BBa_K1199043 1 BBa_K1199043 DhaA31 TCP(trichlorophenol)->2,3-DCP(2,3-dichloropropano) 2013-09-15T11:00:00Z 2015-05-08T01:09:39Z DhaA is from Rhodococcus sp hydrolyzes The enzyme DhaA is of particular interest because it enables a very slow conversion of the toxic artificial compound and suspected human carcinogen 1,2,3-trichloropropane (TCP) to 2,3-dichloropropane-1-ol under laboratory conditions .And DhaA31 is a mutant of DhaA ,DhaA31 has large aromatic residues at two of the three randomized positions and at two positions modified by site-directed mutagenesis. These changes enhance activity towards TCP by decreasing the accessibility of the active site to water molecules, thus promoting the formation of an activated complex. false false _1513_ 0 16962 9 It's complicated true no restriction site contained false Tian Wang BBa_K1199043_sequence 1 atgtctgaaatcggtacgggttttccgtttgatccgcattatgttgaagtcctgggcgaacgtatgcactacgtggatgttggcccgcgcgacggcaccccggtgctgtttctgcatggcaacccgaccagcagctatctgtggcgtaatattatcccgcatgtggctccgtcacaccgctgcattgcgccggatctgatcggcatgggtaaatcggacaagccggatctggactatttctttgatgaccatgtgcgttacctggatgcttttattgaagcgctgggcctggaagaagtggttctggttatccatgactggggctctgctctgggtttccactgggcgaaacgtaacccggaacgcgtgaagggtattgcctgtatggaatttatccgtccgttcccgacctgggatgaatggccggaatttgcgcgcgaaacctttcaggccttccgtacggcagatgttggccgcgaactgattatcgaccaaaatgccttcattgaaggcgcgctgccgaaatatgtcgtgcgtccgctgaccgaagttgaaatggatcattaccgcgaaccgtttctgaagccggtggatcgtgaaccgctgtggcgcttcccgaacgaactgccgattgctggcgaaccggcgaatatcgtcgccctggtggaagcatatatgaactggctgcaccagagtccggttccgaaactgctgttttggggcacgccgggcttcattatcccgccggccgaagccgcacgtctggcagaatccctgccgaactgcaaaacggtcgatattggcccaggcctgcattttctgcaagaagataatccggatctgattggcagtgaaatcgctcgttggctgccggcgctgcatcaccatcaccatcactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z