BBa_K1197015
type: ComponentDefinition
persistent identity: BBa K1197015
display id: BBa_K1197015
version: 1
was generated by: iGEM to SBOL conversion
description: Catalytically active CYP6G1 coding device
created: 2013-10-01T11:00:00Z
modified: 2015-05-08T01:09:39Z
mutable provenance: CYP6G1 comes from Drosophila melanogaster genome and fCPR comes from Musca domestica.
owned by: james
owned by: myers
mutable description: This device was constructed to produce catalytically active CYP6G1 in B. subtilis and secrete it outside. Secretion is needed since CYP6G1 enzyme should degrade imidacloprid in bee midgut. To provide constitutively production and secretion of this enzyme, we used Pveg constitutive promoter, SpoVG RBS with SacB signal peptide (K541501) which is used by Fatih_Turkey Team in 2011. Also fCPR production is necessary to make CYP6G1 enzyme catalytically active. Thus, SpoVG RBS and SacB signal peptide was used before fCPR too.
discontinued: false
dominant: false
group u list: _1511_
m user id: 0
owner id: 12727
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Promoter and RBS are specific for expression and signal peptide is specific for secretion in B. subtilis.
star: false
creator: Alişan Kayab??len
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K1197016
component: BBa_K541501
component: BBa_K606061
component: BBa_K1197013
sequence annotation: BBa_K541501
sequence annotation: BBa_K1197013
sequence annotation: BBa_K606061
sequence annotation: BBa_K1197016
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1197015_sequence/1

BBa_B0015
type: Component
persistent identity: component2367436
display id: component2367436
version: 1
top level: BBa_K1197015
definition: BBa_B0015
access: public

BBa_K1197016
type: Component
persistent identity: component2367429
display id: component2367429
version: 1
top level: BBa_K1197015
definition: BBa_K1197016
access: public

BBa_K541501
type: Component
persistent identity: component2367421
display id: component2367421
version: 1
top level: BBa_K1197015
definition: BBa_K541501
access: public

BBa_K606061
type: Component
persistent identity: component2367424
display id: component2367424
version: 1
top level: BBa_K1197015
definition: BBa_K606061
access: public

BBa_K1197013
type: Component
persistent identity: component2367422
display id: component2367422
version: 1
top level: BBa_K1197015
definition: BBa_K1197013
access: public

BBa_K541501
type: SequenceAnnotation
persistent identity: annotation2367421
display id: annotation2367421
version: 1
top level: BBa_K1197015
location: https://synbiohub.org/public/igem/BBa_K1197015/annotation2367421/range2367421/1
component: BBa_K541501

https://synbiohub.org/public/igem/BBa_K1197015/annotation2367421/range2367421/1
type: Range
persistent identity: range2367421
display id: range2367421
version: 1
top level: BBa_K1197015
direction: direction/reverse
start: 1
end: 219
orientation: inline

BBa_K1197013
type: SequenceAnnotation
persistent identity: annotation2367422
display id: annotation2367422
version: 1
top level: BBa_K1197015
location: https://synbiohub.org/public/igem/BBa_K1197015/annotation2367422/range2367422/1
component: BBa_K1197013

https://synbiohub.org/public/igem/BBa_K1197015/annotation2367422/range2367422/1
type: Range
persistent identity: range2367422
display id: range2367422
version: 1
top level: BBa_K1197015
direction: direction/reverse
start: 226
end: 1803
orientation: inline

BBa_K606061
type: SequenceAnnotation
persistent identity: annotation2367424
display id: annotation2367424
version: 1
top level: BBa_K1197015
location: https://synbiohub.org/public/igem/BBa_K1197015/annotation2367424/range2367424/1
component: BBa_K606061

https://synbiohub.org/public/igem/BBa_K1197015/annotation2367424/range2367424/1
type: Range
persistent identity: range2367424
display id: range2367424
version: 1
top level: BBa_K1197015
direction: direction/reverse
start: 1812
end: 1823
orientation: inline

