BBa_K1199011 1 BBa_K1199011 LinA 2013-09-10T11:00:00Z 2015-05-08T01:09:39Z LinA is cofactor-independent dehalogenase from Pseudomonas paucimobilis UT26 LinA mediates gamma-HCH to 1,3,4,6-tetrachloro-1,4-cyclohexadiene false false _1513_ 0 16962 9 In stock false no restriction enzyme site contained false Tian Wang BBa_K199012 1 BBa_K199012 Tet Resistance with CCCUC Addition 2009-06-28T11:00:00Z 2015-05-08T01:11:18Z The template of the gene comes from BioBrick part J31007. We designed a forward primer that included the first 20 nucleotides on the 5' end, and a reverse primer that included the last 20 nucleotides on the 3' end of the Tet resistance gene. This Tet resistance reporter gene has a 5-base pair CCCUC addition after the start codon and before the rest of the gene. This is used in conjunction with the CCCUC tRNA (BioBrick part K199001). This CCCUC tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter. false false _295_ 0 5114 9 It's complicated false We positioned the ATG before the 5-base codon and continued with the rest of the Tet resistance gene. This way, the RBS wouldn't read the reporter gene without reading over the 5-base codon first. In order to ligate our 5-base pair addition at the desired position in the reporter gene, we utilized the restriction enzyme BamHI which cut at a single site 290 bp into the gene. false Leland Taylor annotation2006997 1 BamHI Restriction Site range2006997 1 295 300 annotation2006996 1 CCCUC 5-base pair addition range2006996 1 4 8 BBa_K1199025 1 BBa_K1199025 LinA-RBS-LinB 2013-09-11T11:00:00Z 2015-05-08T01:09:39Z s s false false _1513_ 0 16920 9 Not in stock false a false Yanpu Chen component2339355 1 BBa_K199012 component2339352 1 BBa_K1199011 annotation2339355 1 BBa_K199012 range2339355 1 478 1673 annotation2339352 1 BBa_K1199011 range2339352 1 1 471 BBa_K199012_sequence 1 atgccctcaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa BBa_K1199011_sequence 1 atgtcggatctggatcgcctggcaagtcgtgctgctattcaagacctgtactcggataaactgattgccgtggataagcgtcaagaaggccgtctggcatctatttggtgggatgacgctgaatggacgattgaaggcattggtacgtataaaggcccagaaggcgcgctggatctggctaacaatgtgctgtggccgatgtttcatgaatgcattcactacggcaccaacctgcgtctggaatttgtcagcgccgataaagtgaatggcatcggtgacgttctgctgctgggcaacctggtcgaaggtaatcagtcaattctgatcgcggccgtctttaccgatgaatatgaacgtcgcgacggcgtttggaaattttcgaagcgtaacgcatgtaccaattacttcacgccgctggcgggcattcattttgccccgccgggtatccactttgccccgagtggcgcctaa BBa_K1199025_sequence 1 atgtcggatctggatcgcctggcaagtcgtgctgctattcaagacctgtactcggataaactgattgccgtggataagcgtcaagaaggccgtctggcatctatttggtgggatgacgctgaatggacgattgaaggcattggtacgtataaaggcccagaaggcgcgctggatctggctaacaatgtgctgtggccgatgtttcatgaatgcattcactacggcaccaacctgcgtctggaatttgtcagcgccgataaagtgaatggcatcggtgacgttctgctgctgggcaacctggtcgaaggtaatcagtcaattctgatcgcggccgtctttaccgatgaatatgaacgtcgcgacggcgtttggaaattttcgaagcgtaacgcatgtaccaattacttcacgccgctggcgggcattcattttgccccgccgggtatccactttgccccgagtggcgcctaatactagatgccctcaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z