BBa_K1212022 1 BBa_K1212022 J23101+TBS2+GFP 2013-10-24T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution Strong promoter from the Anderson Family, J23101, with TALe binding site 2 controls the expression of GFP. false false _1526_ 0 18049 9 Not in stock false . false Amy Soon component2370656 1 BBa_J23101 component2370667 1 BBa_K1212020 annotation2370656 1 BBa_J23101 range2370656 1 1 35 annotation2370667 1 BBa_K1212020 range2370667 1 44 974 BBa_K1212019 1 BBa_K1212019 GFP+LVA tag + B0015 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution kit plate GFP+LVA tag + B0015 with BsaI site removed and Golden Gate Assembly site used in our construction. false false _1526_ 0 18049 9 It's complicated false . false Amy Soon component2348089 1 BBa_K1212018 component2348086 1 BBa_K1212008 annotation2348089 1 BBa_K1212018 range2348089 1 768 900 annotation2348086 1 BBa_K1212008 range2348086 1 1 759 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1212020 1 BBa_K1212020 TBS2+RBS+GFP+T 2013-10-24T11:00:00Z 2015-05-08T01:09:41Z iGEM distrubution TALe Binding Site 2, which corresponds to TAL 8 in front of a strong RBS, GFP with an LVA tag, and a double terminator. false false _1526_ 0 18049 9 Not in stock false . false Amy Soon component2370655 1 BBa_K1212019 component2370648 1 BBa_B0034 component2370646 1 BBa_K1212005 annotation2370648 1 BBa_B0034 range2370648 1 20 31 annotation2370646 1 BBa_K1212005 range2370646 1 1 19 annotation2370655 1 BBa_K1212019 range2370655 1 32 931 BBa_K1212018 1 BBa_K1212018 B0015 terminator 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z iGEM distribution kit plate B0015 terminator with overhang used in our Golden Gate Assembly. false false _1526_ 0 18049 9 It's complicated false . false Amy Soon annotation2348075 1 Golden Gate Assembly Site range2348075 1 130 133 annotation2348074 1 B0015 range2348074 1 1 129 BBa_K1212008 1 BBa_K1212008 Golden Gate compatible GFP 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z DNA from iGEM 2013 distribution K145015 with LVA tag fixed for Golden Gate compatibility through site directed mutagenesis. false false _1526_ 0 18052 9 Not in stock false - false Aura Ferreiro annotation2347666 1 Bsa I fix range2347666 1 648 648 annotation2347651 1 LVA tag range2347651 1 717 759 BBa_K1212005 1 BBa_K1212005 TALe Binding Site 2 (specific to TAL repressor 2) 2013-09-15T11:00:00Z 2015-05-08T01:09:41Z Synthetic. Assembled in the Segal Lab at UC Davis GBSF. See: 'Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects' by Meckler et al., submitted to Nucleic Acids Research for review. DNA binding sequence for transcriptional activator like (TAL) repressor 2 (part BBa_K1212006). High binding affinity. false false _1526_ 0 18052 9 Not in stock false - false Aura Ferreiro BBa_B0034_sequence 1 aaagaggagaaa BBa_K1212020_sequence 1 tgagtgcgggagcgtggggaaagaggagaaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212018_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212022_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagtgagtgcgggagcgtggggaaagaggagaaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212008_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa BBa_K1212019_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtggtacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt BBa_K1212005_sequence 1 tgagtgcgggagcgtgggg BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z