LOCUS BBa_K1211003 4432 bp DNA linear 23-Dec-2024 DEFINITION phaC1-A-B1 [P(3HB) synthesis] under pBAD ACCESSION BBa_K1211003 VERSION BBa_K1211003.1 FEATURES Location/Qualifiers promoter 1..274 /label=pBAD RBS 560..566 /label=RBS CDS 577..2346 /label=phaC1 RBS 2420..2423 /label=RBS CDS 2431..3612 /label=phaA RBS 3677..3680 /label=RBS CDS 3687..4427 /label=phaB1 misc_feature 1..4432 /label=BBa_K934001 ORIGIN 1 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc 61 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa 121 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat 181 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat 241 cctacctgac gctttttatc gcaactctct actggtctcg gaatcgctga cgattcccag 301 gtttctccgg caagcatagc gcatggcgtc tccatgcgag aatgtcgcgc ttgccggata 361 aaaggggagc cgctatcgga atggacgcaa gccacggccg cagcaggtgc ggtcgagggc 421 ttccagccag ttccagggca gatgtgccgg cagaccctcc cgctttgggg gaggcgcaag 481 ccgggtccat tcggatagca tctccccatg caaagtgccg gccagggcaa tgcccggagc 541 cggttcgaat agtgacggca gagagacaat caaatcatgg ctactgggaa aggagccgcc 601 gccagcaccc aggaaggcaa aagccaacca tttaaagtga ccccaggccc gttcgatcct 661 gccacctggc tggaatggtc acgtcaatgg caaggtacgg agggcaacgg ccatgcggca 721 gcgtccggca tccctgggct tgatgcactg gctggtgtta aaattgcgcc tgcccaattg 781 ggagacatcc aacagcgtta tatgaaagat ttttcggcgc tgtggcaggc gatggcggaa 841 ggtaaagcag aggcgacggg accgctgcat gaccgtcgct ttgctggcga cgcctggcgt 901 accaatttgc cctatcgttt tgcggcagcc ttctacctgc tgaacgctcg tgccctgact 961 gagctggcag atgctgtcga agcggatgcc aaaacccgcc aacgcatccg ttttgccatc 1021 tcacagtggg tcgacgcaat gtcaccggcc aacttcctgg ccaccaatcc ggaagcacag 1081 cgcctcctga ttgaaagcgg cggggaatcg ctgcgcgccg gcgttcgtaa catgatggaa 1141 gatctgaccc gtggtaagat ttcgcagacg gatgagtcgg ccttcgaggt gggtcgcaat 1201 gttgcagtga cagaaggcgc ggtggtgttt gagaacgaat actttcaact tctccagtat 1261 aaaccactga cggataaagt gcatgcgcgc ccattgttaa tggttccccc gtgcattaat 1321 aaatactata tcctggatct tcagccggaa agctccttag tgcgccacgt ggtggaacag 1381 gggcatactg tgtttttagt tagctggcgc aatccagatg cctcaatggc cggcagtacc 1441 tgggatgact acattgaaca cgcggctatt cgcgcgatcg aagtggcacg cgacatctca 1501 ggtcaggaca agattaacgt actcggcttt tgcgtcggtg gcactatcgt gagcaccgct 1561 ctggcggtac tggctgcgcg tggtgaacac cccgcagcgt cggttacgct tctgaccaca 1621 ctgttggatt tcgccgacac tggcatcttg gatgtatttg tcgatgaggg ccacgttcag 1681 ctgcgcgagg ccactctggg tggcggcgcc ggcgcaccgt gcgctctgct gcgcggtctg 1741 gagctcgcga atactttttc tttcttacgc ccgaacgatc ttgtctggaa ttacgtagtt 1801 gacaactatc tcaaaggtaa cacgcccgtg ccgtttgatt tattattctg gaatggggat 1861 gcgaccaacc tgccgggtcc ttggtattgt tggtatctcc gtcatacata cttacagaat 1921 gaattaaagg tcccaggtaa acttactgtg tgtggtgtcc cggtagatct ggcgagtatc 1981 gacgtgccga cctacattta tgggagtcgt gaagatcata ttgtgccgtg gaccgccgcg 2041 tacgcttcta ccgcgcttct ggcaaacaaa