BBa_K1199025
1
BBa_K1199025
LinA-RBS-LinB
2013-09-11T11:00:00Z
2015-05-08T01:09:39Z
s
s
false
false
_1513_
0
16920
9
Not in stock
false
a
false
Yanpu Chen
component2339352
1
BBa_K1199011
component2339355
1
BBa_K199012
annotation2339355
1
BBa_K199012
range2339355
1
478
1673
annotation2339352
1
BBa_K1199011
range2339352
1
1
471
BBa_K1199011
1
BBa_K1199011
LinA
2013-09-10T11:00:00Z
2015-05-08T01:09:39Z
LinA is cofactor-independent dehalogenase from Pseudomonas paucimobilis UT26
LinA mediates gamma-HCH to 1,3,4,6-tetrachloro-1,4-cyclohexadiene
false
false
_1513_
0
16962
9
In stock
false
no restriction enzyme site contained
false
Tian Wang
BBa_K199012
1
BBa_K199012
Tet Resistance with CCCUC Addition
2009-06-28T11:00:00Z
2015-05-08T01:11:18Z
The template of the gene comes from BioBrick part J31007. We designed a forward primer that included the first 20 nucleotides on the 5' end, and a reverse primer that included the last 20 nucleotides on the 3' end of the Tet resistance gene.
This Tet resistance reporter gene has a 5-base pair CCCUC addition after the start codon and before the rest of the gene. This is used in conjunction with the CCCUC tRNA (BioBrick part K199001). This CCCUC tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter.
false
false
_295_
0
5114
9
It's complicated
false
We positioned the ATG before the 5-base codon and continued with the rest of the Tet resistance gene. This way, the RBS wouldn't read the reporter gene without reading over the 5-base codon first. In order to ligate our 5-base pair addition at the desired position in the reporter gene, we utilized the restriction enzyme BamHI which cut at a single site 290 bp into the gene.
false
Leland Taylor
annotation2006996
1
CCCUC 5-base pair addition
range2006996
1
4
8
annotation2006997
1
BamHI Restriction Site
range2006997
1
295
300
BBa_K199012_sequence
1
atgccctcaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
BBa_K1199011_sequence
1
atgtcggatctggatcgcctggcaagtcgtgctgctattcaagacctgtactcggataaactgattgccgtggataagcgtcaagaaggccgtctggcatctatttggtgggatgacgctgaatggacgattgaaggcattggtacgtataaaggcccagaaggcgcgctggatctggctaacaatgtgctgtggccgatgtttcatgaatgcattcactacggcaccaacctgcgtctggaatttgtcagcgccgataaagtgaatggcatcggtgacgttctgctgctgggcaacctggtcgaaggtaatcagtcaattctgatcgcggccgtctttaccgatgaatatgaacgtcgcgacggcgtttggaaattttcgaagcgtaacgcatgtaccaattacttcacgccgctggcgggcattcattttgccccgccgggtatccactttgccccgagtggcgcctaa
BBa_K1199025_sequence
1
atgtcggatctggatcgcctggcaagtcgtgctgctattcaagacctgtactcggataaactgattgccgtggataagcgtcaagaaggccgtctggcatctatttggtgggatgacgctgaatggacgattgaaggcattggtacgtataaaggcccagaaggcgcgctggatctggctaacaatgtgctgtggccgatgtttcatgaatgcattcactacggcaccaacctgcgtctggaatttgtcagcgccgataaagtgaatggcatcggtgacgttctgctgctgggcaacctggtcgaaggtaatcagtcaattctgatcgcggccgtctttaccgatgaatatgaacgtcgcgacggcgtttggaaattttcgaagcgtaacgcatgtaccaattacttcacgccgctggcgggcattcattttgccccgccgggtatccactttgccccgagtggcgcctaatactagatgccctcaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z