LOCUS       BBa_K1212002            2750 bp    DNA     linear       29-Nov-2024
DEFINITION  Golden Gate Compatible pSB3K3
ACCESSION   BBa_K1212002
VERSION     BBa_K1212002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Suffix
     stem_loop       30..64
                     /label=terminator stem loop
     misc_feature    22..93
                     /label=E. coli his operon terminator
     misc_feature    34..35
                     /label=deletion of two bases
     primer_bind     157..176
                     /label=VR
     CDS             239..1054
                     /label=kanR (rev)
     CDS             1386..2210
                     /label=p15A ORI (rev)
     primer_bind     2611..2630
                     /label=VF2
     misc_feature    2498..2503
                     /label=Fixed BsaI Site
     misc_feature    2687..2690
                     /label=end of terminator
     stem_loop       2700..2719
                     /label=terminator stem loop
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct
       61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg
      181 agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
      241 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
      301 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
      361 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
      421 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
      481 aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc
      541 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
      601 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
      661 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
      721 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
      781 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
      841 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
      901 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
      961 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     1021 tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1081 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1141 gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     1201 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     1261 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
     1321 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
     1381 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     1441 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     1501 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     1561 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     1621 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     1681 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1801 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     1861 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     1921 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     1981 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     2041 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     2101 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     2161 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcaag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa accattatta
     2641 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2701 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
//