BBa_K1197016
type: SequenceAnnotation
persistent identity: annotation2367429
display id: annotation2367429
version: 1
top level: BBa_K1197015
location: https://synbiohub.org/public/igem/BBa_K1197015/annotation2367429/range2367429/1
component: BBa_K1197016

https://synbiohub.org/public/igem/BBa_K1197015/annotation2367429/range2367429/1
type: Range
persistent identity: range2367429
display id: range2367429
version: 1
top level: BBa_K1197015
direction: direction/reverse
start: 1830
end: 3950
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2367436
display id: annotation2367436
version: 1
top level: BBa_K1197015
location: https://synbiohub.org/public/igem/BBa_K1197015/annotation2367436/range2367436/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1197015/annotation2367436/range2367436/1
type: Range
persistent identity: range2367436
display id: range2367436
version: 1
top level: BBa_K1197015
direction: direction/reverse
start: 3959
end: 4087
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1197013
type: ComponentDefinition
persistent identity: BBa K1197013
display id: BBa_K1197013
version: 1
was generated by: iGEM to SBOL conversion
description: CYP6G1 - Insecticide resistance gene
created: 2013-10-01T11:00:00Z
modified: 2015-05-08T01:09:39Z
mutable provenance: It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis.
owned by: james
owned by: myers
mutable description: It is an insecticide resistance gene which is found in Drosophila Melanogaster. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts. It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis. Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991) Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601.
discontinued: false
dominant: false
group u list: _1511_
m user id: 0
owner id: 12727
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991) Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601.
star: false
creator: Alişan Kayab??len
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1197013_sequence/1

BBa_K1197016
type: ComponentDefinition
persistent identity: BBa K1197016
display id: BBa_K1197016
version: 1
was generated by: iGEM to SBOL conversion
description: fCPR with SacB
created: 2013-10-01T11:00:00Z
modified: 2015-05-08T01:09:39Z
mutable provenance: The source of fCPR is Musca domestica genome, and SacB comes from B. subtilis.
owned by: james
owned by: myers
mutable description: fCPR is a cytochrome P450 reductase which makes CYP6G1 enzyme catalytically active. And SacB is a signal peptide in B. subtilis which directs produced proteins outside of the cell.
discontinued: false
dominant: false
group u list: _1511_
m user id: 0
owner id: 12727
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: SacB sequence was added to upstream of fCPR sequence to create a fusion protein which secrete outside of the cell.
star: false
creator: Alişan Kayab??len
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: SacB
sequence annotation: fCPR
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1197016_sequence/1

SacB
type: SequenceAnnotation
persistent identity: annotation2367394
display id: annotation2367394
version: 1
top level: BBa_K1197016
location: https://synbiohub.org/public/igem/BBa_K1197016/annotation2367394/range2367394/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1197016/annotation2367394/range2367394/1
type: Range
persistent identity: range2367394
display id: range2367394
version: 1
top level: BBa_K1197016
direction: direction/forward
start: 1
end: 96
orientation: inline

fCPR
type: SequenceAnnotation
persistent identity: annotation2367395
display id: annotation2367395
version: 1
top level: BBa_K1197016
location: https://synbiohub.org/public/igem/BBa_K1197016/annotation2367395/range2367395/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1197016/annotation2367395/range2367395/1
type: Range
persistent identity: range2367395
display id: range2367395
version: 1
top level: BBa_K1197016
direction: direction/forward
start: 103
end: 2115
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2367396
display id: annotation2367396
version: 1
top level: BBa_K1197016
location: https://synbiohub.org/public/igem/BBa_K1197016/annotation2367396/range2367396/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1197016/annotation2367396/range2367396/1
type: Range
persistent identity: range2367396
display id: range2367396
version: 1
top level: BBa_K1197016
direction: direction/forward
start: 2116
end: 2118
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2367397
display id: annotation2367397
version: 1
top level: BBa_K1197016
location: https://synbiohub.org/public/igem/BBa_K1197016/annotation2367397/range2367397/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1197016/annotation2367397/range2367397/1
type: Range
persistent identity: range2367397
display id: range2367397
version: 1
top level: BBa_K1197016
direction: direction/forward
start: 2119
end: 2121
orientation: inline

