BBa_K1212001 1 BBa_K1212001 Theophylline Riboswitch (Clone E) 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884. Synthetic riboswitch generated through rational design and in vivo screening. Encodes a RNA secondary structure that in the presence of theophylline undergoes a conformational change that makes available the previously sequestered RBS, thus allowing transcription of the downstream sequence. Achieves a 60-fold activation ratio in 2mM theophylline as reported by Topp et al. in: 'Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species.' Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884 false false _1526_ 0 18052 9 Not in stock false None. false Aura Ferreiro annotation2363694 1 Theophylline aptamer range2363694 1 1 38 annotation2363695 1 RBS range2363695 1 44 51 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_K1212004 1 BBa_K1212004 TAL repressor 1 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Assembled in the Segal Lab at the UC Davis Genome Center. See Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects by Meckler et al. submitted to Nucleic Acids Research. Transcription activator-like (TAL) repressor DNA binding protein that binds to a specific DNA sequence, called TALe Binding Site 1 (TBS 1) (part BBa_K1212003). Low binding affinity. false false _1526_ 0 18049 9 Not in stock false . false Amy Soon annotation2348015 1 TAL repressor range2348015 1 1 2412 annotation2347978 1 Golden Gate Assembly Scar range2347978 1 2413 2416 BBa_K1212014 1 BBa_K1212014 pBAD+Riboswitch1+TAL1 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z The pBAD is a part from the registry. The riboswitch is from the Gallivan Lab's paper Synthetic riboswitches that induce gene expression in diverse bacterial species. by Topp et al. in Appl Environ Microbiol. The TAL repressor is from the Segal Lab at the UC Davis Genome Center. See their paper Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects. by Meckler et al. submitted to Nucleic Acids Res. TAL repressor 1 expression under the control of a pBAD promoter and a theophylline riboswitch inducible by arabinose and theophylline. false false _1526_ 0 18049 9 It's complicated false There cannot be a scar between the end of the riboswitch and the start codon of the TAL repressor. false Amy Soon component2373154 1 BBa_K1212004 component2373148 1 BBa_K206000 component2373151 1 BBa_K1212001 annotation2373154 1 BBa_K1212004 range2373154 1 205 2620 annotation2373151 1 BBa_K1212001 range2373151 1 139 198 annotation2373148 1 BBa_K206000 range2373148 1 1 130 BBa_K1212014_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggaggtaacaacaagtactagatggatccctccgacgcttcgccggccgcgcaggtggatctacgcacgctcggctacagtcagcagcagcaagagaagatcaaaccgaaggtgcgttcgacagtggcgcagcaccacgaggcactggtgggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacgtatcagcacataatcacggcgttgccagaggcgacacacgaagacatcgttggcgtcggcaaacagtggtccggcgcacgcgccctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaaacgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttacgccgcagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataaggggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgcaagcaatggcggtggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggcaatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcaacaggtggtagccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtagcgatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatggcggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataagggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacgggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtggccatcgccagcaatattggcggcaggccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgccatggatgcagtgaaaaagggactgccgcacgcgccggaattgatcagaagagtcaatcgccgtccggatcctaccggttgagctt BBa_K1212001_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggaggtaacaacaag BBa_K1212004_sequence 1 atggatccctccgacgcttcgccggccgcgcaggtggatctacgcacgctcggctacagtcagcagcagcaagagaagatcaaaccgaaggtgcgttcgacagtggcgcagcaccacgaggcactggtgggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacgtatcagcacataatcacggcgttgccagaggcgacacacgaagacatcgttggcgtcggcaaacagtggtccggcgcacgcgccctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaaacgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttacgccgcagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataaggggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgcaagcaatggcggtggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggcaatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcaacaggtggtagccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtagcgatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgcttccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatggcggtggcaagcaggcgctggagacggtgcagcgattgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagactgtccagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaataagggtggcaagcaggcgcttgagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggctctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgacgggggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccgcagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcaggcgctgttgccggtgctgtgccaggcccatggcctgacaccccagcaggtggtggccatcgccagcaatattggcggcaggccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgccatggatgcagtgaaaaagggactgccgcacgcgccggaattgatcagaagagtcaatcgccgtccggatcctaccggttgagctt BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z