LOCUS       BBa_K1212012            2616 bp    DNA     linear       27-Dec-2024
DEFINITION  pBAD+Riboswitch2+TAL8
ACCESSION   BBa_K1212012
VERSION     BBa_K1212012.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     RBS             184..187
                     /label=RBS/Shine Dalgarno
     stem_loop       139..176
                     /label=Theophylline aptamer
     misc_feature    139..194
                     /label=BBa_K1212000
     CDS             201..2612
                     /label=TAL repressor
     misc_feature    2613..2616
                     /label=Golden Gate Assembly site
     CDS             201..2616
                     /label=BBa_K1212007
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagaggg tgataccagc atcgtcttga tgcccttggc agcaccccgc
      181 tgcaggacaa caagtactag atggatccct ccgacgcttc gccggccgcg caggtggatc
      241 tacgcacgct cggctacagt cagcagcagc aagagaagat caaaccgaag gtgcgttcga
      301 cagtggcgca gcaccacgag gcactggtgg gccatgggtt tacacacgcg cacatcgttg
      361 cgctcagcca acacccggca gcgttaggga ccgtcgctgt cacgtatcag cacataatca
      421 cggcgttgcc agaggcgaca cacgaagaca tcgttggcgt cggcaaacag tggtccggcg
      481 cacgcgccct ggaggccttg ctcacggtgg cgggagagtt gagaggtcca ccgttacagt
      541 tggacacagg ccaacttctc aagattgcaa aacgtggcgg cgtgaccgca gtggaggcag
      601 tgcatgcatg gcgcaatgca ctgacgggtg cccccctgaa ccttacgccg cagcaggtgg
      661 tggccatcgc cagcaataat ggtggcaagc aggcgctgga gacggtgcag cggctgcttc
      721 cggtgctgtg ccaggcccat ggcctgaccc cggagcaggt ggtggccatc gccagcaata
      781 ttggtggcaa gcaggcgctg gagacggtgc agcgattgtt gccggtgctg tgccaggccc
      841 atggcctgac cccggagcag gtggtggcca tcgccagcaa taatggtggc aagcaggcgc
      901 tggagactgt ccagcggctg ttgccggtgc tgtgccaggc ccatggcctg accccggagc
      961 aggtggtggc catcgccagc aatggcggtg gcaagcaggc gcttgagacg gtgcagcggc
     1021 tgttgccggt gctgtgccag gcccatggcc tgaccccgca gcaggtggtg gccatcgcca
     1081 gcaataatgg tggcaagcag gctctggaga cggtgcagcg gctgttgccg gtgctgtgcc
     1141 aggcccatgg cctgaccccg gagcaggtgg tggccatcgc cagccacgac gggggcaagc
     1201 aggcgctgga gacggtgcag cggctgttgc cggtgctgtg ccaggcccat ggcctgaccc
     1261 cgcagcaggt ggtggccatc gccagcaata atggcggcaa gcaggcgctg gagacggtgc
     1321 aggcgctgtt gccggtgctg tgccaggccc atggcctgac cccggagcag gtggtggcca
     1381 tcgcaagcaa taagggtggc aagcaggcgc tggagacggt gcaggcgctg ttgccggtgc
     1441 tgtgccaggc ccatggcctg accccggagc aggtggtggc aatcgccagc aataatggtg
     1501 gcaagcaggc gctggagacg gtgcagcggc tgttgccggt gctgtgccag gcccatggcc
     1561 tgaccccgca acaggtggta gccatcgcca gcaatattgg tggcaagcag gcgctggaga
     1621 cggtgcagcg gctgttgccg gtgctgtgcc aggcccatgg cctgacaccc cagcaggtgg
     1681 tagcgatcgc cagcaataat ggtggcaagc aggcgctgga gacggtgcag cggctgcttc
     1741 cggtgctgtg ccaggcccat ggcctgaccc cggagcaggt ggtggccatc gccagccatg
     1801 atggcggcaa gcaggcgctg gagacggtgc agcgattgtt gccggtgctg tgccaggccc
     1861 atggcctgac cccggagcag gtggtggcca tcgccagcaa taatggtggc aagcaggcgc
     1921 tggagactgt ccagcggctg ttgccggtgc tgtgccaggc ccatggcctg accccggagc
     1981 aggtggtggc catcgccagc aatggcggtg gcaagcaggc gcttgagacg gtgcagcggc
     2041 tgttgccggt gctgtgccag gcccatggcc tgaccccgca gcaggtggtg gccatcgcca
     2101 gcaataatgg tggcaagcag gctctggaga cggtgcagcg gctgttgccg gtgctgtgcc
     2161 aggcccatgg cctgaccccg gagcaggtgg tggccatcgc cagcaataag gggggcaagc
     2221 aggcgctgga gacggtgcag cggctgttgc cggtgctgtg ccaggcccat ggcctgaccc
     2281 cgcagcaggt ggtggccatc gccagcaata atggcggcaa gcaggcgctg gagacggtgc
     2341 aggcgctgtt gccggtgctg tgccaggccc atggcctgac accccagcag gtggtggcca
     2401 tcgccagcaa taatggcggc aggccggcgc tggagagcat tgttgcccag ttatctcgcc
     2461 ctgatccggc gttggccgcg ttgaccaacg accacctcgt cgccttggcc tgcctcggcg
     2521 gacgtcctgc catggatgca gtgaaaaagg gactgccgca cgcgccggaa ttgatcagaa
     2581 gagtcaatcg ccgtccggat cctaccggtt gagctt
//