BBa_K1197013 1 BBa_K1197013 CYP6G1 - Insecticide resistance gene 2013-10-01T11:00:00Z 2015-05-08T01:09:39Z It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis. It is an insecticide resistance gene which is found in Drosophila Melanogaster. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts. It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis. Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991) Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601. false false _1511_ 0 12727 9 Not in stock false Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991) Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601. false Alişan Kayab??len BBa_K1197013_sequence 1 atggctctgttattagcagtttttgtggtggtcgccgcactggtggcgctctacacttggttccagcgcaaccatagctactggcaacgcaagggcataccctatattccgcccacgccgatcattggcaacaccaaggtggtcttcaagatggagaactcctttgggatgcacctatcggagatatacaatgatccgcggctgaaggacgaggctgtggtgggcatctactccatgaacaagcccggcttgataatacgcgacatagagctgatcaaatccattctgatcaaggacttcaatcggttccacaaccgatacgcccgctgcgatccccatggcgatccattgggctataataacctgttcttcgtcagggatgcccattggaagggcattcgcaccaagctgactcccgttttcaccagcggcaaggtcaagcagatgtacacccttatgcaggagattggaaaggatctggagctggcacttcagaggcgtggagagaagaactctgggagtttcattacggagattaaggagatctgcgctcagttctccacggacagcatagccacgattgcatttggcattcgtgctaacagcctagagaatcccaacgcagagttccgtaactatggacgcaagatgttcaccttcaccgtagcgcgtgccaaggacttctttgtggccttcttcctgcccaagctggtgtcgctgatgcgcatccagttcttcacggcggacttttcccactttatgcgcagcaccattggtcacgttatggaggagcgagagcgatcgggcctgctccgcaatgatctcatagatgtcttggtgagtctgcgcaaagaggcggctgccgagccttcgaagcctcactatgccaagaaccaggacttcctggtggctcaggcgggcgtgttttttacggcgggtttcgagacctcctcctcgaccatgtcttttgccctgtacgagatggccaagcatccagagatgcagaaacgcctgcgcgacgagatcaacgaagctttggtggagggcggtgggtcattgagctacgagaaaatccagtccctggagtatctggccatggtggtggacgaggtgctgcgcatgtatccggtgctgccgttcctggaccgcgagtacgagagcgtggagggacagccagacttgagcctgaagccgttctacgactacactctcgagaacggaacccctgtgttcatacccatctatgcactgcatcatgatccaaagtactggaccaatcccagccaattcgatccggagcgtttctcacccgcgaaccgcaagaacatagtggccatggcatatcaacccttcggatctgggccgcacaactgcattggcagccggattggcctgctacagagcaaactgggactggtcagcctgctgaagaatcactcagtgcgcaactgcgaggccaccatgaaggacatgaaattcgatcccaagggtttcgtgctccaggcagatggtggcatacatttggagatagtcaacgatcgcctctacgatcagagcgctccatcgctccaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z