LOCUS       BBa_K1212016            1003 bp    DNA     linear       27-Dec-2024
DEFINITION  pTet + TBS2 + RBS + GFP + T
ACCESSION   BBa_K1212016
VERSION     BBa_K1212016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..81
                     /label=BBa_K1212005
     misc_feature    94..97
                     /label=conserved
     RBS             90..101
                     /label=BBa_B0034
     misc_feature    824..866
                     /label=LVA tag
     misc_feature    755..755
                     /label=Bsa I fix
     misc_feature    108..866
                     /label=BBa_K1212008
     stem_loop       886..929
                     /label=stem_loop
     misc_feature    875..954
                     /label=BBa_B0010
     terminator      875..954
                     /label=BBa_B0010
     stem_loop       970..989
                     /label=T7 TE
     misc_feature    996..996
                     /label=stop
     polyA_site      990..1003
                     /label=polya
     misc_feature    963..1003
                     /label=BBa_B0012
     terminator      963..1003
                     /label=BBa_B0012
     terminator      875..1003
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtgagtgcg ggagcgtggg gtactagaga aagaggagaa atactagatg cgtaaaggag
      121 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      181 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      241 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      301 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      361 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      421 actacaagac acgtgctgaa gtcaagtttg aaggtggtac ccttgttaat agaatcgagt
      481 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      541 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      601 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      661 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      721 ctgccctttc gaaagatccc aacgaaaaga gagatcacat ggtccttctt gagtttgtaa
      781 cagctgctgg gattacacat ggcatggatg aactatacaa aaggcctgct gcaaacgacg
      841 aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct
      901 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      961 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//