BBa_K1212004 1 BBa_K1212004 TAL repressor 1 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Assembled in the Segal Lab at the UC Davis Genome Center. See Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects by Meckler et al. submitted to Nucleic Acids Research. Transcription activator-like (TAL) repressor DNA binding protein that binds to a specific DNA sequence, called TALe Binding Site 1 (TBS 1) (part BBa_K1212003). Low binding affinity. false false _1526_ 0 18049 9 Not in stock false . false Amy Soon annotation2347978 1 Golden Gate Assembly Scar range2347978 1 2413 2416 annotation2348015 1 TAL repressor range2348015 1 1 2412 BBa_K1212000 1 BBa_K1212000 Theophylline Riboswitch (Clone 8.1*) 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Nucleic Acids Res. 2009 January; 37(1): 184???192. Synthetic riboswitch screened from a library of randomized mutants created by cloning theophylline aptamers upstream of the RBS of a B-galactosidase reporter gene where the distance between the aptamer and the RBS was variable. Codes for an RNA secondary structure that, in the presence of theophylline, undergoes a conformational change that makes the previously sequestered ribosome binding site available for binding thus allowing for transcription of the downstream sequence. Nominal induction levels for theophylline are 0 to 10 mM. Achieves an 59 - fold activation ratio in 1 mM theophylline as reported by Gallivan and Lynch in 'A flow cytometry based screen for synthetic riboswitches.' Nucleic Acids Res. 2009 January; 37(1): 184???192. false false _1526_ 0 18052 9 Not in stock false None. false Aura Ferreiro annotation2363692 1 Theophylline aptamer range2363692 1 1 38 annotation2363690 1 RBS/Shine Dalgarno range2363690 1 46 49 BBa_K1212011 1 BBa_K1212011 pBAD+Riboswitch2+TAL1 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z The pBAD is a part from the registry. The riboswitch is from the Gallivan Lab's paper A flow cytometry-based screen for synthetic riboswitches. by Lynch et al. in Nucleic Acids Res. The TAL repressor is from the Segal Lab at the UC Davis Genome Center. See their paper Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects. by Meckler et al. submitted to Nucleic Acids Res. TAL repressor 1 expression under the control of a pBAD promoter and a theophylline riboswitch inducible by arabinose and theophylline. false false _1526_ 0 18049 9 It's complicated false There cannot be a scar between the end of the riboswitch and the start codon of the TAL repressor. false Amy Soon component2373131 1 BBa_K1212000 component2373128 1 BBa_K206000 component2373134 1 BBa_K1212004 annotation2373134 1 BBa_K1212004 range2373134 1 201 2616 annotation2373128 1 BBa_K206000 range2373128 1 1 130 annotation2373131 1 BBa_K1212000 range2373131 1 139 194 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_K1212000_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaggacaacaag BBa_K1212011_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaggacaacaagtactagatggatccctccgacgcttcgccggccgcgcaggtggatctacgcacgctcggctacagtcagcagcagcaagagaagatcaaaccgaaggtgcgttcgacagtggcgcagcaccacgaggcactggtgggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacgtatcagcacataatcacggcgttgccagaggcgacacacgaagacatcgttggcgtcggcaaacagtggtccggcgcacgcgccctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaaacgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttacgccgcagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataaggggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgcaagcaatggcggtggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggcaatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcaacaggtggtagccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtagcgatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatggcggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataagggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacgggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtggccatcgccagcaatattggcggcaggccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgccatggatgcagtgaaaaagggactgccgcacgcgccggaattgatcagaagagtcaatcgccgtccggatcctaccggttgagctt BBa_K1212004_sequence 1 atggatccctccgacgcttcgccggccgcgcaggtggatctacgcacgctcggctacagtcagcagcagcaagagaagatcaaaccgaaggtgcgttcgacagtggcgcagcaccacgaggcactggtgggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacgtatcagcacataatcacggcgttgccagaggcgacacacgaagacatcgttggcgtcggcaaacagtggtccggcgcacgcgccctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaaacgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttacgccgcagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataaggggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgcaagcaatggcggtggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggcaatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcaacaggtggtagccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtagcgatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatggcggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataagggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacgggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtggccatcgccagcaatattggcggcaggccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgccatggatgcagtgaaaaagggactgccgcacgcgccggaattgatcagaagagtcaatcgccgtccggatcctaccggttgagctt BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z