##gff-version 3
##sequence-region BBa_K1212016 1 1003
BBa_K1212016	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1212016	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1212016	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1212016	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1212016	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1212016	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1212016	.	sequence_feature	63	81	.	+	0	ID=BBa_K1212005;Name=BBa_K1212005
BBa_K1212016	.	engineered_region	108	866	.	+	0	ID=BBa_K1212008;Name=BBa_K1212008
BBa_K1212016	.	tag	824	866	.	+	0	ID=annotation2347651;Name=LVA tag;Parent=BBa_K1212008
BBa_K1212016	.	sequence_alteration	755	755	.	+	0	ID=annotation2347666;Name=Bsa I fix;Parent=BBa_K1212008
BBa_K1212016	.	ribosome_entry_site	90	101	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1212016	.	conserved_region	94	97	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1212016	.	terminator	875	1003	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1212016	.	terminator	875	954	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1212016	.	engineered_region	875	954	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1212016	.	stem_loop	886	929	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1212016	.	terminator	963	1003	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1212016	.	engineered_region	963	1003	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1212016	.	stop_codon	996	996	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1212016	.	stem_loop	970	989	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1212016	.	polyA_site	990	1003	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1212016
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtgagtgcgggagcgtgg
ggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata