BBa_K1212009 1 BBa_K1212009 pBAD+Riboswitch2+GFP 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z pBAD and GFP are from the iGEM distribution kit plates and the riboswitch is from the Gallivan Lab's paper A flow cytometry-based screen for synthetic riboswitches. by Lynch et al. in Appl Environ Microbiol. Testing construct. GFP expression is controlled by arabinose and theophylline via the pBAD promoter and the theophylline riboswitch. false false _1526_ 0 18049 9 It's complicated false The end of the riboswitch sequence must be directly before the start codon of the coding sequence for GFP. There cannot be a scar. false Amy Soon component2348116 1 BBa_K1212019 component2348109 1 BBa_K1212000 component2348108 1 BBa_K206000 annotation2348108 1 BBa_K206000 range2348108 1 1 130 annotation2348116 1 BBa_K1212019 range2348116 1 187 1086 annotation2348109 1 BBa_K1212000 range2348109 1 131 186 BBa_K1212000 1 BBa_K1212000 Theophylline Riboswitch (Clone 8.1*) 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Nucleic Acids Res. 2009 January; 37(1): 184???192. Synthetic riboswitch screened from a library of randomized mutants created by cloning theophylline aptamers upstream of the RBS of a B-galactosidase reporter gene where the distance between the aptamer and the RBS was variable. Codes for an RNA secondary structure that, in the presence of theophylline, undergoes a conformational change that makes the previously sequestered ribosome binding site available for binding thus allowing for transcription of the downstream sequence. Nominal induction levels for theophylline are 0 to 10 mM. Achieves an 59 - fold activation ratio in 1 mM theophylline as reported by Gallivan and Lynch in 'A flow cytometry based screen for synthetic riboswitches.' Nucleic Acids Res. 2009 January; 37(1): 184???192. false false _1526_ 0 18052 9 Not in stock false None. false Aura Ferreiro annotation2363692 1 Theophylline aptamer range2363692 1 1 38 annotation2363690 1 RBS/Shine Dalgarno range2363690 1 46 49 BBa_K1212008 1 BBa_K1212008 Golden Gate compatible GFP 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z DNA from iGEM 2013 distribution K145015 with LVA tag fixed for Golden Gate compatibility through site directed mutagenesis. false false _1526_ 0 18052 9 Not in stock false - false Aura Ferreiro annotation2347651 1 LVA tag range2347651 1 717 759 annotation2347666 1 Bsa I fix range2347666 1 648 648 BBa_K1212018 1 BBa_K1212018 B0015 terminator 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution kit plate B0015 terminator with overhang used in our Golden Gate Assembly. false false _1526_ 0 18049 9 It's complicated false . false Amy Soon annotation2348074 1 B0015 range2348074 1 1 129 annotation2348075 1 Golden Gate Assembly Site range2348075 1 130 133 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049254 1 AraI2 range2049254 1 61 78 BBa_K1212019 1 BBa_K1212019 GFP+LVA tag + B0015 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution kit plate GFP+LVA tag + B0015 with BsaI site removed and Golden Gate Assembly site used in our construction. false false _1526_ 0 18049 9 It's complicated false . false Amy Soon component2348089 1 BBa_K1212018 component2348086 1 BBa_K1212008 annotation2348089 1 BBa_K1212018 range2348089 1 768 900 annotation2348086 1 BBa_K1212008 range2348086 1 1 759 BBa_K1212000_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaggacaacaag BBa_K1212018_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212008_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa BBa_K1212019_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212009_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaggacaacaagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z