BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1197015 1 BBa_K1197015 Catalytically active CYP6G1 coding device 2013-10-01T11:00:00Z 2015-05-08T01:09:39Z CYP6G1 comes from Drosophila melanogaster genome and fCPR comes from Musca domestica. This device was constructed to produce catalytically active CYP6G1 in B. subtilis and secrete it outside. Secretion is needed since CYP6G1 enzyme should degrade imidacloprid in bee midgut. To provide constitutively production and secretion of this enzyme, we used Pveg constitutive promoter, SpoVG RBS with SacB signal peptide (K541501) which is used by Fatih_Turkey Team in 2011. Also fCPR production is necessary to make CYP6G1 enzyme catalytically active. Thus, SpoVG RBS and SacB signal peptide was used before fCPR too. false false _1511_ 0 12727 9 Not in stock false Promoter and RBS are specific for expression and signal peptide is specific for secretion in B. subtilis. false Ali&#351;an Kayab??len component2367421 1 BBa_K541501 component2367429 1 BBa_K1197016 component2367424 1 BBa_K606061 component2367422 1 BBa_K1197013 component2367436 1 BBa_B0015 annotation2367421 1 BBa_K541501 range2367421 1 1 219 annotation2367422 1 BBa_K1197013 range2367422 1 226 1803 annotation2367429 1 BBa_K1197016 range2367429 1 1830 3950 annotation2367424 1 BBa_K606061 range2367424 1 1812 1823 annotation2367436 1 BBa_B0015 range2367436 1 3959 4087 BBa_K1197016 1 BBa_K1197016 fCPR with SacB 2013-10-01T11:00:00Z 2015-05-08T01:09:39Z The source of fCPR is Musca domestica genome, and SacB comes from B. subtilis. fCPR is a cytochrome P450 reductase which makes CYP6G1 enzyme catalytically active. And SacB is a signal peptide in B. subtilis which directs produced proteins outside of the cell. false false _1511_ 0 12727 9 Not in stock false SacB sequence was added to upstream of fCPR sequence to create a fusion protein which secrete outside of the cell. false Ali&#351;an Kayab??len annotation2367395 1 fCPR range2367395 1 103 2115 annotation2367396 1 Stop range2367396 1 2116 2118 annotation2367397 1 Stop range2367397 1 2119 2121 annotation2367394 1 SacB range2367394 1 1 96 BBa_K606061 1 BBa_K606061 SpoVG Ribosome Binding Site (RBS) for B. subtilis 2011-09-18T11:00:00Z 2015-05-08T01:12:51Z SYnthesized RBS because part BBa_K143021 was wrong. Released HQ 2013 SpoVG Ribosome Binding Site (RBS) for B. subtilis false false _778_ 0 8931 9 In stock false RBS false Axel S??guret annotation2160544 1 SpoVG range2160544 1 1 12 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K541501 1 BBa_K541501 Promoter Pveg, RBS spoVG and SacB signal peptide for B. subtilis 2011-07-18T11:00:00Z 2015-05-08T01:12:38Z All three basic components come from B.subtilis genome Released HQ 2013 Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis. SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by B. subtilis. