LOCUS BBa_K1197015 4087 bp DNA linear 25-Nov-2024 DEFINITION Catalytically active CYP6G1 coding device ACCESSION BBa_K1197015 VERSION BBa_K1197015.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_K143012 promoter 63..68 /label=Sigma A-35 promoter 86..91 /label=Sigma A -10 RBS 106..117 /label=rbs misc_feature 106..117 /label=BBa_K143021 misc_feature 1..117 /label=BBa_K143053 misc_feature 124..219 /label=SacB Signal tag promoter 1..219 /label=BBa_K541501 CDS 226..1803 /label=BBa_K1197013 RBS 1812..1823 /label=SpoVG RBS 1812..1823 /label=BBa_K606061 misc_feature 1830..1925 /label=SacB CDS 1932..3944 /label=fCPR misc_feature 3945..3947 /label=Stop misc_feature 3948..3950 /label=Stop CDS 1830..3950 /label=BBa_K1197016 stem_loop 3970..4013 /label=stem_loop misc_feature 3959..4038 /label=BBa_B0010 terminator 3959..4038 /label=BBa_B0010 stem_loop 4054..4073 /label=T7 TE misc_feature 4080..4080 /label=stop polyA_site 4074..4087 /label=polya misc_feature 4047..4087 /label=BBa_B0012 terminator 4047..4087 /label=BBa_B0012 terminator 3959..4087 /label=BBa_B0015 ORIGIN 1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt 61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac 121 tagatgaaca tcaaaaaatt tgcaaaacag gcgacagttc tgacatttac aacagcactg 181 cttgcaggcg gcgcaacaca agcatttgca aaagaaacat actagatggc tctgttatta 241 gcagtttttg tggtggtcgc cgcactggtg gcgctctaca cttggttcca gcgcaaccat 301 agctactggc aacgcaaggg cataccctat attccgccca cgccgatcat tggcaacacc 361 aaggtggtct tcaagatgga gaactccttt gggatgcacc tatcggagat atacaatgat 421 ccgcggctga aggacgaggc tgtggtgggc atctactcca tgaacaagcc cggcttgata 481 atacgcgaca tagagctgat caaatccatt ctgatcaagg acttcaatcg gttccacaac 541 cgatacgccc gctgcgatcc ccatggcgat ccattgggct ataataacct gttcttcgtc 601 agggatgccc attggaaggg cattcgcacc aagctgactc ccgttttcac cagcggcaag 661 gtcaagcaga tgtacaccct tatgcaggag attggaaagg atctggagct ggcacttcag 721 aggcgtggag agaagaactc tgggagtttc attacggaga ttaaggagat ctgcgctcag 781 ttctccacgg acagcatagc cacgattgca tttggcattc gtgctaacag cctagagaat 841 cccaacgcag agttccgtaa ctatggacgc aagatgttca ccttcaccgt agcgcgtgcc 901 aaggacttct ttgtggcctt cttcctgccc aagctggtgt cgctgatgcg catccagttc 961 ttcacggcgg acttttccca ctttatgcgc agcaccattg gtcacgttat ggaggagcga 1021 gagcgatcgg gcctgctccg caatgatctc atagatgtct tggtgagtct gcgcaaagag 1081 gcggctgccg agccttcgaa gcctcactat gccaagaacc aggacttcct ggtggctcag 1141 gcgggcgtgt tttttacggc gggtttcgag acctcctcct cgaccatgtc ttttgccctg 1201 tacgagatgg ccaagcatcc agagatgcag aaacgcctgc gcgacgagat caacgaagct 1261 ttggtggagg gcggtgggtc attgagctac gagaaaatcc agtccctgga gtatctggcc 1321 atggtggtgg acgaggtgct gcgcatgtat ccggtgctgc cgttcctgga ccgcgagtac 1381 gagagcgtgg agggacagcc agacttgagc ctgaagccgt tctacgacta cactctcgag 1441 aacggaaccc ctgtgttcat acccatctat gcactgcatc atgatccaaa gtactggacc 1501 aatcccagcc aattcgatcc ggagcgtttc tcacccgcga accgcaagaa catagtggcc 1561 atggcatatc aacccttcgg atctgggccg cacaactgca