BBa_K606040
1
pHS
Promoter Hyperspank B.subtilis & E.coli
2011-09-17T11:00:00Z
2015-05-08T01:12:51Z
inspired from the part BBa_K143015
Released HQ 2013
Promoter hyper-spank is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG).
false
false
_778_
0
8931
9
In stock
false
DNA synthesis
false
Axel S??guret
annotation2138639
1
LacO
range2138639
1
72
92
annotation2138638
1
-10
range2138638
1
60
65
annotation2138637
1
-35
range2138637
1
37
41
annotation2138636
1
Lac0
range2138636
1
1
21
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K1197000
1
BBa_K1197000
Anti MazF - Antisense RNA to inhibit MazF activity in B.subtilis
2013-09-19T11:00:00Z
2015-06-08T11:15:20Z
It is synthesized as a reverse complementary sequence to MazF (K302033)
Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills Bacillus subtilis. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein.
false
false
_1511_
4206
12727
9
In stock
false
This part is designed as a complemantary sequence to the last 4 bases of the RBS sequence, 6 bases to the intermediate region and to the first 40 bases of MazF coding sequence, tottally 50 bases.
false
Alişan Kayab??len
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1197001
1
BBa_K1197001
Anti MazF construct (pHyperspank + Anti MazF + Double Terminator)
2013-09-19T11:00:00Z
2015-06-08T11:15:36Z
It is synthesized as a reverse complementary sequence to MazF (K302033)
Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills Bacillus subtilis. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein.
With pHyperspank promoter, the production of Anti MazF will be depend on IPTG presence in the medium. If there is IPTG in medium, it will inactivate LacI which inhibit pHyperspank promoter. Thus, Anti MazF will be produced. On the other hand, if there is no IPTG in medium, LacI will inhibit pHyperspank and Anti MazF mRNA cannot be produced. As a result MazF protein will be produced, and kills the bacteria.
false
false
_1511_
4206
12727
9
In stock
false
Anti MazF is designed as a complemantary sequence to the last 4 bases of the RBS sequence, 6 bases to the intermediate region and to the first 40 bases of MazF coding sequence, tottally 50 bases.
false
Alişan Kayab??len
component2355722
1
BBa_K1197000
component2355729
1
BBa_B0015
component2355721
1
BBa_K606040
annotation2355729
1
BBa_B0015
range2355729
1
159
287
annotation2355722
1
BBa_K1197000
range2355722
1
101
150
annotation2355721
1
BBa_K606040
range2355721
1
1
92
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1197000_sequence
1
aaatcagatcgcccatatcgggtacgtatcggcttaccatctagtattca
BBa_K1197001_sequence
1
aaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatttactagagaaatcagatcgcccatatcgggtacgtatcggcttaccatctagtattcatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K606040_sequence
1
aaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z