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium. false false _708_ 0 4260 9 In stock false no any design consideration false Cihan TA&#350;TAN annotation2123457 1 rbs range2123457 1 106 117 annotation2123456 1 Sigma A -10 range2123456 1 86 91 annotation2123455 1 Sigma A-35 range2123455 1 63 68 annotation2123454 1 BBa_K143012 range2123454 1 1 97 annotation2123459 1 BBa_K143053 range2123459 1 1 117 annotation2123460 1 SacB Signal tag range2123460 1 124 219 annotation2123458 1 BBa_K143021 range2123458 1 106 117 BBa_K1197013 1 BBa_K1197013 CYP6G1 - Insecticide resistance gene 2013-10-01T11:00:00Z 2015-05-08T01:09:39Z It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis. It is an insecticide resistance gene which is found in Drosophila Melanogaster. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts. It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis. Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991) Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601. false false _1511_ 0 12727 9 Not in stock false Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991) Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601. false Ali&#351;an Kayab??len BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1197013_sequence 1 atggctctgttattagcagtttttgtggtggtcgccgcactggtggcgctctacacttggttccagcgcaaccatagctactggcaacgcaagggcataccctatattccgcccacgccgatcattggcaacaccaaggtggtcttcaagatggagaactcctttgggatgcacctatcggagatatacaatgatccgcggctgaaggacgaggctgtggtgggcatctactccatgaacaagcccggcttgataatacgcgacatagagctgatcaaatccattctgatcaaggacttcaatcggttccacaaccgatacgcccgctgcgatccccatggcgatccattgggctataataacctgttcttcgtcagggatgcccattggaagggcattcgcaccaagctgactcccgttttcaccagcggcaaggtcaagcagatgtacacccttatgcaggagattggaaaggatctggagctggcacttcagaggcgtggagagaagaactctgggagtttcattacggagattaaggagatctgcgctcagttctccacggacagcatagccacgattgcatttggcattcgtgctaacagcctagagaatcccaacgcagagttccgtaactatggacgcaagatgttcaccttcaccgtagcgcgtgccaaggacttctttgtggccttcttcctgcccaagctggtgtcgctgatgcgcatccagttcttcacggcggacttttcccactttatgcgcagcaccattggtcacgttatggaggagcgagagcgatcgggcctgctccgcaatgatctcatagatgtcttggtgagtctgcgcaaagaggcggctgccgagccttcgaagcctcactatgccaagaaccaggacttcctggtggctcaggcgggcgtgttttttacggcgggtttcgagacctcctcctcgaccatgtcttttgccctgtacgagatggccaagcatccagagatgcagaaacgcctgcgcgacgagatcaacgaagctttggtggagggcggtgggtcattgagctacgagaaaatccagtccctggagtatctggccatggtggtggacgaggtgctgcgcatgtatccggtgctgccgttcctggaccgcgagtacgagagcgtggagggacagccagacttgagcctgaagccgttctacgactacactctcgagaacggaacccctgtgttcatacccatctatgcactgcatcatgatccaaagtactggaccaatcccagccaattcgatccggagcgtttctcacccgcgaaccgcaagaacatagtggccatggcatatcaacccttcggatctgggccgcacaactgcattggcagccggattggcctgctacagagcaaactgggactggtcagcctgctgaagaatcactcagtgcgcaactgcgaggccaccatgaaggacatgaaattcgatcccaagggtttcgtgctccaggcagatggtggcatacatttggagatagtcaacgatcgcctctacgatcagagcgctccatcgctccaataataa BBa_K541501_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaaca BBa_K1197016_sequence 1 atgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggctgcggaacacgtcgaagaagtggtttcggaagaaccatttttgggcacactggatattgcattattagtagtcctcttagtcggtgcaacatggtactttatgagaagtcgtaagaaggaagaagctccaattcgttcatattctatacaacccactacagtcagcaccgtttcaacgacagagaactcgttcataaaaaaattgaaagcttcaggtcgtagtttagttgtgttttatggttcccagaccggaacagccgaagaatttgccggacgattggccaaggaaggtttacgctatcgcatgaagggaatggtggcggaccccgaagaatgtgacatggaagaattgttacaaatgaaggatattcctaactcattagccgttttctgtttggctacatatggtgaaggagatcccactgataacgctatggaattttatgaatggattacaaatggcgaggttgatttgacgggtttaaactacgccgtatttggcttaggcaacaagacttatgaacattacaacaaagttgcaatttatgtggacaagaggttggaggaactcggtgctacgagagtgttcgagttaggtcttggagacgatgatgcaaatatcgaagatgacttcattacgtggaaagacagattttggccatctgtttgtgatttcttcggaatcgaaggaagtggtgaggaggtattgatgcgtcaattccgacttttggaacagccggatgttcaacccgatcgtatttacaccggcgaaatagctcggttgcactcaatgcaaaatcaaagacctccatttgatgctaaaaatccatttttggcttcagtcatagttaatcgagagctgcataaaggcggcggacggtcatgtatgcatattgagttggatattgatggatcaaaaatgcggtatgatgcaggcgaccatatagcaatgtatccgattaatgataaaatcctggtagaaaaattgggtaaactatgtgatgcaaatctggatacagtcttctctttgatcaatacggatacggatagcagtaaaaaacatccattcccctgtcccaccacataccgtaccgccctgacacattacttggagattactgcaataccgagaacacatattctgaaggagttagcagagtattgttccgatgaaaaggataaggaatttttacgcaacatggcgtctattactccggaaggcaaagagaagtatcagaattggatccagaactcttcaagaaatattgtgcatattttggaggatatcaaatcttgtagaccaccaattgatcatatttgtgaactgttgccaagacttcaaccgcgttattactcgatttcatcttcttctaagctctatccaactaatgttcatattacagcagttctggttcaatatgaaacacctactggccgtgtaaacaaaggtgttgctacgtcatacatgaaggaaaaaaatccttcggttggagaggttaaagtgcctgtatttattcgcaaatctcaatttaggttgccaactaaatccgagattccaatcataatggtgggcccaggaacgggtctagcacctttcagaggattcatacaggaaagacaatttttacgagatggaggaaaagttgtcggcgataccatattgtatttcgggtgtagaaagaaggacgaagacttcatttatagagaagaactagagcaatatgtacaaaatggaactttaaccttgaaaacggcattttcaagagaccaacaagaaaaaatttatgttactcatctaatcgaacaagatgctgatttaatttggaaagtaattggcgaacaaaagggacacttttatatttgcggggatgctaaaaatatggctgtggatgttagaaatattttagttaaaattttgtcaaccaaaggcaatatgaacgaatcagatgctgtgcaatatataaaaaagatggaggcacaaaagcgttattccgcggacgtttggagctaataa BBa_K1197015_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggctctgttattagcagtttttgtggtggtcgccgcactggtggcgctctacacttggttccagcgcaaccatagctactggcaacgcaagggcataccctatattccgcccacgccgatcattggcaacaccaaggtggtcttcaagatggagaactcctttgggatgcacctatcggagatatacaatgatccgcggctgaaggacgaggctgtggtgggcatctactccatgaacaagcccggcttgataatacgcgacatagagctgatcaaatccattctgatcaaggacttcaatcggttccacaaccgatacgcccgctgcgatccccatggcgatccattgggctataataacctgttcttcgtcagggatgcccattggaagggcattcgcaccaagctgactcccgttttcaccagcggcaaggtcaagcagatgtacacccttatgcaggagattggaaaggatctggagctggcacttcagaggcgtggagagaagaactctgggagtttcattacggagattaaggagatctgcgctcagttctccacggacagcatagccacgattgcatttggcattcgtgctaacagcctagagaatcccaacgcagagttccgtaactatggacgcaagatgttcaccttcaccgtagcgcgtgccaaggacttctttgtggccttcttcctgcccaagctggtgtcgctgatgcgcatccagttcttcacggcggacttttcccactttatgcgcagcaccattggtcacgttatggaggagcgagagcgatcgggcctgctccgcaatgatctcatagatgtcttggtgagtctgcgcaaagaggcggctgccgagccttcgaagcctcactatgccaagaaccaggacttcctggtggctcaggcgggcgtgttttttacggcgggtttcgagacctcctcctcgaccatgtcttttgccctgtacgagatggccaagcatccagagatgcagaaacgcctgcgcgacgagatcaacgaagctttggtggagggcggtgggtcattgagctacgagaaaatccagtccctggagtatctggccatggtggtggacgaggtgctgcgcatgtatccggtgctgccgttcctggaccgcgagtacgagagcgtggagggacagccagacttgagcctgaagccgttctacgactacactctcgagaacggaacccctgtgttcatacccatctatgcactgcatcatgatccaaagtactggaccaatcccagccaattcgatccggagcgtttctcacccgcgaaccgcaagaacatagtggccatggcatatcaacccttcggatctgggccgcacaactgcattggcagccggattggcctgctacagagcaaactgggactggtcagcctgctgaagaatcactcagtgcgcaactgcgaggccaccatgaaggacatgaaattcgatcccaagggtttcgtgctccaggcagatggtggcatacatttggagatagtcaacgatcgcctctacgatcagagcgctccatcgctccaataataatactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggctgcggaacacgtcgaagaagtggtttcggaagaaccatttttgggcacactggatattgcattattagtagtcctcttagtcggtgcaacatggtactttatgagaagtcgtaagaaggaagaagctccaattcgttcatattctatacaacccactacagtcagcaccgtttcaacgacagagaactcgttcataaaaaaattgaaagcttcaggtcgtagtttagttgtgttttatggttcccagaccggaacagccgaagaatttgccggacgattggccaaggaaggtttacgctatcgcatgaagggaatggtggcggaccccgaagaatgtgacatggaagaattgttacaaatgaaggatattcctaactcattagccgttttctgtttggctacatatggtgaaggagatcccactgataacgctatggaattttatgaatggattacaaatggcgaggttgatttgacgggtttaaactacgccgtatttggcttaggcaacaagacttatgaacattacaacaaagttgcaatttatgtggacaagaggttggaggaactcggtgctacgagagtgttcgagttaggtcttggagacgatgatgcaaatatcgaagatgacttcattacgtggaaagacagattttggccatctgtttgtgatttcttcggaatcgaaggaagtggtgaggaggtattgatgcgtcaattccgacttttggaacagccggatgttcaacccgatcgtatttacaccggcgaaatagctcggttgcactcaatgcaaaatcaaagacctccatttgatgctaaaaatccatttttggcttcagtcatagttaatcgagagctgcataaaggcggcggacggtcatgtatgcatattgagttggatattgatggatcaaaaatgcggtatgatgcaggcgaccatatagcaatgtatccgattaatgataaaatcctggtagaaaaattgggtaaactatgtgatgcaaatctggatacagtcttctctttgatcaatacggatacggatagcagtaaaaaacatccattcccctgtcccaccacataccgtaccgccctgacacattacttggagattactgcaataccgagaacacatattctgaaggagttagcagagtattgttccgatgaaaaggataaggaatttttacgcaacatggcgtctattactccggaaggcaaagagaagtatcagaattggatccagaactcttcaagaaatattgtgcatattttggaggatatcaaatcttgtagaccaccaattgatcatatttgtgaactgttgccaagacttcaaccgcgttattactcgatttcatcttcttctaagctctatccaactaatgttcatattacagcagttctggttcaatatgaaacacctactggccgtgtaaacaaaggtgttgctacgtcatacatgaaggaaaaaaatccttcggttggagaggttaaagtgcctgtatttattcgcaaatctcaatttaggttgccaactaaatccgagattccaatcataatggtgggcccaggaacgggtctagcacctttcagaggattcatacaggaaagacaatttttacgagatggaggaaaagttgtcggcgataccatattgtatttcgggtgtagaaagaaggacgaagacttcatttatagagaagaactagagcaatatgtacaaaatggaactttaaccttgaaaacggcattttcaagagaccaacaagaaaaaatttatgttactcatctaatcgaacaagatgctgatttaatttggaaagtaattggcgaacaaaagggacacttttatatttgcggggatgctaaaaatatggctgtggatgttagaaatattttagttaaaattttgtcaaccaaaggcaatatgaacgaatcagatgctgtgcaatatataaaaaagatggaggcacaaaagcgttattccgcggacgtttggagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K606061_sequence 1 aaaggtggtgaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z