LOCUS       BBa_I13501               843 bp    DNA     linear       29-Nov-2024
DEFINITION  Screening plasmid intermediate
ACCESSION   BBa_I13501
VERSION     BBa_I13501.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 ata
//
LOCUS       BBa_K1197008            1106 bp    DNA     linear       29-Nov-2024
DEFINITION  Pveg (K823003) + RBS (SpoVG) + RFP + DT
ACCESSION   BBa_K1197008
VERSION     BBa_K1197008.1  
FEATURES             Location/Qualifiers
     promoter        1..237
                     /label=promoter
     promoter        1..237
                     /label=BBa_K823003
     RBS             246..257
                     /label=rbs
     RBS             246..257
                     /label=BBa_K143021
     CDS             264..938
                     /label=mrfp1
     misc_feature    945..969
                     /label=Help:Barcodes
     CDS             264..944
                     /label=BBa_E1010
     stem_loop       989..1032
                     /label=stem_loop
     misc_feature    978..1057
                     /label=BBa_B0010
     terminator      978..1057
                     /label=BBa_B0010
     stem_loop       1073..1092
                     /label=T7 TE
     misc_feature    1099..1099
                     /label=stop
     polyA_site      1093..1106
                     /label=polya
     misc_feature    1066..1106
                     /label=BBa_B0012
     terminator      1066..1106
                     /label=BBa_B0012
     terminator      978..1106
                     /label=BBa_B0015
     CDS             264..1106
                     /label=BBa_K606012
     misc_feature    246..1106
                     /label=BBa_K606013
ORIGIN
        1 ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac ctgcatagga
       61 gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac ttgcggaaca
      121 taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt
      181 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagttac
      241 tagagaaagg tggtgaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      301 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      361 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      421 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      481 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      541 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      601 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      661 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      721 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
      781 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      841 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      901 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
      961 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1021 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1081 ttcgggtggg cctttctgcg tttata
//