ttacgtttcg ttctgggtgc cagcggccac 2101 attgcgggtg ttattaatcc tccggccaaa aataaacgct cgcattggac aaacgacgcc 2161 ttgccggaga gccctcagca gtggttggcg ggtgccattg agcaccatgg aagttggtgg 2221 ccggactgga cagcctggct ggcgggccaa gcaggcgcaa aacgcgctgc gccagccaac 2281 tatggcaacg cgcgctaccg cgcgattgaa cccgcgcctg gccgctatgt aaaagcaaaa 2341 gcgtgacgct tgcatgagtg ccggcgtgcg tcatgcacgg cgccggcagg cctgcacgtt 2401 ccctcccgtt tccattgaaa ggactacaca atgaccgatg ttgttatcgt cagtgccgcg 2461 cgcaccgccg tgggtaaatt tggcggcagt ctggcgaaaa tcccggcgcc agaattgggc 2521 gcggtggtaa tcaaagcggc cctggaacgc gccggtgtga aacctgagca ggtgagcgaa 2581 gtaattatgg gtcaggttct gaccgcgggc agcgggcaaa atccggcgcg tcaggctgcg 2641 attaaggcag gccttccggc gatggtgccc gcgatgacca ttaacaaagt atgcggatcg 2701 ggactgaaag cagtgatgct ggccgctaac gccattatgg caggcgacgc ggaaattgtg 2761 gtcgctggcg gacaggagaa tatgtcagcc gcgcctcatg tgctgccggg tagccgcgac 2821 ggctttcgca tgggtgatgc aaaactggtc gatactatga ttgttgacgg cttatgggat 2881 gtgtacaacc agtaccacat gggcatcacc gccgagaacg tggccaagga atacggcatc 2941 acacgcgagg cgcaggatga gttcgccgtc ggctcgcaga acaaggccga agccgcgcag 3001 aaggccggca tgtttgacga agagatcgtc ccggtgctga tcccgcagcg caagggcgac 3061 ccggtggcct tcaagaccga cgagttcgtg cgccagggcg ccacgctgga cagcatgtcc 3121 ggcctcaagc ccgccttcga caaggccggc acggtgaccg cggccaacgc ctcgggcctg 3181 aacgacggcg ccgccgcggt ggtggtgatg tcggcggcca aggccaagga actgggcctg 3241 accccgctgg ccacgatcaa gagctatgcc aacgccggtg tcgatcccaa ggtgatgggc 3301 atgggcccgg tgccggcctc caagcgcgcc ctgtcgcgcg ccgagtggac cccgcaagac 3361 ctggacctga tggagatcaa cgaggccttt gccgcgcagg cgctggcggt gcaccagcag 3421 atgggctggg acacctccaa ggtcaatgtg aacggcggcg ccatcgccat cggccacccg 3481 atcggcgcgt cgggctgccg tatcctggtg acgctgctgc acgagatgaa gcgccgtgac 3541 gcgaagaagg gcctggcctc gctgtgcatc ggcggcggca tgggcgtggc gctggcagtc 3601 gagcgcaaat aaggaagggg ttttccgggg ccgcgcgcgg ttggcgcgga cccggcgacg 3661 ataacgaagc caatcaagga gtggacatga ctcagcgcat tgcgtatgtg accggcggca 3721 tgggtggtat cggaaccgcc atttgccagc ggctggccaa ggatggcttt cgtgtggtgg 3781 ccggttgcgg ccccaactcg ccgcgccgcg aaaagtggct ggagcagcag aaggccctgg 3841 gcttcgattt cattgcctcg gaaggcaatg tggctgactg ggactcgacc aagaccgcat 3901 tcgacaaggt caagtccgag gtcggcgagg ttgatgtgct gatcaacaac gccggtatca 3961 cccgcgacgt ggtgttccgc aagatgaccc gcgccgactg ggatgcggtg atcgacacca 4021 acctgacctc gctgttcaac gtcaccaagc aggtgatcga cggcatggcc gaccgtggct 4081 ggggccgcat cgtcaacatc tcgtcggtga acgggcagaa gggccagttc ggccagacca 4141 actactccac cgccaaggcc ggcctgcatg gcttcaccat ggcactggcg caggaagtgg 4201 cgaccaaggg cgtgaccgtc aacacggtct ctccgggcta tatcgccacc gacatggtca 4261 aggcgatccg ccaggacgtg ctcgacaaga tcgtcgcgac gatcccggtc aagcgcctgg 4321 gcctgccgga agagatcgcc tcgatctgcg cctggttgtc gtcggaggag tccggtttct 4381 cgaccggcgc cgacttctcg ctcaacggcg gcctgcatat gggctgacct gc //