BBa_K541501
type: ComponentDefinition
persistent identity: BBa K541501
display id: BBa_K541501
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg, RBS spoVG and SacB signal peptide for B. subtilis
created: 2011-07-18T11:00:00Z
modified: 2015-05-08T01:12:38Z
mutable provenance: All three basic components come from B.subtilis genome
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis. SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by B. subtilis. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium.
discontinued: false
dominant: false
experience: experience/None
group u list: _708_
m user id: 0
owner id: 4260
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no any design consideration
star: false
creator: Cihan TAŞTAN
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_K143053
sequence annotation: BBa_K143012
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence annotation: rbs
sequence annotation: BBa_K143021
sequence annotation: SacB Signal tag
sequence: https://synbiohub.org/public/igem/BBa_K541501_sequence/1

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2123459
display id: annotation2123459
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123459/range2123459/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K541501/annotation2123459/range2123459/1
type: Range
persistent identity: range2123459
display id: range2123459
version: 1
top level: BBa_K541501
direction: direction/reverse
start: 1
end: 117
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2123454
display id: annotation2123454
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123454/range2123454/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K541501/annotation2123454/range2123454/1
type: Range
persistent identity: range2123454
display id: range2123454
version: 1
top level: BBa_K541501
direction: direction/reverse
start: 1
end: 97
orientation: inline

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation2123455
display id: annotation2123455
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123455/range2123455/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K541501/annotation2123455/range2123455/1
type: Range
persistent identity: range2123455
display id: range2123455
version: 1
top level: BBa_K541501
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation2123456
display id: annotation2123456
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123456/range2123456/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K541501/annotation2123456/range2123456/1
type: Range
persistent identity: range2123456
display id: range2123456
version: 1
top level: BBa_K541501
direction: direction/forward
start: 86
end: 91
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2123457
display id: annotation2123457
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123457/range2123457/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K541501/annotation2123457/range2123457/1
type: Range
persistent identity: range2123457
display id: range2123457
version: 1
top level: BBa_K541501
direction: direction/forward
start: 106
end: 117
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2123458
display id: annotation2123458
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123458/range2123458/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K541501/annotation2123458/range2123458/1
type: Range
persistent identity: range2123458
display id: range2123458
version: 1
top level: BBa_K541501
direction: direction/reverse
start: 106
end: 117
orientation: inline

SacB Signal tag
type: SequenceAnnotation
persistent identity: annotation2123460
display id: annotation2123460
version: 1
top level: BBa_K541501
location: https://synbiohub.org/public/igem/BBa_K541501/annotation2123460/range2123460/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K541501/annotation2123460/range2123460/1
type: Range
persistent identity: range2123460
display id: range2123460
version: 1
top level: BBa_K541501
direction: direction/forward
start: 124
end: 219
orientation: inline

BBa_K606061
type: ComponentDefinition
persistent identity: BBa K606061
display id: BBa_K606061
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG Ribosome Binding Site (RBS) for B. subtilis
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:51Z
mutable provenance: SYnthesized RBS because part BBa_K143021 was wrong.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SpoVG Ribosome Binding Site (RBS) for B. subtilis
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: RBS
star: false
creator: Axel S??guret
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SpoVG
sequence: https://synbiohub.org/public/igem/BBa_K606061_sequence/1