ttggcagccg gattggcctg 1621 ctacagagca aactgggact ggtcagcctg ctgaagaatc actcagtgcg caactgcgag 1681 gccaccatga aggacatgaa attcgatccc aagggtttcg tgctccaggc agatggtggc 1741 atacatttgg agatagtcaa cgatcgcctc tacgatcaga gcgctccatc gctccaataa 1801 taatactaga gaaaggtggt gaatactaga tgaacatcaa aaaatttgca aaacaggcga 1861 cagttctgac atttacaaca gcactgcttg caggcggcgc aacacaagca tttgcaaaag 1921 aaacatacta gatggctgcg gaacacgtcg aagaagtggt ttcggaagaa ccatttttgg 1981 gcacactgga tattgcatta ttagtagtcc tcttagtcgg tgcaacatgg tactttatga 2041 gaagtcgtaa gaaggaagaa gctccaattc gttcatattc tatacaaccc actacagtca 2101 gcaccgtttc aacgacagag aactcgttca taaaaaaatt gaaagcttca ggtcgtagtt 2161 tagttgtgtt ttatggttcc cagaccggaa cagccgaaga atttgccgga cgattggcca 2221 aggaaggttt acgctatcgc atgaagggaa tggtggcgga ccccgaagaa tgtgacatgg 2281 aagaattgtt acaaatgaag gatattccta actcattagc cgttttctgt ttggctacat 2341 atggtgaagg agatcccact gataacgcta tggaatttta tgaatggatt acaaatggcg 2401 aggttgattt gacgggttta aactacgccg tatttggctt aggcaacaag acttatgaac 2461 attacaacaa agttgcaatt tatgtggaca agaggttgga ggaactcggt gctacgagag 2521 tgttcgagtt aggtcttgga gacgatgatg caaatatcga agatgacttc attacgtgga 2581 aagacagatt ttggccatct gtttgtgatt tcttcggaat cgaaggaagt ggtgaggagg 2641 tattgatgcg tcaattccga cttttggaac agccggatgt tcaacccgat cgtatttaca 2701 ccggcgaaat agctcggttg cactcaatgc aaaatcaaag acctccattt gatgctaaaa 2761 atccattttt ggcttcagtc atagttaatc gagagctgca taaaggcggc ggacggtcat 2821 gtatgcatat tgagttggat attgatggat caaaaatgcg gtatgatgca ggcgaccata 2881 tagcaatgta tccgattaat gataaaatcc tggtagaaaa attgggtaaa ctatgtgatg 2941 caaatctgga tacagtcttc tctttgatca atacggatac ggatagcagt aaaaaacatc 3001 cattcccctg tcccaccaca taccgtaccg ccctgacaca ttacttggag attactgcaa 3061 taccgagaac acatattctg aaggagttag cagagtattg ttccgatgaa aaggataagg 3121 aatttttacg caacatggcg tctattactc cggaaggcaa agagaagtat cagaattgga 3181 tccagaactc ttcaagaaat attgtgcata ttttggagga tatcaaatct tgtagaccac 3241 caattgatca tatttgtgaa ctgttgccaa gacttcaacc gcgttattac tcgatttcat 3301 cttcttctaa gctctatcca actaatgttc atattacagc agttctggtt caatatgaaa 3361 cacctactgg ccgtgtaaac aaaggtgttg ctacgtcata catgaaggaa aaaaatcctt 3421 cggttggaga ggttaaagtg cctgtattta ttcgcaaatc tcaatttagg ttgccaacta 3481 aatccgagat tccaatcata atggtgggcc caggaacggg tctagcacct ttcagaggat 3541 tcatacagga aagacaattt ttacgagatg gaggaaaagt tgtcggcgat accatattgt 3601 atttcgggtg tagaaagaag gacgaagact tcatttatag agaagaacta gagcaatatg 3661 tacaaaatgg aactttaacc ttgaaaacgg cattttcaag agaccaacaa gaaaaaattt 3721 atgttactca tctaatcgaa caagatgctg atttaatttg gaaagtaatt ggcgaacaaa 3781 agggacactt ttatatttgc ggggatgcta aaaatatggc tgtggatgtt agaaatattt 3841 tagttaaaat tttgtcaacc aaaggcaata tgaacgaatc agatgctgtg caatatataa 3901 aaaagatgga ggcacaaaag cgttattccg cggacgtttg gagctaataa tactagagcc 3961 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 4021 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 4081 gtttata //