BBa_K1207000 1 BBa_K1207000 Gram positive origin of replication 2013-09-11T11:00:00Z 2015-05-08T01:09:40Z This part was cloned from the plasmid pIKM1. Its original source was B. subtilis. This part is an origin of replication for Gram positive organisms. This sequence has been used in plasmids for electrotransformation of Clostridia with success. It is necessary to have a functional origin of replication for the chassis organism; therefore, if the chassis organism used is a Gram positive, this sequence will be a necessary addition to the iGEM standard plasmid backbones in order to allow for the replication of the plasmid. false false _1521_1241_ 0 14319 9 It's complicated false The cloning of this part was relatively straight-forward. No special considerations were required. false Jeremiah Miller BBa_K1207000_sequence 1 cctgatatgctccgacgctttatatagaaaagaagattcaactaggtaaaatcttaatataggttgagatgataaggtttataaggaatttgtttgttctaatttttcactcattttgttctaatttcttttaacaaatgttcttttttttttagaacagttatgatatagttagaatagtttaaaataaggagtgagaaaaagatgaaagaaagatatggaacagtctataaaggctctcagaggctcatagacgaagaaagtggagaagtcatagaggtagacaagttataccgtaaacaaacgtctggtaacttcgtaaaggcatatatagtgcaattaataagtatgttagatatgattggcggaaaaaaacttaaaatcgttaactatatcctagataatgtccacttaagtaacaatacaatgatagctacaacaagagaaatagcaaaagctacaggaacaagtctacaaacagtaataacaacacttaaaatcttagaagaaggaaatattataaaaagaaaaactggagtattaatgttaaaccctgaactactaatgagaggcgacgaccaaaaacaaaaatacctcttactcgaatttgggaactttgagcaagaggcaaatgaaatagattgacctcccaataacaccacgtagttattgggaggtcaatctatgaaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z