SpoVG
type: SequenceAnnotation
persistent identity: annotation2160544
display id: annotation2160544
version: 1
top level: BBa_K606061
location: https://synbiohub.org/public/igem/BBa_K606061/annotation2160544/range2160544/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K606061/annotation2160544/range2160544/1
type: Range
persistent identity: range2160544
display id: range2160544
version: 1
top level: BBa_K606061
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1197015_sequence/1
type: Sequence
persistent identity: BBa K1197015 sequence
display id: BBa_K1197015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggctctgttattagcagtttttgtggtggtcgccgcactggtggcgctctacacttggttccagcgcaaccatagctactggcaacgcaagggcataccctatattccgcccacgccgatcattggcaacaccaaggtggtcttcaagatggagaactcctttgggatgcacctatcggagatatacaatgatccgcggctgaaggacgaggctgtggtgggcatctactccatgaacaagcccggcttgataatacgcgacatagagctgatcaaatccattctgatcaaggacttcaatcggttccacaaccgatacgcccgctgcgatccccatggcgatccattgggctataataacctgttcttcgtcagggatgcccattggaagggcattcgcaccaagctgactcccgttttcaccagcggcaaggtcaagcagatgtacacccttatgcaggagattggaaaggatctggagctggcacttcagaggcgtggagagaagaactctgggagtttcattacggagattaaggagatctgcgctcagttctccacggacagcatagccacgattgcatttggcattcgtgctaacagcctagagaatcccaacgcagagttccgtaactatggacgcaagatgttcaccttcaccgtagcgcgtgccaaggacttctttgtggccttcttcctgcccaagctggtgtcgctgatgcgcatccagttcttcacggcggacttttcccactttatgcgcagcaccattggtcacgttatggaggagcgagagcgatcgggcctgctccgcaatgatctcatagatgtcttggtgagtctgcgcaaagaggcggctgccgagccttcgaagcctcactatgccaagaaccaggacttcctggtggctcaggcgggcgtgttttttacggcgggtttcgagacctcctcctcgaccatgtcttttgccctgtacgagatggccaagcatccagagatgcagaaacgcctgcgcgacgagatcaacgaagctttggtggagggcggtgggtcattgagctacgagaaaatccagtccctggagtatctggccatggtggtggacgaggtgctgcgcatgtatccggtgctgccgttcctggaccgcgagtacgagagcgtggagggacagccagacttgagcctgaagccgttctacgactacactctcgagaacggaacccctgtgttcatacccatctatgcactgcatcatgatccaaagtactggaccaatcccagccaattcgatccggagcgtttctcacccgcgaaccgcaagaacatagtggccatggcatatcaacccttcggatctgggccgcacaactgcattggcagccggattggcctgctacagagcaaactgggactggtcagcctgctgaagaatcactcagtgcgcaactgcgaggccaccatgaaggacatgaaattcgatcccaagggtttcgtgctccaggcagatggtggcatacatttggagatagtcaacgatcgcctctacgatcagagcgctccatcgctccaataataatactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggctgcggaacacgtcgaagaagtggtttcggaagaaccatttttgggcacactggatattgcatta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1197013_sequence/1
type: Sequence
persistent identity: BBa K1197013 sequence
display id: BBa_K1197013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctctgttattagcagtttttgtggtggtcgccgcactggtggcgctctacacttggttccagcgcaaccatagctactggcaacgcaagggcataccctatattccgcccacgccgatcattggcaacaccaaggtggtcttcaagatggagaactcctttgggatgcacctatcggagatatacaatgatccgcggctgaaggacgaggctgtggtgggcatctactccatgaacaagcccggcttgataatacgcgacatagagctgatcaaatccattctgatcaaggacttcaatcggttccacaaccgatacgcccgctgcgatccccatggcgatccattgggctataataacctgttcttcgtcagggatgcccattggaagggcattcgcaccaagctgactcccgttttcaccagcggcaaggtcaagcagatgtacacccttatgcaggagattggaaaggatctggagctggcacttcagaggcgtggagagaagaactctgggagtttcattacggagattaaggagatctgcgctcagttctccacggacagcatagccacgattgcatttggcattcgtgctaacagcctagagaatcccaacgcagagttccgtaactatggacgcaagatgttcaccttcaccgtagcgcgtgccaaggacttctttgtggccttcttcctgcccaagctggtgtcgctgatgcgcatccagttcttcacggcggacttttcccactttatgcgcagcaccattggtcacgttatggaggagcgagagcgatcgggcctgctccgcaatgatctcatagatgtcttggtgagtctgcgcaaagaggcggctgccgagccttcgaagcctcactatgccaagaaccaggacttcctggtggctcaggcgggcgtgttttttacggcgggtttcgagacctcctcctcgaccatgtcttttgccctgtacgagatggccaagcatccagagatgcagaaacgcctgcgcgacgagatcaacgaagctttggtggagggcggtgggtcattgagctacgagaaaatccagtccctggagtatctggccatggtggtggacgaggtgctgcgcatgtatccggtgctgccgttcctggaccgcgagtacgagagcgtggagggacagccagacttgagcctgaagccgttctacgactacactctcgagaacggaacccctgtgttcatacccatctatgcactgcatcatgatccaaagtactggaccaatcccagccaattcgatccggagcgtttctcacccgcgaaccgcaagaacatagtggccatggcatatcaacccttcggatctgggccgcacaactgcattggcagccggattggcctgctacagagcaaactgggactggtcagcctgctgaagaatcactcagtgcgcaactgcgaggccaccatgaaggacatgaaattcgatcccaagggtttcgtgctccaggcagatggtggcatacatttggagatagtcaacgatcgcctctacgatcagagcgctccatcgctccaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1197016_sequence/1
type: Sequence
persistent identity: BBa K1197016 sequence
display id: BBa_K1197016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggctgcggaacacgtcgaagaagtggtttcggaagaaccatttttgggcacactggatattgcattattagtagtcctcttagtcggtgcaacatggtactttatgagaagtcgtaagaaggaagaagctccaattcgttcatattctatacaacccactacagtcagcaccgtttcaacgacagagaactcgttcataaaaaaattgaaagcttcaggtcgtagtttagttgtgttttatggttcccagaccggaacagccgaagaatttgccggacgattggccaaggaaggtttacgctatcgcatgaagggaatggtggcggaccccgaagaatgtgacatggaagaattgttacaaatgaaggatattcctaactcattagccgttttctgtttggctacatatggtgaaggagatcccactgataacgctatggaattttatgaatggattacaaatggcgaggttgatttgacgggtttaaactacgccgtatttggcttaggcaacaagacttatgaacattacaacaaagttgcaatttatgtggacaagaggttggaggaactcggtgctacgagagtgttcgagttaggtcttggagacgatgatgcaaatatcgaagatgacttcattacgtggaaagacagattttggccatctgtttgtgatttcttcggaatcgaaggaagtggtgaggaggtattgatgcgtcaattccgacttttggaacagccggatgttcaacccgatcgtatttacaccggcgaaatagctcggttgcactcaatgcaaaatcaaagacctccatttgatgctaaaaatccatttttggcttcagtcatagttaatcgagagctgcataaaggcggcggacggtcatgtatgcatattgagttggatattgatggatcaaaaatgcggtatgatgcaggcgaccatatagcaatgtatccgattaatgataaaatcctggtagaaaaattgggtaaactatgtgatgcaaatctggatacagtcttctctttgatcaatacggatacggatagcagtaaaaaacatccattcccctgtcccaccacataccgtaccgccctgacacattacttggagattactgcaataccgagaacacatattctgaaggagttagcagagtattgttccgatgaaaaggataaggaatttttacgcaacatggcgtctattactccggaaggcaaagagaagtatcagaattggatccagaactcttcaagaaatattgtgcatattttggaggatatcaaatcttgtagaccaccaattgatcatatttgtgaactgttgccaagacttcaaccgcgttattactcgatttcatcttcttctaagctctatccaactaatgttcatattacagcagttctggttcaatatgaaacacctactggccgtgtaaacaaaggtgttgctacgtcatacatgaaggaaaaaaatccttcggttggagaggttaaagtgcctgtatttattcgcaaatctcaatttaggttgccaactaaatccgagattccaatcataatggtgggcccaggaacgggtctagcacctttcagaggattcatacaggaaagacaatttttacgagatggaggaaaagttgtcggcgataccatattgtatttcgggtgtagaaagaaggacgaagacttcatttatagagaagaactagagcaatatgtacaaaatggaactttaaccttgaaaacggcattttcaagagaccaacaagaaaaaatttatgttactcatctaatcgaacaagatgctgatttaatttggaaagtaattggcgaacaaaagggacacttttatatttgcggggatgctaaaaatatggctgtggatgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K541501_sequence/1
type: Sequence
persistent identity: BBa K541501 sequence
display id: BBa_K541501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K606061_sequence/1
type: Sequence
persistent identity: BBa K606061 sequence
display id: BBa_K606061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z