BBa_K1202113 1 BBa_K1202113 TAT-RFP 2013-09-23T11:00:00Z 2015-05-08T01:09:40Z tat signal peptide was prepare from Human immunodeficiency Virus-1(HIV-1).RFP sequence was taked parts.igem.org this part designed to tat signal peptide test. false false _1516_ 0 17366 9 It's complicated false Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli. Additionally, a linker peptide coding region is added for the maximum correctness for our experiments. Lastly, a his tag coding region is added for the purification purposes. false safa tapan annotation2369257 1 Start Codon range2369257 1 48 50 annotation2369256 1 RBS range2369256 1 36 47 annotation2369258 1 His Tag range2369258 1 51 68 annotation2369260 1 Linker range2369260 1 105 149 annotation2369259 1 TAT range2369259 1 69 104 annotation2369255 1 J23100 range2369255 1 1 35 annotation2369261 1 RFP range2369261 1 150 830 annotation2369262 1 Stop Codon range2369262 1 825 830 BBa_K1202113_sequence 1 ttgacggctagctcagtcctaggtacagtgctagcaaagaggagaaaatgcatcaccatcatcaccatatgtatggtcgtaaaaaacgtcgtcagcgtcgtcgtggtggtggtggcagcggtggcggtggtagtggcggtggcggtagcatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z