LOCUS       BBa_K1197005            1588 bp    DNA     linear       22-Dec-2024
DEFINITION  MazF +RBS (SpoVG) + LacI + DT
ACCESSION   BBa_K1197005
VERSION     BBa_K1197005.1  
FEATURES             Location/Qualifiers
     misc_feature    334..339
                     /label=double TAA added
     CDS             1..339
                     /label=BBa_K302033
     RBS             348..359
                     /label=SpoVG
     RBS             348..359
                     /label=BBa_K606061
     CDS             366..1451
                     /label=BBa_I732100
     stem_loop       1471..1514
                     /label=stem_loop
     misc_feature    1460..1539
                     /label=BBa_B0010
     terminator      1460..1539
                     /label=BBa_B0010
     stem_loop       1555..1574
                     /label=T7 TE
     misc_feature    1581..1581
                     /label=stop
     polyA_site      1575..1588
                     /label=polya
     misc_feature    1548..1588
                     /label=BBa_B0012
     terminator      1548..1588
                     /label=BBa_B0012
     terminator      1460..1588
                     /label=BBa_B0015
     misc_feature    348..1588
                     /label=BBa_K1197004
ORIGIN
        1 atggtaagcc gatacgtacc cgatatgggc gatctgattt gggttgattt tgacccgaca
       61 aaaggtagcg agcaagctgg acatcgtcca gctgttgtcc tgagtccttt catgtacaac
      121 aacaaaacag gtatgtgtct gtgtgttcct tgtacaacgc aatcaaaagg atatccgttc
      181 gaagttgttt tatccggtca ggaacgtgat ggcgtagcgt tagctgatca ggtaaaaagt
      241 atcgcctggc gggcaagagg agcaacgaag aaaggaacag ttgccccaga ggaattacaa
      301 ctcattaaag ccaaaattaa cgtactgatt gggtaataat actagagaaa ggtggtgaat
      361 actagatgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
      421 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
      481 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
      541 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
      601 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
      661 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
      721 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
      781 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
      841 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
      901 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
      961 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     1021 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     1081 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     1141 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     1201 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     1261 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     1321 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     1381 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1441 ggcagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1501 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1561 ccttcgggtg ggcctttctg cgtttata
//