BBa_K1210003 1 BBa_K1210003 PK401 tagged dual coding test construct 2013-10-14T11:00:00Z 2015-05-08T01:09:40Z This part was synthesized by Genewiz. It contains genomic sequence from IBV virus (PK401 pseudoknot; non-infectious sequence), and from IAPV virusb(IAPV overlapping ORFs; only 7 aa used of the overlapping genes to avoid producing anything harmful). Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Includes a His-tagged CFP before the PK401 pseudoknot, and two different polypeptides in the 0 and -1 reading frames with different protein tags. If translation is halted at the pseudoknot due to the inability to melt the mRNA secondary structure, a 27.7 kDa protein is produced of just CFP-His. In the 0 frame if read-through of the pseudoknot occurs, a fragment of the IAPV dual coded gene along with a strep-tag II will be translated creating a 33.3 kDa protein of CFP-His-PK401-IAPV-StrepII. In the -1 frame if read-through of the pseudoknot occurs, a fragment containing the second dual coded IAPV gene along with a GST tag is produced. This contains CFP-His-PK401-IAPV2-GST, a 60.9 kDa protein. false false _1524_ 0 6050 9 It's complicated false This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios. false Harland Brandon BBa_K1210003_sequence 1 taatacgactcactataggggaattgtgagcggataacaattccccactagaaataattttgtttaactttaagaaggagatataccatggtctcaaaaggggaagaactgttcaccggcgtcgttcctatcctggttgagctggacggtgatgtgaatggccataaattctccgtatctggagaaggcgaaggtgatgctacctacggtaaattgaccctgaagttcatctgtaccactggcaaacttcctgtcccgtggcctactctggtaaccacgttaacgtggggagtacagtgtttttcacgctatccggatcatatgaaacagcacgatttttttaaatctgctatgcccgaaggttatgtccaggagcggaccattttttttaaagacgatggaaactacaaaacccgggctgaggtcaaatttgagggcgacacattagtcaatcgtattgagctgaaagggatcgattttaaggaggatggtaacatcctgggtcacaagctggagtataattacatttctcataatgtttacattaccgccgacaagcaaaaaaatggcattaaggccaatttcaaaatccgtcataacattgaggatggtagcgtgcagcttgccgaccactatcaacagaatacgccgattggtgatggcccggtattgttaccggataaccactatctgagcactcagtctgcattgtccaaagatccgaatgaaaaacgcgaccatatggtgctgctggagtttgtgacggcggcgggtatcactctgggcatggatgaactgtataagcaccaccaccaccaccacagctttttaaagcagaaagcgcgcgcacggagcgtggcgtgcgcgcgcaagcaagtggatgtgatcctgatgttgaaaagccacgcttgggccatgatgtggaaactccaaatctggagccatccgcagttcgaaaaatgatgtcgccaattttggggtactggaaaatcaagggtcttgtccaaccgacccgtctcttactggaatacctggaagagaaatacgaagaacatctgtacgaacgcgacgaaggggataaatggcgcaacaagaaatttgagctgggcttagagtttcctaacctcccgtattatattgacggtgatgttaaactcacacaaagtatggcaatcatccgttacatcgcagacaaacataacatgttaggtggttgcccgaaagagcgtgctgaaatcagtatgctggagggggctgtgcttgatatccgctatggcgtctcacgcattgcatacagtaaagacttcgagacgctgaaggtcgactttttatcaaaattaccggaaatgcttaaaatgtttgaagatcgcctttgccacaaaacctatctgaatggtgatcacgtcacgcatcctgattttatgctgtatgatgctttggatgtggtgttatatatggatcctatgtgtctcgatgccttccccaaactggtgtgtttcaaaaaacgcattgaagcaattccgcaaattgataagtacttaaaatctagtaaatacatcgcatggccgctgcaagggtggcaagcaacgtttggtgggggagaccacccgccgaagtctgacttagtccctcgtggtagcccggaatttccgggtcgccttgagcggcctcaccgtgattaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z