LOCUS BBa_K1212017 3631 bp DNA linear 15-Jun-2024 DEFINITION pTet+TBS2+RBS+GFP+T+pBAD+RS1+TAL1 ACCESSION BBa_K1212017 VERSION BBa_K1212017.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 63..81 /label=BBa_K1212005 misc_feature 94..97 /label=conserved RBS 90..101 /label=BBa_B0034 misc_feature 824..866 /label=LVA tag misc_feature 755..755 /label=Bsa I fix misc_feature 108..866 /label=BBa_K1212008 stem_loop 886..929 /label=stem_loop misc_feature 875..954 /label=BBa_B0010 terminator 875..954 /label=BBa_B0010 stem_loop 970..989 /label=T7 TE misc_feature 996..996 /label=stop polyA_site 990..1003 /label=polya misc_feature 963..1003 /label=BBa_B0012 terminator 963..1003 /label=BBa_B0012 terminator 875..1003 /label=BBa_B0015 misc_feature 1..1003 /label=BBa_K1212016 promoter 1012..1142 /label=promoter misc_feature 1051..1068 /label=AraI1 misc_feature 1072..1089 /label=AraI2 promoter 1012..1141 /label=BBa_K206000 stem_loop 1150..1187 /label=Theophylline aptamer RBS 1193..1200 /label=RBS misc_feature 1150..1209 /label=BBa_K1212001 misc_feature 3628..3631 /label=Golden Gate Assembly Scar CDS 1216..3627 /label=TAL repressor CDS 1216..3631 /label=BBa_K1212004 misc_feature 1012..3631 /label=BBa_K1212014 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtgagtgcg ggagcgtggg gtactagaga aagaggagaa atactagatg cgtaaaggag 121 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 181 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 241 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg 301 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca 361 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga 421 actacaagac acgtgctgaa gtcaagtttg aaggtggtac ccttgttaat agaatcgagt 481 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact 541 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact 601 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 661 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 721 ctgccctttc gaaagatccc aacgaaaaga gagatcacat ggtccttctt gagtttgtaa 781 cagctgctgg gattacacat ggcatggatg aactatacaa aaggcctgct gcaaacgacg 841 aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct 901 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 961 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacattgatt 1021 atttgcacgg cgtcacactt tgctatgcca tagcaagata gtccataaga ttagcggatc 1081 ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt tttgggctag 1141 ctactagagg gtgataccag catcgtcttg atgcccttgg cagcaccctg ctaaggaggt 1201 aacaacaagt actagatgga tccctccgac gcttcgccgg ccgcgcaggt ggatctacgc 1261 acgctcggct acagtcagca gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg 1321 gcgcagcacc acgaggcact ggtgggccat gggtttacac acgcgcacat cgttgcgctc 1381 agccaacacc cggcagcgtt agggaccgtc gctgtcacgt atcagcacat aatcacggcg 1441 ttgccagagg cgacacacga agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc 1501 gccctggagg ccttgctcac ggtggcggga gagttgagag gtccaccgtt acagttggac 1561 acaggccaac ttctcaagat tgcaaaacgt ggcggcgtga ccgcagtgga ggcagtgcat 1621 gcatggcgca atgcactgac gggtgccccc ctgaacctta cgccgcagca ggtggtggcc 1681 atcgccagca atattggtgg caagcaggcg ctggagacgg tgcagcggct gcttccggtg 1741 ctgtgccagg cccatggcct gaccccggag caggtggtgg ccatcgccag caatattggt 1801 ggcaagcagg cgctggagac ggtgcagcga ttgttgccgg tgctgtgcca ggcccatggc 1861 ctgaccccgg agcaggtggt ggccatcgcc agcaatattg gtggcaagca ggcgctggag 1921 actgtccagc ggctgttgcc ggtgctgtgc caggcccatg gcctgacccc ggagcaggtg 1981 gtggccatcg ccagccacga cggtggcaag caggcgcttg agacggtgca gcggctgttg 2041 ccggtgctgt gccaggccca tggcctgacc ccggagcagg tggtggccat cgccagcaat 2101 attggtggca agcaggctct ggagacggtg cagcggctgt tgccggtgct gtgccaggcc 2161 catggcctga ccccggagca ggtggtggcc atcgccagca ataagggggg caagcaggcg 2221 ctggagacgg tgcagcggct gttgccggtg ctgtgccagg cccatggcct gaccccgcag 2281 caggtggtgg ccatcgccag caatattggc ggcaagcagg cgctggagac ggtgcaggcg 2341 ctgttgccgg tgctgtgcca ggcccatggc ctgaccccgg agcaggtggt ggccatcgca 2401 agcaatggcg gtggcaagca ggcgctggag acggtgcagg cgctgttgcc ggtgctgtgc 2461 caggcccatg gcctgacccc ggagcaggtg gtggcaatcg ccagcaatat tggtggcaag 2521 caggcgctgg agacggtgca gcggctgttg ccggtgctgt gccaggccca tggcctgacc 2581 ccgcaacagg tggtagccat cgccagcaat attggtggca agcaggcgct ggagacggtg 2641 cagcggctgt tgccggtgct gtgccaggcc catggcctga caccccagca ggtggtagcg 2701 atcgccagca atattggtgg caagcaggcg ctggagacgg tgcagcggct gcttccggtg 2761 ctgtgccagg cccatggcct gaccccgcag caggtggtgg ccatcgccag caatggcggt 2821 ggcaagcagg cgctggagac ggtgcagcga ttgttgccgg tgctgtgcca ggcccatggc 2881 ctgaccccgg agcaggtggt ggccatcgcc agcaatattg gtggcaagca ggcgctggag 2941 actgtccagc ggctgttgcc ggtgctgtgc caggcccatg gcctgacccc ggagcaggtg 3001 gtggccatcg ccagcaataa gggtggcaag caggcgcttg agacggtgca gcggctgttg 3061 ccggtgctgt gccaggccca tggcctgacc ccggagcagg tggtggccat cgccagcaat 3121 attggtggca agcaggctct ggagacggtg cagcggctgt tgccggtgct gtgccaggcc 3181 catggcctga ccccggagca ggtggtggcc atcgccagcc acgacggggg caagcaggcg 3241 ctggagacgg tgcagcggct gttgccggtg ctgtgccagg cccatggcct gaccccgcag 3301 caggtggtgg ccatcgccag caatattggc ggcaagcagg cgctggagac ggtgcaggcg 3361 ctgttgccgg tgctgtgcca ggcccatggc ctgacacccc agcaggtggt ggccatcgcc 3421 agcaatattg gcggcaggcc ggcgctggag agcattgttg cccagttatc tcgccctgat 3481 ccggcgttgg ccgcgttgac caacgaccac ctcgtcgcct tggcctgcct cggcggacgt 3541 cctgccatgg atgcagtgaa aaagggactg ccgcacgcgc cggaattgat cagaagagtc 3601 aatcgccgtc cggatcctac cggttgagct t //