgggtcacactatgatgc
atctggcaggatcagattgggtcctgctcctctcaaccttgaagtccccacgtatgagttcaccagtgacgatatggtg
attgttggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata