LOCUS       BBa_K121017              927 bp    DNA     linear       22-Nov-2024
DEFINITION  promoterless gfp
ACCESSION   BBa_K121017
VERSION     BBa_K121017.1  
FEATURES             Location/Qualifiers
     RBS             50..64
                     /label=B0030
     misc_feature    1..6
                     /label=Sal I
     misc_feature    15..20
                     /label=Bam HI
     misc_feature    26..31
                     /label=Bgl II
     misc_feature    41..46
                     /label=Mlu I
     RBS             1..64
                     /label=BBa_K121016
     CDS             71..790
                     /label=GFP protein
     CDS             71..790
                     /label=BBa_E0040
     stem_loop       810..853
                     /label=stem_loop
     misc_feature    799..878
                     /label=BBa_B0010
     terminator      799..878
                     /label=BBa_B0010
     stem_loop       894..913
                     /label=T7 TE
     misc_feature    920..920
                     /label=stop
     polyA_site      914..927
                     /label=polya
     misc_feature    887..927
                     /label=BBa_B0012
     terminator      887..927
                     /label=BBa_B0012
ORIGIN
        1 gtcgactgac gactggatcc tgtcggagat ctgtcggata acgcgtgaga ttaaagagga
       61 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      121 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      181 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      241 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      301 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      361 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      421 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      481 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      541 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      601 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      661 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
      721 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
      781 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      841 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      901 cttcgggtgg gcctttctgc gtttata
//