BBa_K1204013
1
BBa_K1204013
TEF1pr-BS1x-16A
2013-09-15T11:00:00Z
2015-05-08T01:09:40Z
The binding site was synthetically designed using the binding rules for iTALs and gRNA/dCas9 complex and ordered as a gene block from IDT. The TEF1 promoter is a strong constitutive promoter in yeast that we got from another plasmid in the lab.
This is a strong constitutive promoter in yeast with a binding site downstream. The binding site is designed to be directly targeted by our iTAL 16A or gRNA 16A. When the binding site is occupied by an iTAL or gRNA/dCas9 complex, the expression of the YFP reporter should be knocked down.
false
false
_1518_
0
17071
9
It's complicated
false
We had to consider the manner in which iTALs and gRNA/dCas9 complex bind in designing our sequence.
false
Matthew Baron
BBa_K1204013_sequence
1
cgcgccctcatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaaattttttttttgatttttttctctttcgatgacctcccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagttttcttctagtagtaaaagtcccttctgtacagtgacgctggtagtcgaaatcggatagctagaccgaaaagtagctgttcaccatcacctgaacacccagtcagcgagcacgctagcaactgaaggcctctgtccagtatagtgcacta
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z