It contains the sequence of key enzymes in anammoxosome.
This part can synthesize hydrazine synthetase.
This part synthesize hydrazine oxidoreductase.
This part synthesize formate dehydrogenase.
NADH:ubiquinone reductase (H+-translocating) Device
This part synthesize ubiquinone reductase.
This part synthesize cytochrome C reductase.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
AUTHORS Yu,G.H., Chen,Y.H., Li,Y.J., Yang,Z.H. and Chen,Y.F. TITLE Cloning and sequence analysis of nitrite reductase from the nitrite-degrading strain 2-8 JOURNAL Unpublished REFERENCE 2 (bases 1 to 1158) AUTHORS Chen,Y.H., Yu,G.H., Li,Y.J., Chen,Y.F. and Yang,Z.H. TITLE Direct Submission JOURNAL Submitted (17-DEC-2009) Microbiology, Zhuhai Agricultural Sciences Research Center, Meixi Qianshan of Zhuhai City, Zhuhai, Guangdong 519070, China
nitrite reductase for Anammox Nitric oxide + H2O + Ferricytochrome c <=> Nitrite + Ferrocytochrome c + H+
Nitric oxide + H2O + Ferricytochrome c <=> Nitrite + Ferrocytochrome c + H+
RBS.3 (medium) -- derivative of BBa_0030
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
"Community structures of anammox bacteria and their similar distribution with nirS-encoding nitrite-reducing bacteria in surface sediment of the South China Sea." Li M., Hong Y., Cao H., Gu J. Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
Reaction(IUBMB) hydrazine + acceptor = N2 + reduced acceptor The enzyme is involved in the pathway of anaerobic ammonium oxidation in anammox bacteria. The conversion of hydroxylamine by the enzyme from Brocadia anammoxidans results in the formation of NO and N2O [1]. Hydroxylamine is not a substrate for the enzyme from planctomycete KSU-1
Reaction(IUBMB) hydrazine + acceptor = N2 + reduced acceptor
Reference: Han P., Klumper U., Wong A.C.F., Li M., Lin J.G., Quan Z.X., Ford T., Gu J.D. Submitted (26-MAR-2012) to the INSDC. School of Biological Sciences, The University of Hong Kong, Kadoorie Biological Sciences Building, Hong Kong, China The genomic sequence of this part comes from uncultured anaerobic ammonium-oxidizing bacterium.
Hydrazine synthetase, a unique phylomarker with which to study the presence and biodiversity of anammox bacteria.The tested hydrazine synthetase were able to retrieve hzsA gene sequences from anammox enrichment cultures, full-scale anammox wastewater treatment systems, and a variety of freshwater and marine environmental samples, covering all known anammox genera.
Authors DAVISON DC. Title Studies on plant formic dehydrogenase. Journal Biochem. J. 49 (1951) 520-6. Organism Phaseolus vulgaris
Oxidoreductases;Acting on the aldehyde or oxo group of donors;With NAD+ or NADP+ as acceptor. The enzyme from most aerobic organisms is devoid of redox-active centres but that from the proteobacterium Methylosinus trichosporium contains iron-sulfur centres, flavin and a molybdenum centre [3]. Together with EC 1.12.1.2 hydrogen dehydrogenase, forms a system previously known as formate hydrogenlyase.
NADH:ubiquinone reductase (H+-translocating)
Hatefi, Y., Ragan, C.I. and Galante, Y.M. The enzymes and the enzyme complexes of the mitochondrial oxidative phosphorylation system. In: Martonosi, A. (Ed.), The Enzymes of Biological Membranes, 2nd ed., vol. 4, Plenum Press, New York, 1985, p. 1-70.
Oxidoreductases; Acting on NADH or NADPH; With a quinone or similar compound as acceptor.
Metabolism; Energy metabolism; Oxidative phosphorylation [PATH:hsa00190] Metabolism; Energy metabolism; Nitrogen metabolism [PATH:hsa00910]
Activity is greater and more sensitive to inhibition by superoxide dismutase at pH 8.5 than pH 7.7. The epinephrine oxidation activity copurifies with NADPH-cytochrome c reductase.
Conversion of the iGEM parts registry to SBOL2.1