LOCUS       BBa_K121097             1167 bp    DNA     linear       15-Nov-2024
DEFINITION  test
ACCESSION   BBa_K121097
VERSION     BBa_K121097.1  
FEATURES             Location/Qualifiers
     misc_feature    1..232
                     /label=K121011
     misc_feature    94..131
                     /label=CAP binding site
     promoter        141..146
                     /label=-35
     promoter        165..168
                     /label=-10
     misc_feature    169..203
                     /label=LacI binding site
     promoter        1..232
                     /label=BBa_K121011
     RBS             290..304
                     /label=B0030
     misc_feature    241..246
                     /label=Sal I
     misc_feature    255..260
                     /label=Bam HI
     misc_feature    266..271
                     /label=Bgl II
     misc_feature    281..286
                     /label=Mlu I
     RBS             241..304
                     /label=BBa_K121016
     CDS             311..1030
                     /label=GFP protein
     CDS             311..1030
                     /label=BBa_E0040
     stem_loop       1050..1093
                     /label=stem_loop
     misc_feature    1039..1118
                     /label=BBa_B0010
     terminator      1039..1118
                     /label=BBa_B0010
     stem_loop       1134..1153
                     /label=T7 TE
     misc_feature    1160..1160
                     /label=stop
     polyA_site      1154..1167
                     /label=polya
     misc_feature    1127..1167
                     /label=BBa_B0012
     terminator      1127..1167
                     /label=BBa_B0012
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg cctactagag
      241 gtcgactgac gactggatcc tgtcggagat ctgtcggata acgcgtgaga ttaaagagga
      301 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      361 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      421 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      481 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      541 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      601 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      661 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      721 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      781 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      841 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      901 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
      961 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1021 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1081 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1141 cttcgggtgg gcctttctgc gtttata
//