LOCUS       BBa_K1210000            1927 bp    DNA     linear       22-Nov-2024
DEFINITION  LacI-CFP-PK401-YFP
ACCESSION   BBa_K1210000
VERSION     BBa_K1210000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             221..943
                     /label=BBa_E0020
     misc_feature    944..1036
                     /label=BBa_K1210001
     CDS             1037..1759
                     /label=BBa_E0030
     stem_loop       1771..1814
                     /label=stem_loop
     misc_feature    1760..1839
                     /label=BBa_B0010
     terminator      1760..1839
                     /label=BBa_B0010
     stem_loop       1859..1902
                     /label=stem_loop
     misc_feature    1848..1927
                     /label=BBa_B0010
     terminator      1848..1927
                     /label=BBa_B0010
     terminator      1760..1927
                     /label=BBa_B0017
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atggtgagca agggcgagga
      241 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa
      301 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      361 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg
      421 gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      481 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      541 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      601 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat
      661 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
      721 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      781 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      841 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      901 cgccgggatc actctcggca tggacgagct gtacaagtaa taaagctttt taaagcagaa
      961 agcgcgcgca cggagcgtgg cgtgcgcgcg caagcaagtg gatgtgatcc tgatgttgaa
     1021 aagccacgct tgggccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1081 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1141 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1201 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta
     1261 ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1321 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1381 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1441 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1501 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1561 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1621 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     1681 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1741 cgagctgtac aagtaataac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1801 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagcca ggcatcaaat
     1861 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1921 cgctctc
//